Detection of α-fetoprotein and glypican-3 mRNAs in the peripheral blood of hepatocellular carcinoma patients by using multiple FQ-RT-PCR

Journal of Clinical Laboratory Analysis

Volumn 25, Issue 2, 2011, Pages 113-117

  Yan, Dong ^a   He, Qingfang ^b   Chen, Yaping ^b   Wang, Lixin ^b   Zhang, Xinwei ^b  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG PROVINCIAL CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

Author keywords

AFP mRNA; Fluorescence quantitative reverse transcriptase polymerase chain reaction; GPC3 mRNA; Liver tumor; Peripheral blood

Indexed keywords

ALPHA FETOPROTEIN; BETA ACTIN; GLYPICAN 3; MESSENGER RNA;

ADULT; AGED; ARTICLE; BLOOD SAMPLING; CANCER PATIENT; CELL STRAIN HEPG2; CONTROLLED STUDY; EARLY DIAGNOSIS; FEMALE; FLUORESCENCE ANALYSIS; FLUORESCNCE QUANTITATIVE REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; HEPATITIS B; HUMAN; HUMAN CELL; LIVER CELL CARCINOMA; LIVER CIRRHOSIS; LIVER HEMANGIOMA; MAJOR CLINICAL STUDY; MALE; METASTASIS; OUTCOME ASSESSMENT; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS;

ADULT; AGED; AGED, 80 AND OVER; ALPHA-FETOPROTEINS; CARCINOMA, HEPATOCELLULAR; FEMALE; GENE EXPRESSION REGULATION, NEOPLASTIC; GLYPICANS; HEMANGIOMA; HEPATITIS B; HUMANS; LIVER CIRRHOSIS; LIVER NEOPLASMS; MALE; MIDDLE AGED; NEOPLASM PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TUMOR MARKERS, BIOLOGICAL; YOUNG ADULT;

EID: 79952648569     PISSN: 08878013     EISSN: 10982825     Source Type: Journal    
DOI: 10.1002/jcla.20443     Document Type: Article

References (9)

1. Chinese Anti-Cancer Association. Diagnostic criteria of primary liver cancer. Chin J Hepatol 2000;8:135.
2. Lemoine A, Le Bricon T, Salvucci M, et al. Prospective evaluation of circulating hepatocytes by alpha-fetoprotein mRNA in humans during liver surgery. Ann Surg 1997;226:43-50.
3. Nakatsura T, Yoshitake Y, Senju S, et al. Glypican-3, overexpressed specifically in human hepatocellular carcinoma, is a novel tumor marker. Biochem Biophys Res Commun 2003;306:16-25.
4. Sung YK, Hwang SY, Park MK, et al. Glypican-3 is overexpressed in human hepatocellular carcinoma. Cancer Sci 2003;94:259-262.
5. Matsumura M, Niwa Y, Kato N, et al. Detection of alpha-fetoprotein mRNA, an indicator of hematogenous spreading hepatocellular carcinoma, in the circulation: A possible predictor of metastatic hepatocellular carcinoma. Hepatology 1994;20:1418-1425.
6. Capurro M, Wanless IR, Sherman M, et al. Glypican-3: A novel serum and histochemical marker for hepatocellular carcinoma. Gastroenterology 2003;125:89-97.
7. Hippo Y, Watanabe K, Watanabe A, et al. Identification of soluble NH2-terminal fragment of glypican-3 as a serological marker for early-stage hepatocellular carcinoma. Cancer Res 2004;64:2418-2423.
8. Hsu HC, Cheng W, Lai PL. Cloning and expression of a developmentally regulated transcript MXR7 in hepatocellular carcinoma: Biological significance and temporospatial distribution. Cancer Res 1997;57:5179-5184.
9. Ding GH, Wang HY, Chen H, Wu MC. Expression of GPC3 gene in AFP-negative hepatoma and its clinicalimplication. Chin J Exp Surg 2001;18:112-113.