Detection and elimination of interference by the heterophilic antibody in antibody microarray-based immunoassay

Clinical Chemistry

Volumn 57, Issue 3, 2011, Pages 527-529

  Yang, Xiaoli ^a,b   Ji, Haipeng ^b   Lin, Xiaolin ^b   Han, Zhijun ^a   Liu, Feizhou ^b   Zhong, Renqian ^a  

^a SECOND MILITARY MEDICAL UNIVERSITY   (China)

^b Shanghai Branch   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY;

BLOOD SAMPLING; HUMAN; IMMUNOASSAY; LETTER; MEASUREMENT; MICROARRAY ANALYSIS; QUANTITATIVE ANALYSIS;

ANTIBODIES, HETEROPHILE; ANTIBODIES, IMMOBILIZED; ANTIBODIES, MONOCLONAL; HUMANS; IMMUNOASSAY; PROTEIN ARRAY ANALYSIS; SENSITIVITY AND SPECIFICITY; TUMOR MARKERS, BIOLOGICAL;

EID: 79952233606     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2010.156448     Document Type: Letter

References (5)

1. Ellington AA, Kullo IJ, Bailey KR, Klee GG. Antibody-based protein multiplex platforms: technical and operational challenges. Clin Chem 2010;56:186-93.
2. Boscato LM, Stuart MC. Heterophilic antibodies: a problem for all immunoassays. Clin Chem 1988;34:27-33. (Pubitemid 18039404)
3. Kuroki M, Matsumoto Y, Arakawa F, Haruno M, Murakami M, Kuwahara M, et al. Reducing interference from heterophilic antibodies in a two-site immunoassay for carcinoembryonic antigen (CEA) by using a human/mouse chimeric antibody to CEA as the tracer. J Immunol Methods 1995;180:81-91.
4. Butler SA, Cole LA. Use of heterophilic antibody blocking agent (HBT) in reducing false-positive hCG results. Clin Chem 2001;47:1332-3. (Pubitemid 32579135)
5. Levit-Binnun N, Lindner AB, Zik O, Eshhar Z, Moses E. Quantitative detection of protein arrays. Anal Chem 2003;75:1436-41. (Pubitemid 36397178)