PEGylation prevents bacteria-induced platelet activation and biofilm formation in platelet concentrates

Vox Sanguinis

Volumn 100, Issue 3, 2011, Pages 336-339

  Greco, C A ^a   Maurer Spurej, E ^a,b   Scott, M D ^a,b   Kalab, M ^c   Nakane, N ^a,b   Ramirez Arcos S M ^a  

^a CANADIAN BLOOD SERVICES   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c AGRICULTURE AND AGRI FOOD CANADA   (Canada)

Author keywords

Biofilms; Methoxypoly(ethylene glycol); PEGylation; Platelet concentrates; Staphylococcus epidermidis

Indexed keywords

MACROGOL DERIVATIVE; METHOXYPOLYETHYLENE GLYCOL; THROMBOCYTE CONCENTRATE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM; BACTERIUM CONTAMINATION; BIOFILM; HUMAN; INOCULATION; PRIORITY JOURNAL; STAPHYLOCOCCUS EPIDERMIDIS; THROMBOCYTE ACTIVATION; THROMBOCYTE AGGREGATION;

BACTERIA; BACTERIAL ADHESION; BIOFILMS; BLOOD PLATELETS; BLOOD PRESERVATION; HUMANS; PLATELET ACTIVATION; PLATELET TRANSFUSION; POLYETHYLENE GLYCOLS; STAPHYLOCOCCUS EPIDERMIDIS;

EID: 79952515203     PISSN: 00429007     EISSN: 14230410     Source Type: Journal    
DOI: 10.1111/j.1423-0410.2010.01419.x     Document Type: Article

References (12)

1. Scott MD, Murad KL, Koumpouras F, et al. Chemical camouflage of antigenic determinants: stealth erythrocytes. Proc Natl Acad Sci USA 1997; 94:7566-7571
2. Murad KL, Mahany KL, Brugnara C, et al. Structural and functional consequences of antigenic modulation of red blood cells with methoxypoly(ethylene glycol). Blood 1999; 93:2121-2127
3. Murad KL, Gosselin EJ, Eaton JW, et al. Stealth cells: prevention of major histocompatibility complex class II-mediated T-cell activation by cell surface modification. Blood 1999; 94:2135-2141
4. Chen AM, Scott MD: Immunocamouflage: prevention of transfusion-induced graft-versus-host disease via polymer grafting of donor cells. J Biomed Mater Res A 2003; 67:626-636
5. Rossi NA, Constantinescu I, Brooks DE, et al. Enhanced cell surface polymer grafting in concentrated and non-reactive aqueous polymer solutions. J Am Chem Soc 2010; 132:3423-3430
6. Walther-Wenke G: Incidence of bacterial transmission and transfusion reactions by blood components. Clin Chem Lab Med 2008; 46:919-925
7. Greco C, Martincic I, Gusinjac A, et al. Staphylococcus epidermidis forms biofilms under simulated platelet storage conditions. Transfusion 2007; 47:1143-1153
8. Greco CA, Zhang JG, Kalab M, et al. Effect of platelet additive solution on bacterial dynamics and their influence on platelet quality in stored platelet concentrates. Transfusion 2010; DOI:
9. Le Y, Scott MD: Immunocamouflage: the biophysical basis of immunoprotection by grafted methoxypoly(ethylene glycol) (mPEG). Acta Biomater 2010; 6:2631-2641
10. Brennan MP, Loughman A, Devocelle M, et al. Elucidating the role of Staphylococcus epidermidis serine-aspartate repeat protein G in platelet activation. J Thromb Haemost 2009; 7:1364-1372
11. Coller BS, Shattil SJ: The GPIIb/IIIa odyssey: a technology-driven saga of a receptor with twists, turns, and even a bend. Blood 2008; 112:3011-3025
12. Blair P, Rex S, Vitseva O, et al. Stimulation of toll-like receptor 2 in human platelets induces a thromboinflammatory response through activation of phosphoinositide 3-kinase. Circul Res 2009; 104:346-354