Inhibition of connexin43 dephosphorylation is involved in protective effects of diltiazem on cardiac function during hypoxic injury

Histology and Histopathology

Volumn 26, Issue 3, 2011, Pages 315-322

  Matsushita, Satoshi ^a   Kurihara, Hidetake ^a   Watanabe, Makino ^b   Okada, Takao ^b   Sakai, Tatsuo ^a   Amano, Atsushi ^a  

^a Department of Cardiovascular Surgery ^*  (Japan)

^b JUNTENDO UNIVERSITY   (Japan)

Author keywords

Arrhythmia; Connexin 43; Diltiazem; Langendorff; Phosphorylation

Indexed keywords

CARDIOTONIC AGENT; CONNEXIN 43; DILTIAZEM; GAP JUNCTION PROTEIN;

ANIMAL; ANOXIA; ARTICLE; FLUORESCENT ANTIBODY TECHNIQUE; HEART; HEMODYNAMICS; IMAGE PROCESSING; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MALE; METABOLISM; PATHOPHYSIOLOGY; PERFUSION; PHOSPHORYLATION; PHYSIOLOGY; RAT; WESTERN BLOTTING; WISTAR RAT;

ANIMALS; ANOXIA; BLOTTING, WESTERN; CARDIOTONIC AGENTS; CONNEXIN 43; CONNEXINS; DILTIAZEM; FLUORESCENT ANTIBODY TECHNIQUE; HEART; HEMODYNAMICS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; MALE; PERFUSION; PHOSPHORYLATION; RATS; RATS, WISTAR;

RATTUS NORVEGICUS;

EID: 79952329055     PISSN: 02133911     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (31)

