Precise beam-tilt alignment and collimation are required to minimize the phase error associated with coma in high-resolution cryo-EM

Journal of Structural Biology

Volumn 174, Issue 1, 2011, Pages 1-10

  Glaeser, Robert M ^a   Typke, Dieter ^a   Tiemeijer, Peter C ^b   Pulokas, James ^c   Cheng, Anchi ^c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Thermo Fisher Scientific   (United States)

^c SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Beam alignment; Coma; Phase error

Indexed keywords

ANALYTICAL ERROR; ELECTRON DIFFRACTION; ELECTRON MICROSCOPY; ILLUMINATION; OPTICAL RESOLUTION; PRIORITY JOURNAL; REVIEW;

CRYOELECTRON MICROSCOPY;

EID: 79952438103     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2010.12.005     Document Type: Review

References (27)

1. Chen J.Z., Settembre E.C., Aoki S.T., Zhang X., Bellamy A.R., Dormitzer P.R., Harrison S.C., Grigorieff N. Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM. Proceedings of the National Academy of Sciences of the United States of America 2009, 106:10644-10648.
2. Christenson K.K., Eades J.A. On parallel illumination in the transmission electron microscope. Ultramicroscopy 1986, 19:191-194.
3. Christenson K.K., Eades J.A. Skew thoughts on parallelism. Ultramicroscopy 1988, 26:113-132.
4. Cong Y., Baker M.L., Jakana J., Woolford D., Miller E.J., Reissmann S., Kumar R.N., Redding-Johanson A.M., Batth T.S., Mukhopadhyay A., Ludtke S.J., Frydman J., Chiu W. 4.0Å resolution cryo-EM structure of the mammalian chaperonin TRiC/CCT reveals its unique subunit arrangement. Proceedings of the National Academy of Sciences of the United States of America 2010, 107:4967-4972.
5. Eades A. Obtaining TEM images with a uniform deviation parameter. Ultramicroscopy 2006, 106:432-438.
6. Fernandez J.J., Luque D., Caston J.R., Carrascosa J.L. Sharpening high resolution information in single particle electron cryomicroscopy. Journal of Structural Biology 2008, 164:170-175.
7. Frank J. Envelope of electron-microscopic transfer-functions for partially coherent illumination. Optik 1973, 38:519-536.
8. Frank J. Three-Dimensional Electron Microscopy of Macromolecular Assemblies - Visualization of Biological Molecules in Their Native State 2006, Oxford University Press, New York. second ed.
9. Glaeser R.M., Downing K., DeRosier D., Chiu W., Frank J. Electron Crystallography of Biological Macromolecules 2007, Oxford University Press.
10. Henderson R., Glaeser R.M. Quantitative-analysis of image-contrast in electron-micrographs of beam-sensitive crystals. Ultramicroscopy 1985, 16:139-150.
11. Henderson R., Baldwin J.M., Downing K.H., Lepault J., Zemlin F. Structure of purple membrane from halobacterium-halobium - recording, measurement and evaluation of electron-micrographs at 3.5Å resolution. Ultramicroscopy 1986, 19:147-178.
12. Jiang W., Baker M.L., Jakana J., Weigele P.R., King J., Chiu W. Backbone structure of the infectious epsilon 15 virus capsid revealed by electron cryomicroscopy. Nature 2008, 451:1130-1134.
13. Koster A.J., de Ruijter W.J. Practical autoalignment of transmission electron microscopes. Ultramicroscopy 1992, 40:89-107.
14. Ludtke S.J., Baker M.L., Chen D.H., Song J.L., Chuang D.T., Chiu W. De novo backbone trace of GroEL from single particle electron cryomicroscopy. Structure 2008, 16:441-448.
15. Potter C.S., Chu H., Frey B., Green C., Kisseberth N., Madden T.J., Miller K.L., Nahrstedt K., Pulokas J., Reilein A., Tcheng D., Weber D., Carragher B. Leginon: a system for fully automated acquisition of 1000 electron micrographs a day. Ultramicroscopy 1999, 77:153-161.
16. Rose H.H. Geometrical Charged-Particle Optics 2009, Springer, Berlin.
17. Smith D.J., Saxton W.O., Okeefe M.A., Wood G.J., Stobbs W.M. The importance of beam alignment and crystal tilt in high-resolution electron-microscopy. Ultramicroscopy 1983, 11:263-281.
18. Suloway C., Pulokas J., Fellmann D., Cheng A., Guerra F., Quispe J., Stagg S., Potter C.S., Carragher B. Automated molecular microscopy: the new Leginon system. Journal of Structural Biology 2005, 151:41-60.
19. Typke D., Dierksen K. Determination of image aberrations in high-resolution electron microscopy using diffractogram and cross-correlation methods. Optik 1995, 99:155-166.
20. Typke D., Gilpin C.J., Downing K.H., Glaeser R.M. Stroboscopic image capture: reducing the dose per frame by a factor of 30 does not prevent beam-induced specimen movement in paraffin. Ultramicroscopy 2007, 107:106-115.
21. Wade R.H., Frank J. Electron-microscope transfer-functions for partially coherent axial illumination and chromatic defocus spread. Optik 1977, 49:81-92.
22. Wolf M., Garcea R.L., Grigorieff N., Harrison S.C. Subunit interactions in bovine papillomavirus. Proceedings of the National Academy of Sciences of the United States of America 2010, 107:6298-6303.
23. Yu X.K., Jin L., Zhou Z.H. 3.88Å Structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy. Nature 2008, 453:415-419.
24. Zemlin F., Weiss K., Schiske P., Kunath W., Herrmann K.H. Coma-free alignment of high-resolution electron-microscopes with aid of optical diffractograms. Ultramicroscopy 1978, 3:49-60.
25. Zhang X., Jin L., Fang Q., Hui W.H., Zhou Z.H. 3.3 Angstrom cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry. Cell 2010, 141:472-482.
26. Zhang X., Settembre E., Xu C., Dormitzer P.R., Bellamy R., Harrison S.C., Grigorieff N. Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction. Proceedings of the National Academy of Sciences of the United States of America 2008, 105:1867-1872.
27. Zhou Z.H. Towards atomic resolution structural determination by single-particle cryo-electron microscopy. Current Opinion in Structural Biology 2008, 18:218-228.