Strategy for purification and mass spectrometry identification of SELDI peaks corresponding to low-abundance plasma and serum proteins

Journal of Proteomics

Volumn 74, Issue 4, 2011, Pages 420-430

  Fertin, Marie ^a,b,c,d   Burdese, Justine ^a,b,c,d   Beseme, Olivia ^a,b,c   Amouyel, Philippe ^a,b,c,d   Bauters, Christophe ^a,b,c,d   Pinet, Florence ^a,b,c,d  

^a INSERM   (France)

^b INSTITUT PASTEUR DE LILLE   (France)

^c UNIV LILLE   (France)

^d LILLE UNIVERSITY HOSPITAL   (France)

Author keywords

Chromatographic column; Combinatorial peptide ligand library; Liquid phase isoelectric focusing; Plasma; Protein purification; SELDI TOF MS; Serum

Indexed keywords

PLASMA PROTEIN;

ARTICLE; CLINICAL ARTICLE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; GEL ELECTROPHORESIS; HUMAN; ISOELECTRIC FOCUSING; LIQUID PHASE MICROEXTRACTION; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN PURIFICATION; REVERSED PHASE LIQUID CHROMATOGRAPHY; SURFACE ENHANCED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY;

ALGORITHMS; AMINO ACID SEQUENCE; BIOLOGICAL MARKERS; BLOOD PROTEINS; EFFICIENCY; HUMANS; MASS SPECTROMETRY; MODELS, BIOLOGICAL; MYOCARDIAL INFARCTION; OSMOLAR CONCENTRATION; PLASMA; PROGNOSIS; PROTEOMICS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 79952251806     PISSN: 18743919     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jprot.2010.12.005     Document Type: Article

References (16)

1. Righetti P.G., Boschetti E., Lomas L., Citterio A. Protein Equalizer Technology: the quest for a "democratic proteome". Proteomics 2006, 6:3980-3992.
2. Guerrier L., Thulasiraman V., Castagna A., Fortis F., Lin S., Lomas L., et al. Reducing protein concentration range of biological samples using solid-phase ligand libraries. J Chromatogr B Analyt Technol Biomed Life Sci 2006, 833:33-40.
3. Thulasiraman V., Lin S., Gheorghiu L., Lathrop J., Lomas L., Hammond D., et al. Reduction of the concentration difference of proteins in biological liquids using a library of combinatorial ligands. Electrophoresis 2005, 26:3561-3571.
4. Guerrier L., Righetti P.G., Boschetti E. Reduction of dynamic protein concentration range of biological extracts for the discovery of low-abundance proteins by means of hexapeptide ligand library. Nat Protoc 2008, 3:883-890.
5. Dwivedi R.C., Krokhin O.V., Cortens J.P., Wilkins J.A. Assessment of the reproducibility of random hexapeptide peptide library-based protein normalization. J Proteome Res 2010, 9:1144-1149.
6. Fertin M., Beseme O., Duban S., Amouyel P., Bauters C., Pinet F. Deep plasma proteomic analysis of patients with left ventricular remodeling after a first myocardial infarction. Proteomics Clin Appl 2010, 4:654-673.
7. Beseme O., Fertin M., Drobecq H., Amouyel P., Pinet F. Combinatorial peptide ligand library plasma treatment: advantages for accessing low-abundance proteins. Electrophoresis 2010, 31:2697-2704.
8. Reddy G., Dalmasso E.A. SELDI ProteinChip(R) array technology: protein-based predictive medicine and drug discovery applications. J Biomed Biotechnol 2003, 2003:237-241.
9. Issaq H.J., Conrads T.P., Prieto D.A., Tirumalai R., Veenstra T.D. SELDI-TOF MS for diagnostic proteomics. Anal Chem 2003, 75:148A-155A.
10. Belluco C., Petricoin E.F., Mammano E., Facchiano F., Ross-Rucker S., Nitti D., et al. Serum proteomic analysis identifies a highly sensitive and specific discriminatory pattern in stage 1 breast cancer. Ann Surg Oncol 2007, 14:2470-2476.
11. Fan Y., Wang J., Yang Y., Liu Q., Yu J., Zheng S., et al. Detection and identification of potential biomarkers of breast cancer. J Cancer Res Clin Oncol 2010, 136:1243-1254.
12. Yang Y., Zhao S., Fan Y., Zhao F., Liu Q., Hu W., et al. Detection and identification of potential biomarkers of non-small cell lung cancer. Technol Cancer Res Treat 2009, 8:455-466.
13. Shintani S., Hamakawa H., Ueyama Y., Hatori M., Toyoshima T. Identification of a truncated cystatin SA-I as a saliva biomarker for oral squamous cell carcinoma using the SELDI ProteinChip platform. Int J Oral Maxillofac Surg 2010, 39:68-74.
14. Pinet F., Beseme O., Cieniewski-Bernard C., Drobecq H., Jourdain S., Lamblin N., et al. Predicting left ventricular remodeling after a first myocardial infarction by plasma proteome analysis. Proteomics 2008, 8:1798-1808.
15. Hey J., Posch A., Cohen A., Liu N., Harbers A. Fractionation of complex protein mixtures by liquid-phase isoelectric focusing. Meth Mol Biol 2008, 424:225-239.
16. Neuhoff V., Arold N., Taube D., Ehrhardt W. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 1988, 9:255-262.