1. Ai X. and Pogwizd S.M. (2005). Connexin 43 downregulation and dephosphorylation in nonischemic heart failure is associated with enhanced colocalized protein phosphatase type 2A. Circ. Res. 96, 54-63. (Pubitemid 40095754)
2. Beardslee M.A., Lerner D.L., Tadros P.N., Laing J.G., Beyer E.C., Yamada K.A., Kléber A.G., Schuessler R.B. and Saffitz J.E. (2000). Dephosphorylation and intracellular redistribution of ventricular connexin43 during electrical uncoupling induced by ischemia. Circ. Res. 87, 656-662.
3. 2+. Am. J. Physiol. 352, C607-C612.
4. Bush L.R., Li Y.P., Shlafer M., Jolly S.R. and Lucchesi B.R. (1981). Protective effects of diltiazem during myocardial ischemia in isolated cat hearts. J. Pharmacol. Exp. Ther. 218, 653-661. (Pubitemid 11005134)
5. Giepmans B.N. and Moolenaar W.H. (1998). The gap junction protein connexin43 interacts with the second PDZ domain of the zona occludens-1 protein. Curr. Biol. 8, 931-934. (Pubitemid 28371760)
6. Gutstein D.E., Morley G.E., Tamaddon H., Vaidya D., Schneider M.D., Chen J., Chien K.R., Stuhlmann H. and Fishman G.I. (2001). Conduction slowing and sudden arrhythmic death in mice with cardiac-restricted inactivation of connexin43. Circ. Res. 88, 333-339. (Pubitemid 32173758)
7. Kihara Y. and Morgan J.P. (1991). Intracellular calcium and ventricular fibrillation. Studies in the aequorin-loaded isovolumic ferret heart. Circ. Res. 68, 1378-1389.
8. Laird D.W., Puranam K.L. and Revel J.P. (1991). Turnover and phosphorylation dynamics of connexin43 gap junction protein in cultured cardiac myocytes. Biochem. J. 273, 67-72.
9. Leykauf K., Durst M. and Alonso A. (2003). Phosphorylation and subcellular distribution of connexin43 in normal and stressed cells. Cell. Tissue Res. 311, 23-30. (Pubitemid 35478221)
10. i in perfused hearts. Critical evaluation of the 5F-BAPTA and nuclearmagnetic resonance method as applied to the study of ischemia and reperfusion. Circ. Res. 66, 1255-1267. (Pubitemid 20147742)
11. Matsushita S., Kurihara H., Watanabe M., Okada T., Sakai T. and Amano A. (2006). Alterations of phosphorylation state of connexin 43 during hypoxia and reoxygenation are associated with cardiac function. J. Histochem. Cytochem. 54, 343-353. (Pubitemid 43291279)
12. 2+ modulation?during postischemic reperfusion in rat heart. Am. J. Physiol. 268, H100-111.
13. Musil L.S. and Goodenough D.A. (1991). Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques. J. Cell. Biol. 115, 1357-1374. (Pubitemid 21909980)
14. Oelze I., Kartenbeck J., Crusius K. and Alonso A. (1995). Human papillomavirus type 16 E5 protein affects cell-cell communication in an epithelial cell line. J. Virol. 69, 4489-4494.
15. Okada T., Izawa N. and Nakamura T. (2000). Comparison of the effects of nifedipine and nisoldipine on coronary vasoconstriction in the Langendorff-perfused rat heart. J. Cardiovasc. Pharmac. 35, 145-149. (Pubitemid 30032906)
16. Opie L.H. and Coetzee W.A. (1998). Role of calcium ions in reperfusion arrhythmias: relevance to pharmacologic intervention. Cardiovasc. Drugs Ther 2, 623-636.
17. Peters N.S., Coromilas J., Severs N.J. and Wit A.L. (1997). Disturbed connexin43 gap junction distribution correlates with the location of reentrant circuits in the epicardial border zone of healing canine infarcts that cause ventricular tachycardia. Circulation 95, 988-996. (Pubitemid 27091545)
18. Reaume A.G., de Sousa P.A., Kulkarni S., Langille B.L., Zhu D., Davies T.C., Juneja S.C., Kidder G.M. and Rossant J. (1995). Cardiac malformation in neonatal mice lacking connexin43. Science 267, 1831-1834.
19. Sato R., Yamazaki J. and Nagao T. (1999). Temporal differences in actions of calcium channel blockers on K+ accumulation, cardiac function, and high-energy phosphate levels in ischemic guinea pig hearts. J. Pharmacol. Exp. Ther. 289, 831-839. (Pubitemid 29191239)
20. Schwartz A., Matlib M.A., Balwierczak J. and Lathrop D.A. (1985). Pharmacology of calcium antagonists. Am. J. Cardiol. 15, 3C-7C. (Pubitemid 15143446)
21. Smith J.H., Green C.R., Peters N.S., Rothery S. and Severs N.J. (1991). Altered patterns of gap junction distribution in ischemic heart disease. An immunohistochemical study of human myocardium using laser scanning confocal microscopy. Am. J. Pathol. 139, 801-821.
22. Steenbergen C., Murphy E., Levy L. and London R.E. (1987). Elevation in cytosolic free calcium?concentration early in myocardial ischemia in perfused rat heart. Circ. Res. 60, 700-707. (Pubitemid 17098135)
23. Takeo S., Tanonaka K., Iwai T., Motegi K. and Hirota Y. (2004). Preservation of mitochondrial function during ischemia as a possible mechanism for cardioprotection of diltiazem against ischemia/reperfusion injury. Biochem. Pharmacol. 67, 565-574. (Pubitemid 38232896)
24. TenBroek E.M., Lampe P.D., Solan J.L., Reynhout J.K. and Johnson R.G. (2001). Ser364 of connexin43 and the upregulation of gap junction assembly by cAMP. J. Cell Biol. 155, 1307-1318. (Pubitemid 34286279)
25. Toyofuku T., Yabuki M., Otsu K., Kuzuya T., Hori M. and Tada M. (1998). Direct association of the gap junction protein connexin-43 with ZO-1 in cardiac myocytes. J. Biol. Chem. 273, 12725-12731. (Pubitemid 28246824)
26. Toyofuku T., Akamatsu Y., Zhang H., Kuzuya T., Tada M. and Hori M. (2001). c-Src regulates the interaction between connexin-43 and ZO-1 in cardiac myocytes. J. Biol. Chem. 276, 1780-1788. (Pubitemid 32109650)
27. Turner M.S., Haywood G.A., Andreka P., You L., Martin P.E., Evans W.H., Webster K.A. and Bishopric N.H. (2004). Reversible connexin 43 dephosphorylation during hypoxia and reoxygenation is linked to cellular ATP levels. Circ. Res. 95, 726-733. (Pubitemid 39331933)
28. van Gilst W.H., de Graeff P.A., Kingma J.H., de Langen C.D. and Wesseling H. (1986). Effects of diltiazem on reperfusion-induced arrhythmias in vitro and in vivo. J. Mol. Cell. Cardiol. 18, 1255-66. (Pubitemid 17228944)
29. Weishaar R., Ashikawa K. and Bing R.J. (1979). Effect of diltiazem, a calcium antagonist, on myocardial ischemia. Am. J. Cardiol. 43, 1137-1143. (Pubitemid 9190995)
30. Ya J., Erdtsieck-Ernste E.B., de Boer P.A., van Kempen M.J., Jongsma H., Gros D., Moorman A.F. and Lamers W.H. (1998). Heart defects in connexin43-deficient mice. Circ. Res. 82, 360-366. (Pubitemid 28101193)
31. Zamanis A., Verdetti J. and de Leiris J. (1982). Reduction of ischemia-induced myocardial necrosis in the rat with permanent coronary artery occlusion under the effect of diltiazem. J. Mol. Cell. Cardiol. 14, 53-62. (Pubitemid 12177072)