Elongation factor 1-alpha is released into the culture medium during growth of Giardia intestinalis trophozoites

Experimental Parasitology

Volumn 127, Issue 4, 2011, Pages 804-810

  Skarin, Hanna ^a   Ringqvist, Emma ^a   Hellman, Ulf ^b   Svärd, Staffan G ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b LUDWIG INSTITUTE FOR CANCER RESEARCH   (Sweden)

Author keywords

Elongation factor 1 alpha; Giardia; Intestinal parasite; Secretory excretory products; Virulence factor

Indexed keywords

ARGININE DEIMINASE; BOVINE SERUM ALBUMIN; ELONGATION FACTOR 1ALPHA; RECOMBINANT PROTEIN;

ARTICLE; CELL ADHESION; CELL NUCLEUS; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CULTURE MEDIUM; CYTOPLASM; GENOME ANALYSIS; GIARDIA LAMBLIA; HOST PARASITE INTERACTION; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULAR WEIGHT; NONHUMAN; PARASITE VIRULENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN SECRETION; SEQUENCE HOMOLOGY; TROPHOZOITE;

ANIMALS; BLOTTING, WESTERN; CELL LINE, TUMOR; CULTURE MEDIA, SERUM-FREE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FIBROBLASTS; GENE EXPRESSION; GIARDIA LAMBLIA; GIARDIASIS; HUMANS; HYDROLASES; IMMUNE SERA; MICROSCOPY, FLUORESCENCE; PEPTIDE ELONGATION FACTOR 1; PROMOTER REGIONS, GENETIC; RABBITS; RECOMBINANT PROTEINS; TRANSFECTION; TROPHOZOITES;

BOVINAE; GIARDIA; GIARDIA INTESTINALIS;

EID: 79952362325     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2011.01.006     Document Type: Article

References (45)

