Homogenisation of cystic fibrosis sputum by sonication - An essential step for Aspergillus PCR

Journal of Microbiological Methods

Volumn 85, Issue 1, 2011, Pages 75-81

  Baxter, Caroline G ^a,b   Jones, Andrew M ^a,b   Webb, Kevin ^a,b   Denning, David W ^a,b  

^a MANCHESTER UNIVERSITY NHS FOUNDATION TRUST   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Aspergillus; Cystic fibrosis; Sonication; Sputum

Indexed keywords

ARTICLE; ASPERGILLUS; BIOFILM; CONTROLLED STUDY; CYSTIC FIBROSIS; DNA EXTRACTION; DNA FRAGMENTATION; FUNGUS CULTURE; FUNGUS GROWTH; FUNGUS IDENTIFICATION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SPUTUM; SPUTUM ANALYSIS; ULTRASOUND;

ADULT; ASPERGILLUS; CYSTIC FIBROSIS; DNA, FUNGAL; DNA, RIBOSOMAL; HUMANS; MICROBIOLOGICAL TECHNIQUES; POLYMERASE CHAIN REACTION; PULMONARY ASPERGILLOSIS; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY; SONICATION; SPECIMEN HANDLING; SPUTUM;

ASPERGILLUS;

EID: 79952361379     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.01.024     Document Type: Article

References (28)

