A novel technique using DNA denaturation to detect multiply induced single-strand breaks in a hydrated plasmid DNA molecule by X-ray and 4He2+ ion irradiation

Radiation Protection Dosimetry

Volumn 143, Issue 2-4, 2011, Pages 219-225

  Yokoya, Akinari ^a   Shikazono, Naoya ^a   Fujii, Kentaro ^a   Noguchi, Miho ^a   Urushibara, Ayumi ^a  

^a JAPAN ATOMIC ENERGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

HEAVY ION; HELIUM; ISOTOPE;

AGAR GEL ELECTROPHORESIS; ARTICLE; CHEMISTRY; DNA DAMAGE; DNA DENATURATION; GENETICS; METHODOLOGY; PLASMID; RADIATION DOSE; RADIATION EXPOSURE; X RAY;

DNA DAMAGE; ELECTROPHORESIS, AGAR GEL; HEAVY IONS; HELIUM; ISOTOPES; NUCLEIC ACID DENATURATION; PLASMIDS; RADIATION DOSAGE; X-RAYS;

EID: 79952358523     PISSN: 01448420     EISSN: 17423406     Source Type: Journal    
DOI: 10.1093/rpd/ncq540     Document Type: Article

References (14)

1. Nikjoo, H., O'Neill, P., Wilson, W. E. and Goodhead, D. T. Computational approach for determining the spectrum of DNA damage induced by ionizing radiation. Radiat. Res. 156, 577-583 (2001).
2. Taucher-Scholz, G. and Kraft, G. Influence of radiation quality on the yield of DNA strand breaks in SV40 DNA irradiated in solution. Radiat. Res. 151, 595-604 (1999).
3. Georgakilas, A. G., Bennett, P. V., Wilson, D. B. and Sutherland, B. M. Processing of bistranded abasic DNA clusters in γ-irradiated human hematopoietic cells. Nucleic Acids Res. 32, 5609-5620 (2004).
4. Leloup, C. et al. Evaluation of lesion clustering in irradiated plasmid DNA. Int. J. Radiat. Biol. 81, 41-54 (2005).
5. 2+ ion irradiation. Int. J. Radiat. Biol. 84, 23-33 (2008).
6. Yokoya, A., Fuji, K., Shikazono, N., Akamatsu, K., Urushibara, A. and Watanabe, R. Studies of soft X-ray-induced Auger effect on the induction of DNA damage. Int. J. Radiat. Biol. 84, 1069-1081 (2008).
7. Hanai, R., Yazu, M. and Hieda, K. On the experimental distinction between ssbs and dsbs in circular DNA. Int. J. Radiat. Biol. 73, 457-479 (1998).
8. Sharma, K. K., Milligan, J. R. and Bernhard, W. A. Multiplicity of DNA single-strand breaks produced in pUC18 exposed to the direct effects of ionizing radiation. Radiat. Res. 170, 156-162 (2008).
9. Yokoya, A., Cunniffe, C.M.T. and O'Neill, P. Effect of hydration on the induction of strand breaks and base lesions in plasmid DNA films by gamma-radiation. J. Am. Chem. Soc. 124, 8859-8866 (2002).
10. Shikazono, N., Yokoya, A., Urushibara, A., Noguchi, A. and Fujii, K. A model for analysis of the yield and the level of clustering of radiation-induced DNA strand breaks in hydrated plasmids. Radiat. Prot. Dosim. 143, 181-185 (2011).
11. Adinarayana, M., Bothe, E. and Schulte-Frohlinde, D. Hydroxyl radical-induced strand break formation in single-stranded polynucleotides and single-stranded DNA in aqueous solution as measured by light scattering and by conductivity. Int. J. Radiat. Biol. 54, 723-737 (1988).
12. von Sonntag, C. Free-radical-induced DNA Damage and its Repair-a Chemical Perspective. Springer (2006).
13. Häring, M., Rüdiger, H., Demple, B., Boiteux, S. and Epe, B. Recognition of oxidized abasic sites by repair endonucleases. Nucleic Acid Res. 22, 2010-2015 (1994).
14. Yokoya, A., Fujii, K., Shikazono, N. and Ukai, M. Chapter 21. Spectroscopic study of radiation-induced DNA lesions and their susceptibility to enzymatic repair. Charged particle and photon interactions with matterrecent advances, applications and interfaces. Hatano, Y., Katsumura, Y. and Mozumder, A., Eds. CRC/Taylor & Francis Group (in press) (2010).