1. Ali S.A., Hill D.R. Giardia intestinalis. Current Opinion in Infectious Diseases 2003, 16:453-460.
2. Andrade M.A., Siles-Lucas M., Lopez-Aban J., Carranza C., Perez-Arellano J.L., Muro A. Antigens from Ascaris suum trigger in vitro macrophage NO production. Parasite Immunology 2005, 27:235-242.
3. Ankarklev J., Jerlström-Hultqvist J., Ringqvist E., Troell K., Svärd S.G. Behind the smile: cell biology and disease mechanisms of Giardia species. Nature Reviews Microbiology 2010, 8:413-422.
4. Buret A.G. Mechanisms of epithelial dysfunction in giardiasis. Gut 2007, 56:316-317.
5. Carrero J.C., Petrossian P., Acosta E., Sanchez-Zerpa M., Ortiz-Ortiz L., Laclette J.P. Cloning and characterization of Entamoeba histolytica antigens recognized by human secretory IgA antibodies. Parasitology Research 2000, 86:330-334.
6. Condeelis J. Elongation factor 1 alpha, translation and the cytoskeleton. Trends in Biochemical Sciences 1995, 20:169-170.
7. Davids B.J., Palm J.E.D., Housley M.P., Smith J.R., Andersen Y.S., Martin M.G., Hendrickson B.A., Johansen F.-E., Svärd S.G., Gillin F.D., Eckmann L. Polymeric immunoglobulin receptor in intestinal immune defense against the lumen-dwelling protozoan parasite Giardia. Journal of Immunology 2006, 177:6281-6290.
8. de Carvalho T.B., David E.B., Coradi S.T., Guimaraes S. Protease activity in extracellular products secreted in vitro by trophozoites of Giardia duodenalis. Parasitology Research 2008, 104:185-190.
9. Degnan B.A., Palmer J.M., Robson T., Jones C.E., Fischer M., Glanville M., Mellor G.D., Diamond A.G., Kehoe M.A., Goodacre J.A. Inhibition of human peripheral blood mononuclear cell proliferation by Streptococcus pyogenes cell extract is associated with arginine deiminase activity. Infection and Immunity 1998, 66:3050-3058.
10. Eckmann L., Laurent F., Langford T.D., Hetsko M.L., Smith J.R., Kagnoff M.F., Gillin F.D. Nitric oxide production by human intestinal epithelial cells and competition for arginine as potential determinants of host defense against the lumen-dwelling pathogen Giardia lamblia. Journal of Immunology 2000, 164:1478-1487.
11. Farthing M.J. The molecular pathogenesis of giardiasis. Journal of Pediatric Gastroenterology and Nutrition 1997, 24:79-88.
12. Franzen O., Jerlström-Hultqvist J., Castro E., Sherwood E., Ankarklev J., Reiner D.S., Palm D., Andersson J.O., Andersson B., Svärd S.G. Draft genome sequencing of Giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?. PLoS Pathogens 2009, 5:e1000560.
13. Ge J., Catt D.M., Gregory R.L. Streptococcus mutans surface alpha-enolase binds salivary mucin MG2 and human plasminogen. Infection and Immunity 2004, 72:6748-6752.
14. Granato D., Bergonzelli G.E., Pridmore R.D., Marvin L., Rouvet M., Corthesy-Theulaz I.E. Cell surface-associated elongation factor Tu mediates the attachment of Lactobacillus johnsonii NCC533 (La1) to human intestinal cells and mucins. Infection and Immunity 2004, 72:2160-2169.
15. Jerlström-Hultqvist J., Franzén O., Ankarklev J., Xu F., Nohýnková E., Andersson J.O., Svärd S.G., Andersson B. Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate. BMC Genomics 2010, 11:543.
16. Jimenez J.C., Fontaine J., Grzych J.M., Dei-Cas E., Capron M. Systemic and mucosal responses to oral administration of excretory and secretory antigens from Giardia intestinalis. Clinical and Diagnostic Laboratory Immunology 2004, 11:152-160.
17. Jimenez J.C., Morelle W., Michalsky J.C., Dei-Cas E. Excreted/secreted glycoproteins of G. intestinalis play an essential role in the antibody response. Parasitology Research 2007, 100:715-720.
18. Jimenez J.C., Uzcanga G., Zambrano A., Di Prisco M.C., Lynch N.R. Identification and partial characterization of excretory/secretory products with proteolytic activity in Giardia intestinalis. Journal of Parasitology 2000, 86:859-862.
19. Kaur H., Ghosh S., Samra H., Vinayak V.K., Ganguly N.K. Identification and characterization of an excretory-secretory product from Giardia lamblia. Parasitology 2001, 123:347-356.
20. Kaur H., Samra H., Ghosh S., Vinayak V.K., Ganguly N.K. Immune effector responses to an excretory-secretory product of Giardia lamblia. FEMS Immunology and Medical Microbiology 1999, 23:93-105.
21. Knodler L.A., Svard S.G., Silberman J.D., Davids B.J., Gillin F.D. Developmental gene regulation in Giardia lamblia: first evidence for an encystation-specific promoter and differential 5′ mRNA processing. Molecular Microbiology 1999, 34:327-340.
22. 2+/calmodulin. Journal of Biochemistry 1996, 119:791-798.
23. Ljungstrom I., Castor B. Immune response to Giardia lamblia in a water-borne outbreak of giardiasis in Sweden. Journal of Medical Microbiology 1992, 36:347-352.
24. Morrison H.G., McArthur A.G., Gillin F.D., Aley S.B., Adam R.D., Olsen G.J., Best A.A., Cande W.Z., Chen F., Cipriano M.J., Davids B.J., Dawson S.C., Elmendorf H.G., Hehl A.B., Holder M.E., Huse S.M., Kim U.U., Lasek-Nesselquist E., Manning G., Nigam A., Nixon J.E., Palm D., Passamaneck N.E., Prabhu A., Reich C.I., Reiner D.S., Samuelson J., Svärd S.G., Sogin M.L. Genomic minimalism in the early diverging intestinal parasite Giardia lamblia. Science 2007, 317:1921-1926.
