Generation of acetyllysine antibodies and affinity enrichment of acetylated peptides

Nature Protocols

Volumn 5, Issue 9, 2010, Pages 1583-1595

  Guan, Kun Liang ^a,b   Yu, Wei ^a   Lin, Yan ^a   Xiong, Yue ^a,c   Zhao, Shimin ^a  

^a FUDAN UNIVERSITY   (China)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; ARGININOSUCCINATE LYASE; LYSINE; OVALBUMIN; PEPTIDE;

ACETYLATION; AFFINITY CHROMATOGRAPHY; ARTICLE; CELL FRACTIONATION; CHEMISTRY; HUMAN; IMMUNOLOGICAL PROCEDURES; ISOLATION AND PURIFICATION; LIVER; METABOLISM; METHODOLOGY; PHYSIOLOGY; PROTEIN PROCESSING;

ACETYLATION; ANTIBODIES; ARGININOSUCCINATE LYASE; CHROMATOGRAPHY, AFFINITY; HUMANS; IMMUNOLOGIC TECHNIQUES; LIVER; LYSINE; OVALBUMIN; PEPTIDES; PROTEIN PROCESSING, POST-TRANSLATIONAL; SUBCELLULAR FRACTIONS;

EID: 79952276306     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.117     Document Type: Article

References (17)

1. Driessen, H.P., de Jong, W.W., Tesser, G.I., Bloemendal, H., The mechanism of N-terminal acetylation of proteins. CRC Crit. Rev. Biochem.18, 281-325 (1985).
2. Yang, X.J., Seto, E., HATs and HDACs: from structure, function and regulation to novel strategies for therapy and prevention. Oncogene 26, 5310-5318 (2007).
3. Phillips, D.M., The presence of acetyl groups of histones. Biochem. J. 87, 258-263 (1963).
4. Allfrey, V.G., Faulkner, R., Mirsky, A.E., Acetylation and methylation of histones and their possible role in the regulation of RNA synthesis. Proc. Natl. Acad. Sci. USA 51, 786-794 (1964).
5. Grunstein, M., Histone acetylation in chromatin structure and transcription. Nature 389, 349-352 (1997).
6. Paolinelli, R., Mendoza-Maldonado, R., Cereseto, A., Giacca, M., Acetylation by GCN5 regulates CDC6 phosphorylation in the S phase of the cell cycle. Nat. Struct. Mol. Biol. 16, 412-420 (2009).
7. Gu, W., Roeder, R.G., Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain. Cell 90, 595-606 (1997).
8. Marzio, G. et al. E2F family members are differentially regulated by reversible acetylation. J. Biol. Chem. 275, 10887-10892 (2000).
9. Yang, Y. et al. Acetylation of FoxO1 activates Bim expression to induce apoptosis in response to histone deacetylase inhibitor depsipeptide treatment. Neoplasia 11, 313-324 (2009).
10. Kim, S.C. et al.Substrate and functional diversity of lysine acetylation revealed by a proteomics survey. Mol. Cell 23, 607-618 (2006).
11. Zhao, S. et al. Regulation of cellular metabolism by protein lysine acetylation. Science 327, 1000-1004 (2006).
12. Choudhary, C. et al. Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science 325, 834-840 (2009).
13. Qiang, L., Xiao, H., Campos, E.I., Ho, V.C., Li, G., Development of a PAN-specific, affinity-purified anti-acetylated lysine antibody for detection, identification, isolation, and intracellular localization of acetylated protein. J. Immunoassay Immunochem. 26, 13-23 (2005).
14. Hirschey, M.D., Shimazu, T., Huang, J.Y., Verdin, E. Acetylation of mitochondrial proteins. Methods Enzymol. 457, 137-147 (2009).
15. Takemura, R. et al. Increased microtubule stability and alpha tubulin acetylation in cells transfected with microtubule-associated proteins MAP1B, MAP2 or tau. J. Cell Sci. 103(Pt 4): 953-964 (1992).
16. Gaertig, J. et al. Acetylation of lysine 40 in alpha-tubulin is not essential in Tetrahymena thermophila. J. Cell Biol. 129, 1301-1310 (1995).
17. Lu, B., Iwuoha, E.I., Smyth, M.R., O’Kennedy, R., Effects of acetonitrileon horseradish peroxidase (HRP)-anti HRP antibody interaction. Biosens. Bioelectron. 12, 619-625 (1997)