-
1
-
-
0021818675
-
Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface
-
Smith, G. P. (1985) Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228,1315–17.
-
(1985)
Science
, vol.228
, pp. 1315-1317
-
-
Smith, G. P.1
-
2
-
-
0030974119
-
In vitro selection and evolution of functional proteins by using ribosome display
-
Hanes, J. and Plückthun, A. (1997) In vitro selection and evolution of functional proteins by using ribosome display. Proc. Natl. Acad. Sci. USA 94, 4937–42.
-
(1997)
Proc. Natl. Acad. Sci. USA
, vol.94
, pp. 4937-4942
-
-
Hanes, J.1
Plückthun, A.2
-
3
-
-
0032564346
-
Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries
-
Hanes, J., Jermutus, L., Weber-Bornhauser, S., Bosshard, H. R., and Plückthun, A. (1998) Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries. Proc. Natl. Acad. Sci. USA 95, 14130–5.
-
(1998)
Proc. Natl. Acad. Sci. USA
, vol.95
, pp. 14130-14135
-
-
Hanes, J.1
Jermutus, L.2
Weber-Bornhauser, S.3
Bosshard, H. R.4
Plückthun, A.5
-
4
-
-
0030817279
-
RNA-peptide fusions for the in vitro selection of peptides and proteins
-
Roberts, R. W. and Szostak, J. W. (1997) RNA-peptide fusions for the in vitro selection of peptides and proteins. Proc. Natl. Acad. Sci. USA 94, 12297–302.
-
(1997)
Proc. Natl. Acad. Sci. USA
, vol.94
, pp. 12297-12302
-
-
Roberts, R. W.1
Szostak, J. W.2
-
5
-
-
0033643217
-
Optimized synthesis of RNA-protein fusions for in vitro protein selection
-
Liu, R., Barrick, J. E., Szostak, J. W., and Roberts, R. W. (2000) Optimized synthesis of RNA-protein fusions for in vitro protein selection. Methods Enzymol. 318, 268–93.
-
(2000)
Methods Enzymol
, vol.318
, pp. 268-293
-
-
Liu, R.1
Barrick, J. E.2
Szostak, J. W.3
Roberts, R. W.4
-
6
-
-
0035793208
-
Stability engineering of antibody single-chain Fv fragments
-
Wörn, A. and Plückthun A. (2001) Stability engineering of antibody single-chain Fv fragments. J. Mol. Biol. 305, 989–1010.
-
(2001)
J. Mol. Biol
, vol.305
, pp. 989-1010
-
-
Wörn, A.1
Plückthun, A.2
-
7
-
-
0035793080
-
Tailoring in vitro evolution for protein affinity or stability
-
Jermutus, L., Honegger, A., Schwesinger, F., Hanes, J., and Plückthun, A. (2001) Tailoring in vitro evolution for protein affinity or stability. Proc. Natl. Acad. Sci. USA 98, 75–80.
-
(2001)
Proc. Natl. Acad. Sci. USA
, vol.98
, pp. 75-80
-
-
Jermutus, L.1
Honegger, A.2
Schwesinger, F.3
Hanes, J.4
Plückthun, A.5
-
8
-
-
0028050350
-
Rapid evolution of a protein in vitro by DNA shuffling
-
Stemmer, W. P. (1994) Rapid evolution of a protein in vitro by DNA shuffling. Nature 370, 389–91.
-
(1994)
Nature
, vol.370
, pp. 389-391
-
-
Stemmer, W. P.1
-
9
-
-
0029869449
-
An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues
-
Zaccolo, M., Williams, D. M., Brown, D. M., and Gherardi, E. (1996) An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues. J. Mol. Biol. 255, 589–603.
-
(1996)
J. Mol. Biol
, vol.255
, pp. 589-603
-
-
Zaccolo, M.1
Williams, D. M.2
Brown, D. M.3
Gherardi, E.4
-
10
-
-
0028592703
-
An in vitro polysome display system for identifying ligands from very large peptide libraries
-
Mattheakis, L. C., Bhatt, R. R., and Dower, W. J. (1994) An in vitro polysome display system for identifying ligands from very large peptide libraries. Proc. Natl. Acad. Sci. USA 91, 9022–6.
-
(1994)
Proc. Natl. Acad. Sci. USA
, vol.91
, pp. 9022-9026
-
-
Mattheakis, L. C.1
Bhatt, R. R.2
Dower, W. J.3
-
11
-
-
0033733267
-
Selecting and evolving functional proteins in vitro by ribosome display
-
Hanes, J., Jermutus, L., and Plückthun, A. (2000) Selecting and evolving functional proteins in vitro by ribosome display. Methods Enzymol. 328, 404–30.
