Optical scatter changes at the onset of apoptosis are spatially associated with mitochondria

Journal of Biomedical Optics

Volumn 15, Issue 4, 2010, Pages

  Pasternack, Robert M ^a   Zheng, Jing Yi ^a   Boustany, Nada N ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

Biology; Cells; Fluorescence; Microscopy; Scattering

Indexed keywords

DYNAMIC CHANGES; FLUORESCENCE IMAGING; INTENSITY RATIO; MITOCHONDRIAL FRAGMENTATION; OPTICAL SCATTER; OPTICAL SCATTER IMAGING; SPATIAL RELATIONSHIPS; SUB-CELLULAR;

CELL DEATH; ENDOTHELIAL CELLS; FLUORESCENCE;

MITOCHONDRIA;

ANIMAL; APOPTOSIS; CATTLE; CELL CULTURE; COMPUTER ASSISTED DIAGNOSIS; ENDOTHELIUM CELL; FLUORESCENCE MICROSCOPY; LETTER; LIGHT; METHODOLOGY; MITOCHONDRION; PHOTOMETRY; PHYSIOLOGY; RADIATION SCATTERING; REFRACTOMETRY; ULTRASTRUCTURE;

ANIMALS; APOPTOSIS; CATTLE; CELLS, CULTURED; ENDOTHELIAL CELLS; IMAGE INTERPRETATION, COMPUTER-ASSISTED; LIGHT; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; NEPHELOMETRY AND TURBIDIMETRY; REFRACTOMETRY; SCATTERING, RADIATION;

EID: 79952202147     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.3467501     Document Type: Article

References (13)

1. N. N. Danial and S. J. Korsmeyer, "Cell death: critical control points," Cell 116, 205-219 (2004). (Pubitemid 38167313)
2. D.-F. Suen, K. L. Norris, and R. J. Youle, "Mitochondrial dynamics and apoptosis," Genes Dev. 22, 1577-1590 (2008). (Pubitemid 351846838)
3. J. D. Wilson, W. J. Cottrell, and T. H. Foster, "Index-of- refractiondependent subcellular light scattering observed with organellespecific dyes," J. Biomed. Opt. 12(1), 014010 (2007).
4. N. N. Boustany, Y. C. Tsai, B. Pfister, W. M. Joiner, G. A. Oyler, and N. V. Thakor, "BCL-x(L)-dependent light scattering by apoptotic cells," Biophys. J. 87(6), 4163-4171 (2004). (Pubitemid 39602920)
5. J. D. Wilson, B. R. Giesselman, S. Mitra, and T. H. Foster, "Lysosome-damage-induced scattering changes coincide with release of cytochrome c," Opt. Lett. 32(17), 2517-2519 (2007). (Pubitemid 351302971)
6. K. J. Chalut, J. H. Ostrander, M. G. Giacomelli, and A. Wax, "Light scattering measurements of subcellular structure provide noninvasive early detection of chemotherapy-induced apoptosis," Cancer Res. 69(3), 1199-1204 (2009).
7. N. N. Boustany, R. Drezek, and N. V. Thakor, "Calcium-induced alterations in mitochondrial morphology quantified in situ with optical scatter imaging," Biophys. J. 83, 1691-1700 (2002). (Pubitemid 34977739)
8. J. Y. Zheng, R. M. Pasternack, and N. N. Boustany, "Optical scatter imaging with a digital micromirror device," Opt. Express 17(22), 20401-20414 (2009).
9. N. N. Boustany, S. C. Kuo, and N. V. Thakor, "Optical scatter imaging: subcellular morphometry in situ with Fourier filtering," Opt. Lett. 26(14), 1063-1065 (2001). (Pubitemid 33694749)
10. D. Dunn, W. E. Higgins, and J. Wakeley, "Texture segmentation using 2-D Gabor elementary functions," IEEE Trans. Pattern Anal. Mach. Intell. 16(2), 130-149 (1994).
11. A. V. Loud, "A quantitative stereological description of the ultrastructure of normal liver parenchymal cells," J. Cell Biol. 37, 27-46 (1968).
12. R. M. Pasternack, J. Zheng, and N. N. Boustany, "Quantifying mitochondrial dynamics in apoptotic cells with optical Gabor-like filtering," in Biomedical Optics, OSA Technical Digest (CD), paper BTuE3, Optical Society of America, Washington, DC (2010).
13. J. Zheng, Y. Tsai, P. Kadimcherla, R. Zhang, J. Shi, G. A. Oyler, and N. N. Boustany, "The C-terminal transmembrane domain of Bcl-xL mediates changes in mitochondrial morphology," Biophys. J. 94(1), 286-297 (2008).