Analysis of fluorescence lifetime imaging microscopy (FLIM) data: Based on a fully automated laguerre deconvolution method

Methods of Information in Medicine

Volumn 49, Issue 5, 2010, Pages 531-536

  Pande, P ^a   Trivedi, C A ^a   Jo, J A ^a  

^a Texas A M University   (United States)

Author keywords

Fluorescence lifetime imaging microscopy; Laguerre deconvolution; Nonlinear least squares; Optimization

Indexed keywords

COLLAGEN; ELASTIN;

ARTERY; ARTICLE; ATHEROSCLEROTIC PLAQUE; CADAVER; CHEMISTRY; CYTOLOGY; EVALUATION; FLUORESCENCE MICROSCOPY; HUMAN; IMAGE PROCESSING; METHODOLOGY; PATHOLOGY; REGRESSION ANALYSIS; STATISTICAL ANALYSIS; STATISTICAL MODEL; VALIDATION STUDY;

ARTERIES; COLLAGEN; DATA INTERPRETATION, STATISTICAL; ELASTIN; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LEAST-SQUARES ANALYSIS; LINEAR MODELS; MICROSCOPY, FLUORESCENCE; PLAQUE, ATHEROSCLEROTIC; POSTMORTEM CHANGES;

EID: 79952277241     PISSN: 00261270     EISSN: None     Source Type: Journal    
DOI: 10.3414/ME09-02-0046     Document Type: Article

References (7)

1. Ramanujan VK, Jo JA, Cantu G, Herman BA. Spatially resolved fluorescence lifetime mapping of enzyme kinetics in living cells. J Microsc 2008; 230 (Pt 3): 329-338.
2. Marmarelis VZ. Identification of nonlinear biological systems using Laguerre expansions of kernels. Ann Biomed Eng 1993; 21 (6): 573-589.
3. Dabir AS, Trivedi CA, Ryu Y, Pande P, Jo JA. Fully automated deconvolution method for on-line analysis of time-resolved fluorescence spectroscopy data based on an iterative Laguerre expansion technique. J Biomed Opt 2009; 14 (2): 024030.
4. Lakowicz J. Principles of Fluorescence Spectros-copy. Springer; 2006.
5. Fang QY, Papaioannou T, Jo JA, Vaitha R, Shastry K, Marcu L. Time-domain laser-induced fluorescence spectroscopy apparatus for clinical diagnostics. Review of Scientific Instruments 2004; 75 (1): 151-162.
6. Arakawa K, Isoda K, Ito T, Nakajima K, Shibuya T, Ohsuzu F. Fluorescence analysis of biochemical constituents identifies atherosclerotic plaque with a thin fibrous cap. Arterioscler Thromb Vasc Biol 2002; 22 (6): 1002-1007.
7. Marcu L, Jo JA, Fang Q, Papaioannou T, Reil T, Qiao JH, et al. Detection of rupture-prone atherosclerotic plaques by time-resolved laser-induced fluorescence spectroscopy. Atherosclerosis 2009; 204 (1): 156-164.