Oct-4B isoform is differentially expressed in breast cancer cells: Hypermethylation of regulatory elements of Oct-4A suggests an alternative promoter and transcriptional start site for Oct-4B transcription

Bioscience Reports

Volumn 31, Issue 2, 2011, Pages 109-115

  Wang, Yajuan ^a   Meng, Lu ^a   Hu, Hongyu ^a   Zhang, Ying ^a   Zhao, Chenfu ^a   Li, Qianqian ^a   Shi, Fangyu ^a   Wang, Xudong ^a   Lin, Aixing ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Alternative transcription; Cis regulatory element; DNA methylation; Epigenetics; MCF 7 cells; Oct 4

Indexed keywords

OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR OCT4B; UNCLASSIFIED DRUG; ISOPROTEIN; POU5F1 PROTEIN, HUMAN; TRANSCRIPTION FACTOR;

ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CONTROLLED STUDY; DNA METHYLATION; ENHANCER REGION; EPIGENETICS; NUCLEOTIDE SEQUENCE; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN METHYLATION; REGULATORY SEQUENCE; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION REGULATION; BREAST TUMOR; FEMALE; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE SILENCING; GENETIC EPIGENESIS; GENETICS; HUMAN; METABOLISM; POLYMERASE CHAIN REACTION; TUMOR CELL LINE;

BREAST NEOPLASMS; CELL LINE, TUMOR; DNA METHYLATION; EPIGENESIS, GENETIC; FEMALE; GENE EXPRESSION; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE SILENCING; HUMANS; OCTAMER TRANSCRIPTION FACTOR-3; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; PROTEIN ISOFORMS; REGULATORY ELEMENTS, TRANSCRIPTIONAL; REGULATORY SEQUENCES, NUCLEIC ACID; TRANSCRIPTION FACTORS; TRANSCRIPTION INITIATION SITE;

EID: 79952465629     PISSN: 01448463     EISSN: None     Source Type: Journal    
DOI: 10.1042/BSR20100033     Document Type: Article

References (31)

