Isolation of RNP Granules

Methods in Molecular Biology

Volumn 703, Issue , 2011, Pages 265-273

  Jønson, Lars ^a   Nielsen, Finn Cilius ^a   Christiansen, Jan ^b  

^a Mental Health Services CPH   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

3X FLAG tag; post transcriptional operon; RNA binding protein; RNP granule; stable transfection; tetracycline induction

Indexed keywords

IMP 1 PROTEIN, HUMAN; IMP-1 PROTEIN, HUMAN; MESSENGER RNA; RIBONUCLEOPROTEIN; RNA BINDING PROTEIN; TETRACYCLINE;

ARTICLE; CELL GRANULE; GENE EXPRESSION REGULATION; GENETICS; IMMUNOPRECIPITATION; ISOLATION AND PURIFICATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING;

BLOTTING, WESTERN; CYTOPLASMIC GRANULES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION; IMMUNOPRECIPITATION; RIBONUCLEOPROTEINS; RNA, MESSENGER; RNA-BINDING PROTEINS; TETRACYCLINE;

MLCS; MLOWN;

EID: 79952588931     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-59745-248-9_18     Document Type: Chapter

References (8)

1. Keene, J. D. (2007) RNA regulons: coordination of post-transcriptional events. Nat Rev Genet 8, 533–543.
2. Gerber, A. P., Herschlag, D., Brown, P. O. (2004) Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol 2, E79.
3. Krichevsky, A. M., Kosik, K. S. (2001) Neuronal RNA granules: a link between RNA localization and stimulation-dependent translation. Neuron 32, 683–696.
4. Kanai, Y., Dohmae, N., Hirokawa, N. (2004) Kinesin transports RNA: isolation and characterization of an RNA-transporting granule. Neuron 43, 513–525.
5. Villace, P., Marion, R. M., Ortin, J. (2004) The composition of Staufen-containing RNA granules from human cells indicates their role in the regulated transport and translation of messenger RNAs. Nucleic Acids Res 32, 2411–2420.
6. Elvira, G., Wasiak, S., Blandford, V., Tong, X. K., Serrano, A., Fan, X., del Rayo Sanchez-Carbente, M., Servant, F., Bell, A. W., Bois-menu, D., Lacaille, J. C., McPherson, P. S., DesGroseillers, L., Sossin, W. S. (2006) Characterization of an RNA granule from developing brain. Mol Cell Proteomics 5, 635– 651.
7. Mili, S., Steitz, J. A. (2004) Evidence for reassociation of RNA-binding proteins after cell lysis: implications for the interpretation of immunoprecipitation analyses. RNA 10, 1692–1694.
8. Jonson, L., Vikesaa, J., Krogh, A., Nielsen, L. K., Hansen, T., Borup, R., Johnsen, A. H., Christiansen, J., Nielsen, F. C. (2007) Molecular composition of IMP1 ribonucleoprotein granules. Mol Cell Proteomics 6, 798–811.