-
(2000)
Methods Enzymol
, vol.328
, pp. 404-430
-
-
Hanes, J.1
Jermutus, L.2
Plückthun, A.3
-
12
-
-
0033664270
-
Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display
-
Hanes, J., Schaffitzel, C., Knappik, A., and Plückthun, A. (2000) Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display. Nat. Biotechnol. 18, 1287–92.
-
(2000)
Nat. Biotechnol
, vol.18
, pp. 1287-1292
-
-
Hanes, J.1
Schaffitzel, C.2
Knappik, A.3
Plückthun, A.4
-
13
-
-
33748793940
-
Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation
-
Luginbühl, B., Kanyo, Z., Jones, R. M., Fletterick, R. J., Prusiner, S. B., Cohen, F. E., Williamson, R. A., Burton, D. R., and Plückthun, A. (2006) Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation. J. Mol. Biol. 363, 75–97.
-
(2006)
J. Mol. Biol
, vol.363
, pp. 75-97
-
-
Luginbühl, B.1
Kanyo, Z.2
Jones, R. M.3
Fletterick, R. J.4
Prusiner, S. B.5
Cohen, F. E.6
Williamson, R. A.7
Burton, D. R.8
Plückthun, A.9
-
14
-
-
0042780350
-
Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins
-
Binz, H. K., Stumpp, M. T., Forrer, P., Amstutz, P., and Plückthun, A. (2003) Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins. J. Mol. Biol. 332, 489–503.
-
(2003)
J. Mol. Biol
, vol.332
, pp. 489-503
-
-
Binz, H. K.1
Stumpp, M. T.2
Forrer, P.3
Amstutz, P.4
Plückthun, A.5
-
15
-
-
34248549239
-
A designed ankyrin repeat protein evolved to picomolar affinity to Her2
-
Zahnd, C., Wyler, E., Schwenk, J. M., Steiner, D., Lawrence, M. C., McKern, N. M., Pecorari, F., Ward, C. W., Joos, T. O., and Plückthun, A. (2007) A designed ankyrin repeat protein evolved to picomolar affinity to Her2. J. Mol. Biol. 369, 1015–28.
-
(2007)
J. Mol. Biol
, vol.369
, pp. 1015-1028
-
-
Zahnd, C.1
Wyler, E.2
Schwenk, J. M.3
Steiner, D.4
Lawrence, M. C.5
McKern, N. M.6
Pecorari, F.7
Ward, C. W.8
Joos, T. O.9
Plückthun, A.10
-
16
-
-
2342624119
-
High-affinity binders selected from designed ankyrin repeat protein libraries
-
Binz, H. K., Amstutz, P., Kohl, A., Stumpp, M. T., Briand, C., Forrer, P., Grütter, M. G., and Plückthun, A. (2004) High-affinity binders selected from designed ankyrin repeat protein libraries. Nat. Biotechnol. 22, 575–82.
-
(2004)
Nat. Biotechnol
, vol.22
, pp. 575-582
-
-
Binz, H. K.1
Amstutz, P.2
Kohl, A.3
Stumpp, M. T.4
Briand, C.5
Forrer, P.6
Grütter, M. G.7
Plückthun, A.8
-
17
-
-
21744440783
-
Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins
-
Amstutz, P., Binz, H. K., Parizek, P., Stumpp, M. T., Kohl, A., Grütter, M. G., Forrer, P., and Plückthun, A. (2005) Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins. J. Biol. Chem. 280, 24715–22.
-
(2005)
J. Biol. Chem
, vol.280
, pp. 24715-24722
-
-
Amstutz, P.1
Binz, H. K.2
Parizek, P.3
Stumpp, M. T.4
Kohl, A.5
Grütter, M. G.6
Forrer, P.7
Plückthun, A.8
-
18
-
-
33845939857
-
Selection and characterization of Her2 binding-designed ankyrin repeat proteins
-
Zahnd, C., Pécorari, F., Straumann, N., Wyler, E., and Plückthun, A. (2006) Selection and characterization of Her2 binding-designed ankyrin repeat proteins. J. Biol. Chem. 281, 35167–75.
-
(2006)
J. Biol. Chem
, vol.281
, pp. 35167-35175
-
-
Zahnd, C.1
Pécorari, F.2
Straumann, N.3
Wyler, E.4
Plückthun, A.5
-
19
-
-
34247853389
-
Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism
-
Schweizer, A., Roschitzki-Voser, H., Amstutz, P., Briand, C., Gulotti-Georgieva, M., Prenosil, E., Binz, H. K., Capitani, G., Baici, A., Plückthun, A., and Grütter, M. G. (2007) Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism. Structure 15, 625–36.