1. Babaie, Y., Herwig, R., Greber, B., Brink, T. C., Wruck, W., Groth, D., Lehrach, H., Burdon, T. and Adjaye, J. (2007) Analysis of Oct4-dependent transcriptional networks regulating self-renewal and pluripotency in human embryonic stem cells. Stem Cells 25, 500-510 (Pubitemid 46232806)
2. Pan, G. J., Chang, Z. Y., Scholer, H. R. and Pei, D. (2002) Stem cell pluripotency and transcription factor Oct4. Cell Res. 12, 321-329 (Pubitemid 135708344)
3. Takeda, J., Seino, S. and Bell, G. I. (1992) Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues. Nucleic Acids Res. 20, 4613-4620
4. Atlasi, Y., Mowla, S. J., Ziaee, S., Gokhale, P. J. and Andrews, P. W. (2008) OCT4 spliced variants are differentially expressed in human pluripotent and nonpluripotent cells. Stem Cells 26, 3068-3074
5. Nordhoff, V., Hubner, K., Bauer, A., Orlova, I., Malapetsa, A. and Scholer, H. R. (2001) Comparative analysis of human, bovine, and murine Oct-4 upstream promoter sequences. Mamm. Genome 12, 309-317
6. Gu, P., Goodwin, B., Chung, A. C., Xu, X., Wheeler, D. A., Price, R. R., Galardi, C., Peng, L., Latour, A. M., Koller, B. H. et al. (2005) Orphan nuclear receptor LRH-1 is required to maintain Oct4 expression at the epiblast stage of embryonic development. Mol. Cell. Biol. 25, 3492-3505
7. Gu, P., LeMenuet, D., Chung, A. C., Mancini, M., Wheeler, D. A. and Cooney, A. J. (2005) Orphan nuclear receptor GCNF is required for the repression of pluripotency genes during retinoic acid-induced embryonic stem cell differentiation. Mol. Cell. Biol. 25, 8507-8519
8. Liu, N., Lu, M., Tian, X. and Han, Z. (2007) Molecular mechanisms involved in self-renewal and pluripotency of embryonic stem cells. J. Cell. Physiol. 211, 279-286
9. Zhang, J., Tam, W. L., Tong, G. Q., Wu, Q., Chan, H. Y., Soh, B. S., Lou, Y., Yang, J., Ma, Y., Chai, L. et al. (2006) Sall4 modulates embryonic stem cell pluripotency and early embryonic development by the transcriptional regulation of Pou5fl. Nat. Cell Biol. 8, 1114-1123
10. Hattori, N., Nishino, K., Ko, Y., Hattori, N., Ohgane, J., Tanaka, S. and Shiota, K. (2004) Epigenetic control of mouse Oct-4 gene expression in ES cells and TS cells. J. Biol. Chem. 279, 17063-17069
11. Latham, T., Gilbert, N. and Ramsahoye, B. (2008) DNA methylation in mouse embryonic stem cells and development. Cell Tissue Res. 331, 31-55 (Pubitemid 50006457)
12. Freberg, C. T., Dahl, J. A., Timoskainen, S. and Collas, P. (2007) Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract. Mol. Biol. Cell 18, 1543-1553
13. Zhang, H. J., Siu, M., Wong, E., Wong, K. Y., Li, A., Chan, K., Ngan, H. and Cheung, A. (2008) Oct4 is epigenetically regulated by methylation in normal placenta and gestational trophoblastic disease. Placenta 29, 549-554
14. Cantz, T., Key, G., Bleidissel, M., Gentile, L., Han, D. W., Brenne, A. and Scholer, H. R. (2008) Absence of OCT4 expression in somatic tumor cell lines. Stem Cells 26, 692-697
15. Cauffman, G., Liebaers, I., van Steirteghem, A. and van de Velde, H. (2006) POU5F1 isoforms show different expression patterns in human embryonic stem cells and preimplantation embryos. Stem Cells 24, 2685-2691 (Pubitemid 46053006)
16. Papamichos, S. I., Kotoula, V., Tarlatzis, B. C., Agorastos, T., Papazisis, K. and Lambropoulos, A. F. (2009) OCT4B1 isoform: the novel OCT4 alternative spliced variant as a putative marker of sternness. Mol. Hum. Reprod. 15, 269-270
17. Liedtke, S., Stephan, M. and Kogler, G. (2008) Oct4 expression revisited: potential pitfalls for data misinterpretation in stem cell research. Biol. Chem. 389, 845-850
18. Suo, G., Han, J., Wang, X., Zhang, J., Zhao, Y., Zhao, Y. and Dai, J. (2005) Oct4 pseudogenes are transcribed in cancers. Biochem. Biophys. Res. Commun. 337, 1047-1051
19. Liedtke, S., Enczmann, J., Waclawczyk, S., Wernet, P. and Kogler, G. (2007) Oct4 and its pseudogenes confuse stem cell research. Cell Stem Cell 1, 364-366 (Pubitemid 47506539)
20. Kaltz, N., Funari, A., Hippauf, S., Delorme, B., Noel, D., Riminucci, M., Jacobs, V. R., Haupl, T., Jorgensen, C., Charbord, P. et al. (2008) In vivo osteoprogenitor potency of human stromal cells from different tissues does not correlate with expression of POU5F1 or its pseudogenes. Stem Cells 26, 2419-2424
21. Zhang, Y., Shi, F. Y., Gao, S. R. and Lin, A. X. (2009) Spatio-temporal expression of heterologous Oct-4 promoter in preimplantation embryos of pig, rabbit and mouse. Prog. Biochem. Biophys. 36, 1180-1185
22. Visvader, J. E. and Lindeman, G. J. (2008) Cancer stem cells in solid tumours: accumulating evidence and unresolved questions. Nat. Rev. Cancer 8, 755-768
23. Ezeh, U. I., Turek, P. J., Reijo, R. A. and Clark, A. T. (2005) Human embryonic stem cell genes OCT4, NANOG, STELLAR, and GDF3 are expressed in both seminoma and breast carcinoma. Cancer 04, 2255-2265
24. Hu, T., Liu, S., Breiter, D. R., Wang, F., Tang, Y. and Sun, S. (2008) Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis. Cancer Res. 68, 6533-6540
25. Wang, P., Branch, D. R., Bali, M., Schultz, G. A., Goss, P. E. and Jin, T. (2003) The POU homeodomain protein OCT3 as a potential transcriptional activator for fibroblast growth factor-4 (FGF-4) in human breast cancer cells. Biochem. J. 375, 199-205 (Pubitemid 37255396)
26. Zangrossi, S., Marabese, M., Broggini, M., Giordano, R., D'Erasmo, M., Montelatici, E., Intini, D., Neri, A., Pesce, M. and Rebulla, P. (2007) Oct-4 expression in adult human differentiated cells challenges its role as a pure stem cell marker. Stem Cells 25, 1675-1680 (Pubitemid 47121844)
27. Wang, X., Zhao, Y., Xiao, Z., Chen, B., Wei, Z., Wang, B., Zhang, J., Han, J., Gao, Y. and Li, L. (2009) Alternative translation of OCT4 by an internal ribosome entry site and its novel function in stress response. Stem Cells 27, 1265-1275
28. Tai, M. H., Chang, C. C., Olson, L. K. and Trosko, J. E. (2005) Oct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesis. Carcinogenesis 26, 495-502
29. Kotoula, V., Papamichos, S. I. and Lambropoulos, A. F. (2008) Revisiting OCT4 expression in peripheral blood mononuclear cells. Stem Cells 26, 290-291
30. Mallanna, S. K., Boer, B., Desler, M. and Rizzino, A. (2008) Differential regulation of the Oct-3/4 gene in cell culture model systems that parallel different stages of mammalian development. Mol. Reprod. Dev. 75, 1247-1257
31. Deb-Rinker, P., Ly, D., Jezierski, A., Sikorska, M. and Walker, P. R. (2005) Sequential DNA methylation of the Nanog and Oct-4 upstream regions in human NT 2 cells during neuronal differentiation. J. Biol. Chem. 280, 6257-6260