Combined fluorescent in situ hybridization and immunofluorescence: Limiting factors and a substitution strategy for slide-mounted tissue sections

Journal of Neuroscience Methods

Volumn 196, Issue 2, 2011, Pages 281-288

  Nehmé, Benjamin ^a   Henry, Mélaine ^a   Mouginot, Didier ^a  

^a LAVAL UNIVERSITY   (Canada)

Author keywords

FISH; Immunofluorescence; Multiple fluorescent staining; Proteinase K; Riboprobe mRNA stabilization; SSC; Thin tissue section; Tissue permeabilization

Indexed keywords

MESSENGER RNA; NEURON SPECIFIC NUCLEAR PROTEIN; PROTEIN; PROTEINASE K; TRANSCRIPTION FACTOR FOSB; TYROSINE 3 MONOOXYGENASE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN NUCLEUS; BRAIN TISSUE; FLUORESCENCE IN SITU HYBRIDIZATION; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; ISOTOPE LABELING; NERVE CELL NETWORK; NEUROANATOMY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; RAT; RELIABILITY;

ANIMALS; FLUORESCENT ANTIBODY TECHNIQUE; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROTOMY; NEUROANATOMY; RATS; RATS, WISTAR; STAINING AND LABELING;

EID: 79952532524     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2011.01.018     Document Type: Article

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