1. Amin R., Dupuis A., Aaron S.D., Ratjen F. The effect of chronic infection with Aspergillus fumigatus on lung function and hospitalization in cystic fibrosis patients. Chest 2010, 137(1):171-176.
2. Bakare N., Rickerts V., Bargon J., Just-Nubling G. Prevalence of Aspergillus fumigatus and other fungal species in the sputum of adult patients with cystic fibrosis. Mycoses 2003, 46(1-2):19-23.
3. Borman A.M., Palmer M.D., Delhaes L., Carrere J., Favennec L., Ranque S., Gangneux J.P., Horre R., Bouchara J.P. Lack of standardization in the procedures for mycological examination of sputum samples from CF patients: a possible cause for variations in the prevalence of filamentous fungi. Med. Mycol. 2010, 48(Suppl 1):S88-S97.
4. Denning D.W., O'Driscoll B.R., Powell G., Chew F., Atherton G.T., Vyas A., Miles J., Morris J., Niven R.M. Randomized controlled trial of oral antifungal treatment for severe asthma with fungal sensitization: the Fungal Asthma Sensitization Trial (FAST) study. Am. J. Respir. Crit. Care Med. 2009, 179(1):11-18.
5. Fairs A., Agbetile J., Hargadon B., Bourne M., Monteiro W.R., Brightling C.E., Bradding P., Green R.H., Mutalithas K., Desai D., Pavord I.D., Wardlaw A.J., Pashley C.H. IgE sensitisation to Aspergillus fumigatus is associated with reduced lung function in asthma. Am. J. Respir. Crit. Care Med. 2010, 182(11):1362-1368.
6. Fredricks D.N., Smith C., Meier A. Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR. J. Clin. Microbiol. 2005, 43(10):5122-5128.
7. Griffiths L.J., Anyim M., Doffman S.R., Wilks M., Millar M.R., Agrawal S.G. Comparison of DNA extraction methods for Aspergillus fumigatus using real-time PCR. J. Med. Microbiol. 2006, 55(Pt 9):1187-1191.
8. Guo Y.L., Chen Y.Q., Wang K., Qin S.M., Wu C., Kong J.L. Accuracy of bronchoalveolar lavage galactomannan in diagnosing invasive aspergillosis: a bivariate meta-analysis and systematic review. Chest 2010, 138(4):817-824.
9. Hamilton L.H. Errors in blood cell counting. I. Technical errors. Can. J. Med. Technol. 1955, 17(4):137-144.
10. Hamilton R., Sandin R.L. Sonication lyses cells and exposes DNA for PCR amplification: potential transfer of technology from clinical microbiology to forensic pathology. Am. J. Forensic Med. Pathol. 1999, 20(1):102-104.
11. Harris J.K., De Groote M.A., Sagel S.D., Zemanick E.T., Kapsner R., Penvari C., Kaess H., Deterding R.R., Accurso F.J., Pace N.R. Molecular identification of bacteria in bronchoalveolar lavage fluid from children with cystic fibrosis. Proc. Natl. Acad. Sci. USA 2007, 104(51):20529-20533.
12. Herrera M.L., Vallor A.C., Gelfond J.A., Patterson T.F., Wickes B.L. Strain-dependent variation in 18S ribosomal DNA copy numbers in Aspergillus fumigatus. J. Clin. Microbiol. 2009, 47(5):1325-1332.
13. Hirsch S.R., Zastrow J.E., Kory R.C. Sputum liquefying agents: a comparative in vitro evaluation. J. Lab. Clin. Med. 1969, 74(2):346-353.
14. Jewes L.A., Spencer R.C. The incidence of anaerobes in the sputum of patients with cystic fibrosis. J. Med. Microbiol. 1990, 31(4):271-274.
15. Kraemer R., Delosea N., Ballinari P., Gallati S., Crameri R. Effect of allergic bronchopulmonary aspergillosis on lung function in children with cystic fibrosis. Am. J. Respir. Crit. Care Med. 2006, 174(11):1211-1220.
16. Mengoli C., Cruciani M., Barnes R.A., Loeffler J., Donnelly J.P. Use of PCR for diagnosis of invasive aspergillosis: systematic review and meta-analysis. Lancet Infect. Dis. 2009, 9(2):89-96.
17. Nelson L.A., Callerame M.L., Schwartz R.H. Aspergillosis and atopy in cystic fibrosis. Am. Rev. Respir. Dis. 1979, 120(4):863-873.
18. Nepomuceno I.B., Esrig S., Moss R.B. Allergic bronchopulmonary aspergillosis in cystic fibrosis: role of atopy and response to itraconazole. Chest 1999, 115(2):364-370.
19. Pye A., Stockley R.A., Hill S.L. Simple method for quantifying viable bacterial numbers in sputum. J. Clin. Pathol. 1995, 48(8):719-724.
20. Randall D. Biofilms and chronic infections. JAMA 2008, 299(22):2682-2684.
21. Rogers G.B., Carroll M.P., Serisier D.J., Hockey P.M., Jones G., Bruce K.D. Characterization of bacterial community diversity in cystic fibrosis lung infections by use of 16s ribosomal DNA terminal restriction fragment length polymorphism profiling. J. Clin. Microbiol. 2004, 42(11):5176-5183.
22. Schonheyder H., Jensen T., Hoiby N., Andersen P., Koch C. Frequency of Aspergillus fumigatus isolates and antibodies to aspergillus antigens in cystic fibrosis. Acta Pathol Microbiol Immunol Scand B 1985, 93(2):105-112.
23. Shoseyov D., Brownlee K.G., Conway S.P., Kerem E. Aspergillus bronchitis in cystic fibrosis. Chest 2006, 130(1):222-226.
24. Skov M., Hoiby N., Koch C. Itraconazole treatment of allergic bronchopulmonary aspergillosis in patients with cystic fibrosis. Allergy 2002, 57(8):723-728.
25. Sparham P.D., Lobban D.I., Speller D.C. Isolation of Staphylococcus aureus from sputum in cystic fibrosis. J. Clin. Pathol. 1978, 31(10):913-918.
26. Stevens D.A., Schwartz H.J., Lee J.Y., Moskovitz B.L., Jerome D.C., Catanzaro A., Bamberger D.M., Weinmann A.J., Tuazon C.U., Judson M.A., Platts-Mills T.A., DeGraff A.C. A randomized trial of itraconazole in allergic bronchopulmonary aspergillosis. N. Engl. J. Med. 2000, 342(11):756-762.
27. Stevens D.A., Moss R.B., Kurup V.P., Knutsen A.P., Greenberger P., Judson M.A., Denning D.W., Crameri R., Brody A.S., Light M., Skov M., Maish W., Mastella G. Allergic bronchopulmonary aspergillosis in cystic fibrosis-state of the art: Cystic Fibrosis Foundation Consensus Conference. Clin. Infect. Dis. 2003, 37(Suppl 3):S225-S264.
28. Tuon F. A systematic literature review on the diagnosis of invasive aspergillosis using polymerase chain reaction (PCR) from bronchoalveolar lavage clincal samples. Rev. Iberoam. Micol. 2007, 24:89-94.