-
(2007)
Structure
, vol.15
, pp. 625-636
-
-
Schweizer, A.1
Roschitzki-Voser, H.2
Amstutz, P.3
Briand, C.4
Gulotti-Georgieva, M.5
Prenosil, E.6
Binz, H. K.7
Capitani, G.8
Baici, A.9
Plückthun, A.10
Grütter, M. G.11
-
21
-
-
0039310043
-
Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli
-
Schatz, P. J. (1993) Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli. Biotechnology 11, 101138–43.
-
(1993)
Biotechnology
, vol.11
, pp. 101138-101143
-
-
Schatz, P. J.1
-
22
-
-
0013967653
-
Magnesium ion-independent ribonucleic acid depolymerases in bacteria
-
Wade, H. E. and Robinson, H. K. (1966) Magnesium ion-independent ribonucleic acid depolymerases in bacteria. Biochem. J. 101, 467–79.
-
(1966)
Biochem. J
, vol.101
, pp. 467-479
-
-
Wade, H. E.1
Robinson, H. K.2
-
23
-
-
0024363590
-
The precursor of beta-lactamase: purification, properties and folding kinetics
-
Laminet, A. A. and Plückthun, A. (1989) The precursor of beta-lactamase: purification, properties and folding kinetics. EMBO J. 8, 1469–77.
-
(1989)
EMBO J
, vol.8
, pp. 1469-1477
-
-
Laminet, A. A.1
Plückthun, A.2
-
24
-
-
0015327122
-
Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate
-
O’Callaghan, C. H., Morris, A., Kirby, S. M., and Shingler, A. H. (1972) Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob. Agents Chemother. 1, 283–8.
-
(1972)
Antimicrob. Agents Chemother
, vol.1
, pp. 283-288
-
-
O’Callaghan, C. H.1
Morris, A.2
Kirby, S. M.3
Shingler, A. H.4
-
25
-
-
51349091340
-
Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display
-
Steiner, D., Forrer, P., and Plückthun, A. (2008) Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display. J. Mol. Biol. 382, 1211–27.
-
(2008)
J. Mol. Biol
, vol.382
, pp. 1211-1227
-
-
Steiner, D.1
Forrer, P.2
Plückthun, A.3
-
26
-
-
33847634290
-
Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target
-
Zahnd, C., Amstutz, P., and Plückthun, A. (2007) Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target. Nat. Methods 4, 269–79.
-
(2007)
Nat. Methods
, vol.4
, pp. 269-279
-
-
Zahnd, C.1
Amstutz, P.2
Plückthun, A.3
-
27
-
-
0029825005
-
Multiple degradation pathways of the rpsO mRNA of Escherichia coli. RNase E interacts with the 5¢ and 3¢ extremities of the primary transcript
-
Hajnsdorf, E., Braun, F., Haugel-Nielsen, J., Le Derout, J., and Régnier, P. (1996) Multiple degradation pathways of the rpsO mRNA of Escherichia coli. RNase E interacts with the 5¢ and 3¢ extremities of the primary transcript. Biochimie 78, 416–24.
-
(1996)
Biochimie
, vol.78
, pp. 416-424
-
-
Hajnsdorf, E.1
Braun, F.2
Haugel-Nielsen, J.3
Le Derout, J.4
Régnier, P.5
-
28
-
-
0026699293
-
Selection of phage antibodies by binding affinity. Mimicking affinity maturation
-
Hawkins, R. E., Russell, S. J., and Winter, G. (1992) Selection of phage antibodies by binding affinity. Mimicking affinity maturation. J. Mol. Biol. 226, 889–96.
-
(1992)
J. Mol. Biol
, vol.226
, pp. 889-896
-
-
Hawkins, R. E.1
Russell, S. J.2
Winter, G.3
-
29
-
-
0029564921
-
CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range
-
Yang, W. P., Green, K., Pinz-Sweeney, S., Briones, A. T., Burton, D. R., and Barbas, C. F. 3rd. (1995) CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range. J. Mol. Biol. 254, 392–403.
-
(1995)
J. Mol. Biol
, vol.254
, pp. 392-403
-
-
Yang, W. P.1
Green, K.2
Pinz-Sweeney, S.3
Briones, A. T.4
Burton, D. R.5
Barbas, C. F.6
-
30
-
-
84884900154
-
Ribosome display: In vitro selection of protein-protein interactions
-
(Celis, J., ed), Elsevier, Amsterdam
-
Amstutz, P., Binz, H. K., Zahnd, C., and Plückthun, A. (2006) Ribosome display: In vitro selection of protein-protein interactions. In “Cell Biology: A Laboratory Handbook” (Celis, J., ed.), 497–509. Elsevier, Amsterdam.
