Identification and evaluation of soft coral diterpenes as inhibitors of HIF-2α induced gene expression

Bioorganic and Medicinal Chemistry Letters

Volumn 21, Issue 7, 2011, Pages 2113-2115

  Grkovic, Tanja ^a   Whitson, Emily L ^a   Rabe, Daniel C ^b   Gardella, Roberta S ^a,c   Bottaro, Donald P ^b   Linehan, W Marston ^b   McMahon, James B ^a   Gustafson, Kirk R ^a   McKee, Tawnya C ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

^c SAIC FREDERICK INC   (United States)

Author keywords

Alcyonaceae; Asterospicularia laurae; Bioassay guided fractionation; Cembrane; HIF; Lobophytum sarcophytoides; Marine natural products; Renal carcinoma; Sarcophyton sp.; Soft coral

Indexed keywords

1 (2 METHOXYPROPAN 2 YL) 4,8,12 TRIMETHYLOXABICYCLO[12.1.0]PENTADECA 1,3,11 TRIENE; 9 HYDROXY 1 (PROP 1 EN 2 YL) 8,12 DIMETHYL OXABICYCLO[9.3.2]HEXADECA 4,12,14 TRIEN 18 ONE; ACETIC ACID; ASTEROSPICULARIA LAURAE EXTRACT; DITERPENE; HYPOXIA INDUCIBLE FACTOR 2ALPHA; LOBOPHYTUM SARCOPHYTOIDES EXTRACT; METHANOL; NATURAL PRODUCT; SARCOPHYTON EXTRACT; UNCLASSIFIED DRUG;

ARTICLE; ASTEROSPICULARIA LAURAE; BIOASSAY; CORAL; DRUG DETERMINATION; DRUG ISOLATION; DRUG STRUCTURE; DRUG TARGETING; FRACTIONATION; GENE EXPRESSION REGULATION; HIGH THROUGHPUT SCREENING; IC 50; KIDNEY CARCINOMA; LOBOPHYTUM SARCOPHYTOIDES; NONHUMAN; SARCOPHYTON; SOLVENT EXTRACTION;

ANIMALS; ANTHOZOA; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS; DITERPENES; MAGNETIC RESONANCE SPECTROSCOPY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

ALCYONACEA; ASTEROSPICULARIA; LOBOPHYTUM; SARCOPHYTON;

EID: 79952483977     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2011.01.127     Document Type: Article

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24. 3 In brief, the assay used the renal cell carcinoma cell line 786-O engineered with five copies of the HIF-2 hypoxia responsive element (HRE) linked to a luciferase reporter gene. 5000 cells/well were plated in 384 well white plates and left to attach overnight. The following day, cells were treated with test samples or controls and the plates were incubated for a day to allow for HIF-2 signal to decrease. Following the incubation, luciferin was added to the cells and the luciferase signal measured. Treated cells were monitored in parallel for sample cytotoxicity using XTT colorimetric metabolic dye.