A new method to efficiently produce transgenic embryos and mice from low-titer lentiviral vectors

Transgenic Research

Volumn 20, Issue 2, 2011, Pages 357-363

  Miao, Kai ^a   Guo, Min ^a   An, Lei ^a   Xu, Xiao Ling ^a   Wu, Han ^b   Wang, Dong ^c   Wu, Zhong Hong ^a   Tian, Jian Hui ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF ZOOLOGY   (China)

^c INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Lentiviral vectors; Low titer; Mouse; Transgenic embryos

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ANIMAL; ANIMAL EMBRYO; ARTICLE; BIOTECHNOLOGY; BLASTOCYST; EVALUATION; FEMALE; GENE TRANSFER; GENE VECTOR; GENETIC TRANSDUCTION; GENETICS; LENTIVIRINAE; MALE; METABOLISM; METHODOLOGY; MICROMANIPULATION; MOUSE; PHYSIOLOGY; TRANSGENE; TRANSGENIC ANIMAL; TRANSGENIC MOUSE; VIROLOGY;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BIOTECHNOLOGY; BLASTOCYST; EMBRYO, MAMMALIAN; FEMALE; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LENTIVIRUS; MALE; MICE; MICE, TRANSGENIC; MICROMANIPULATION; TRANSDUCTION, GENETIC; TRANSGENES;

LENTIVIRUS; MAMMALIA; MUS;

EID: 79952455346     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-010-9414-5     Document Type: Article

References (19)

1. Anzai M, Nishiwaki M et al (2006) Application of laser-assisted zona drilling to in vitro fertilization of cryopreserved mouse oocytes with spermatozoa from a subfertile transgenic mouse. J Reprod Dev 52(5): 601-606.
2. Bushman F, Lewinski M et al (2005) Genome-wide analysis of retroviral DNA integration. Nat Rev Microbiol 3(11): 848-858.
3. Chan AW, Chong KY et al (2001) Transgenic monkeys produced by retroviral gene transfer into mature oocytes. Science 291(5502): 309-312.
4. Ewerling S, Hofmann A et al (2006) Evaluation of laser-assisted lentiviral transgenesis in bovine. Transgenic Res 15(4): 447-454.
5. Hamatani T, Carter MG et al (2004) Dynamics of global gene expression changes during mouse preimplantation development. Dev Cell 6(1): 117-131.
6. Hofmann A, Kessler B et al (2003) Efficient transgenesis in farm animals by lentiviral vectors. EMBO Rep 4(11): 1054-1060.
7. Hofmann A, Zakhartchenko V et al (2004) Generation of transgenic cattle by lentiviral gene transfer into oocytes. Biol Reprod 71(2): 405-409.
8. Kimura Y, Yanagimachi R (1995) Intracytoplasmic sperm injection in the mouse. Biol Reprod 52(4): 709-720.
9. Lois C, Hong EJ et al (2002) Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science 295(5556): 868-872.
10. McGrew MJ, Sherman A et al (2004) Efficient production of germline transgenic chickens using lentiviral vectors. EMBO Rep 5(7): 728-733.
11. Nakagata N, Okamoto M et al (1997) Positive effect of partial zona-pellucida dissection on the in vitro fertilizing capacity of cryopreserved C57BL/6 J transgenic mouse spermatozoa of low motility. Biol Reprod 57(5): 1050-1055.
12. Pfeifer A (2004) Lentiviral transgenesis. Transgenic Res 13(6): 513-522.
13. Pfeifer A, Hofmann A (2009) Lentiviral transgenesis. Methods Mol Biol 530: 391-405.
14. Pfeifer A, Ikawa M et al (2002) Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc Natl Acad Sci USA 99(4): 2140-2145.
15. Ritchie WA, Taylor JE et al (2005) Live lambs born from zona-pellucida denuded embryos. Cloning Stem Cells 7(3): 178-182.
16. Ritchie WA, Neil C et al (2007) Transgenic embryos and mice produced from low titre lentiviral vectors. Transgenic Res 16(5): 661-664.
17. Sauvain MO, Dorr AP et al (2008) Genotypic features of lentivirus transgenic mice. J Virol 82(14): 7111-7119.
18. Tiscornia G, Singer O et al (2006) Production and purification of lentiviral vectors. Nat Protoc 1(1): 241-245.
19. Whitelaw CB, Radcliffe PA et al (2004) Efficient generation of transgenic pigs using equine infectious anaemia virus (EIAV) derived vector. FEBS Lett 571(1-3): 233-236.