-
(2006)
Cell Biology: A Laboratory Handbook
, pp. 497-509
-
-
Amstutz, P.1
Binz, H. K.2
Zahnd, C.3
Plückthun, A.4
-
31
-
-
0020645081
-
Prokaryotic coupled transcription-translation
-
Chen, H. Z. and Zubay, G. (1983) Prokaryotic coupled transcription-translation. Methods Enzymol. 101, 674–690.
-
(1983)
Methods Enzymol
, vol.101
, pp. 674-690
-
-
Chen, H. Z.1
Zubay, G.2
-
32
-
-
0001951544
-
Coupled transcription-translation in prokaryotic cell-free systems
-
(Hemes B. D. and Higgins S. J., eds), IRL Press, Oxford
-
Pratt, J. M. (1984) Coupled transcription-translation in prokaryotic cell-free systems. In “Current Protocols” (Hemes B. D. and Higgins S. J., eds.), 179–209. IRL Press, Oxford.
-
(1984)
Current Protocols
, pp. 179-209
-
-
Pratt, J. M.1
-
33
-
-
0036719292
-
mRNA decay in Escherichia coli comes of age
-
discussion 4657
-
Kushner, S. R. (2002) mRNA decay in Escherichia coli comes of age. J. Bacteriol. 184, 4658–65; discussion 4657.
-
(2002)
J. Bacteriol
, vol.184
, pp. 4658-4665
-
-
Kushner, S. R.1
-
35
-
-
77954636166
-
Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution
-
Zahnd, C., Sarkar, C. A., and Plückthun, A. (2010) Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution. Protein Eng. Des. Sel. 23, 175–84.
-
(2010)
Protein Eng. Des. Sel
, vol.23
, pp. 175-184
-
-
Zahnd, C.1
Sarkar, C. A.2
Plückthun, A.3
-
36
-
-
0033571383
-
High thermal stability is essential for tumor targeting of antibody fragments: Engineering of a humanized anti-epithelial glycoprotein-2 (epi-thelial cell adhesion molecule) single-chain Fv fragment
-
Willuda, J., Honegger, A., Waibel, R., Schubiger, P. A., Stahel, R., Zangemeister-Wittke, U., and Plückthun, A. (1999) High thermal stability is essential for tumor targeting of antibody fragments: Engineering of a humanized anti-epithelial glycoprotein-2 (epi-thelial cell adhesion molecule) single-chain Fv fragment. Cancer Res. 59, 5758–67.
-
(1999)
Cancer Res
, vol.59
, pp. 5758-5767
-
-
Willuda, J.1
Honegger, A.2
Waibel, R.3
Schubiger, P. A.4
Stahel, R.5
Zangemeister-Wittke, U.6
Plückthun, A.7
-
37
-
-
0344813702
-
Antibody scFv fragments without disulfide bonds made by molecular evolution
-
Proba, K., Wörn, A., Honegger, A., and Plückthun, A. (1998) Antibody scFv fragments without disulfide bonds made by molecular evolution. J. Mol. Biol. 275, 245–53.
-
(1998)
J. Mol. Biol
, vol.275
, pp. 245-253
-
-
Proba, K.1
Wörn, A.2
Honegger, A.3
Plückthun, A.4
-
38
-
-
0034256057
-
Antibody engineering and its applications in tumor targeting and intracellular immunization
-
Chames, P. and Baty D. (2000) Antibody engineering and its applications in tumor targeting and intracellular immunization. FEMS Microbiol. Lett. 189, 1–8.
-
(2000)
FEMS Microbiol. Lett
, vol.189
, pp. 1-8
-
-
Chames, P.1
Baty, D.2
-
39
-
-
60749123075
-
The influence of the framework core residues on the biophysical properties of immunoglob-ulin heavy chain variable domains
-
Honegger, A., Malebranche, A. D., Röthlisberger, D., and Plückthun, A. (2009) The influence of the framework core residues on the biophysical properties of immunoglob-ulin heavy chain variable domains. Protein Eng. Des. Sel. 22, 121–34.
-
(2009)
Protein Eng. Des. Sel
, vol.22
, pp. 121-134
-
-
Honegger, A.1
Malebranche, A. D.2
Röthlisberger, D.3
Plückthun, A.4
|