25. Mundodi V., Kucknoor A.S., Alderete J.F. Immunogenic and plasminogen-binding surface-associated alpha-enolase of Trichomonas vaginalis. Infection and Immunity 2008, 76:523-531.
26. Murray J.W., Edmonds B.T., Liu G., Condeelis J. Bundling of actin filaments by elongation factor 1 alpha inhibits polymerization at filament ends. Journal of Cell Biology 1996, 135:1309-1321.
27. Nandan D., Cherkasov A., Sabouti R., Yi T., Reiner N.E. Molecular cloning, biochemical and structural analysis of elongation factor-1 alpha from Leishmania donovani: comparison with the mammalian homologue. Biochemical and Biophysical Research Communication 2003, 302:646-652.
28. Nandan D., Reiner N.E. Leishmania donovani engages in regulatory interference by targeting macrophage protein tyrosine phosphatase SHP-1. Clinical Immunology 2005, 114:266-277.
29. Nandan D., Lopez M., Ban F., Huang M., Li Y., Reiner N.E., Cherkasov A. Indel-based targeting of essential proteins in human pathogens that have close host orthologoue(s): discovery of selective inhibitors for Leishmania donovani elongation factor-1α. Proteins 2007, 67:53-64.
30. Palm D., Weiland M., McArthur A.G., Winiecka-Krusnell J., Cipriano M.J., Birkeland S.R., Pacocha S.E., Davids B., Gillin F., Linder E., Svärd S. Developmental changes in the adhesive disk during Giardia differentiation. Molecular and Biochemical Parasitology 2005, 141:199-207.
31. Palm J.E., Weiland M.E., Griffiths W.J., Ljungström I., Svärd S.G. Identification of immunoreactive proteins during acute human giardiasis. Journal of Infectious Diseases 2003, 187:1849-1859.
32. Pittman Y.R., Kandl K., Lewis M., Valente L., Kinzy T.G. Coordination of eukaryotic translation elongation factor 1A (eEF1A) function in actin organization and translation elongation by the guanine nucleotide exchange factor eEF1Balpha. Journal of Biological Chemistry 2009, 284:4739-4747.
33. Ringqvist E., Palm J.E., Skarin H., Hehl A.B., Weiland M., Davids B.J., Reiner D.S., Griffiths W.J., Eckmann L., Gillin F.D., Svärd S.G. Release of metabolic enzymes by Giardia in response to interaction with intestinal epithelial cells. Molecular and Biochemical Parasitology 2008, 159:85-91.
34. Rodriguez-Fuentes G.B., Cedillo-Rivera R., Fonseca-Linan R., Arguello-Garcia R., Munoz O., Ortega-Pierres G., Yepez-Mulia L. Giardia duodenalis: analysis of secreted proteases upon trophozoite-epithelial cell interaction in vitro. Memorias do Instituto Oswaldo Cruz 2006, 101:693-696.
35. Roxström-Lindquist K., Palm D., Reiner D., Ringqvist E., Svärd S.G. Giardia immunity - an update. Trends in Parasitology 2006, 22:26-31.
36. Roxström-Lindquist K., Ringqvist E., Palm D., Svärd S. Giardia lamblia-induced changes in gene expression in differentiated Caco-2 human intestinal epithelial cells. Infection and Immunity 2005, 73:8204-8208.
37. Salamitou S., Raynaud O., Lemaire M., Coughlan M., Beguin P., Aubert J.P. Recognition specificity of the duplicated segments present in Clostridium thermocellum endoglucanase CelD and in the cellulosome-integrating protein CipA. Journal of Bacteriology 1994, 176:2822-2827.
38. Samra H.K., Ganguly N.K., Garg U.C., Goyal J., Mahajan R.C. Effect of excretory-secretory products of Giardia lamblia on glucose and phenylalanine transport in the small intestine of Swiss albino mice. Biochemistry International 1988, 17:801-812.
39. Shant J., Bhattacharyya S., Ghosh S., Ganguly N.K., Majumdar S. A potentially important excretory-secretory product of Giardia lamblia. Experimental Parasitology 2002, 102:178-186.
40. Shant J., Ghosh S., Bhattacharyya S., Ganguly N.K., Majumdar S. The alteration in signal transduction parameters induced by the excretory-secretory product from Giardia lamblia. Parasitology 2004, 129:421-430.
41. Shevchenko A., Wilm M., Vorm O., Mann M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Analytical Chemistry 1996, 68:850-858.
42. Silverman J.M., Reiner N.E. Exosomes and other microvesicles in infection biology: organelles with unanticipated phenotypes. Cellular Microbiology 2010, 13:1-9.
43. Tan S., Tompkins L.S., Amieva M.R. Helicobacter pylori usurps cell polarity to turn the cell surface into a replicative niche. PLoS Pathogens 2009, 5:e1000407.
44. Teoh D.A., Kamieniecki D., Pang G., Buret A.G. Giardia lamblia rearranges F-actin and alpha-actinin in human colonic and duodenal monolayers and reduces transepithelial electrical resistance. Journal of Parasitology 2000, 86:800-806.
45. Troeger H., Epple H.J., Schneider T., Wahnschaffe U., Ullrich R., Burchard G.D., Jelinek T., Zeitz M., Fromm M., Schulzke J.D. Effect of chronic Giardia lamblia infection on epithelial transport and barrier function in human duodenum. Gut 2007, 56:328-335.