Enzyme Nanoparticle Fabrication: Magnetic Nanoparticle Synthesis and Enzyme Immobilization

Methods in Molecular Biology

Volumn 679, Issue , 2011, Pages 183-191

  Johnson, Patrick A ^a   Park, Hee Joon ^a   Driscoll, Ashley J ^a  

^a UNIVERSITY OF WYOMING   (United States)

Author keywords

Biocatalyst; Enzyme immobilization; Enzyme recycling; Magnetic nanoparticles

Indexed keywords

CROSS LINKING REAGENT; ENZYME; GLUTARALDEHYDE; IMMOBILIZED ENZYME; NANOPARTICLE;

ARTICLE; BIOTECHNOLOGY; CATALYSIS; CHEMISTRY; MAGNETISM; METHODOLOGY; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

BIOTECHNOLOGY; CATALYSIS; CROSS-LINKING REAGENTS; ENZYMES; ENZYMES, IMMOBILIZED; GLUTARAL; MAGNETICS; MICROSCOPY, ELECTRON, TRANSMISSION; NANOPARTICLES;

MLCS; MLOWN;

EID: 79952197637     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-895-9_15     Document Type: Chapter

References (16)

1. Bornscheuer, U. T. (2003) Immobilizing enzymes: how to create more suitable biocatalysts. Angewandte Chemie International Edition 42, 3336–3337.
2. Sheldon, R. A., Schoevaart, R., and Van Langen, L. M. (2005) Cross-linked enzyme aggregates (CLEAs): A novel and versatile method for enzyme immobilization (a review). Biocatalysis & Biotransformation 23, 141–147.
3. Kouassi, G., Irudayaraj, J., and McCarty, G. (2005) Activity of glucose oxidase functionalized onto magnetic nanoparticles. BioMagnetic Research and Technology 3, 1.
4. Liao, M., and Chen, D. (2001) Immobilization of yeast alcohol dehydrogenase on magnetic nanoparticles for improving its stability. Biotechnology Letters 23, 1723–1727.
5. Huang, S., Liao, M., and Chen, D. (2003) Direct binding and characterization of lipase onto magnetic nanoparticles. Biotechnology Progress 19, 1095–1100.
6. Koneracká, M., Kopcanský, P., Antalík, M., Timko, M., Ramchand, C. N., Lobo, D., Mehta, R. V., and Upadhyay, R. V. (1999) Immobilization of proteins and enzymes to fine magnetic particles. Journal of Magnetism and Magnetic Materials 201, 427–430.
7. Pankhurst, Q. A., Connolly, J., Jones, S. K., and Dobson, J. (2003) Applications of magnetic nanoparticles in biomedicine. Journal of Physics D: Applied Physics 36, R167–R181.
8. Tartaj, P., Morales, M. D. P., Veintemillas-Verdaguer, S., Gonzalez-Carreno, T., and Serna, C. J. (2003) The preparation of magnetic nanoparticles for applications in biomedicine. Journal of Physics D: Applied Physics 36, R182–R197.
9. Zeng, L., Luo, K., and Gong, Y. (2006) Preparation and characterization of dendritic composite magnetic particles as a novel enzyme immobilization carrier. Journal of Molecular Catalysis B: Enzymatic 38, 24–30.
10. Horák, D., Babic, M., Macková, H., and Benes, M. J. (2007) Preparation and properties of magnetic nano-and microsized particles for biological and environmental separations. Journal of Separation Science 30, 1751–1772.
11. Gulla, K. C., Gouda, M. D., Thakur, M. S., and Karanth, N. G. (2004) Enhancement of stability of immobilized glucose oxidase by modifica- tion of free thiols generated by reducing disulfide bonds and using additives. Biosensors and Bioelectronics 19, 621–625.
12. Wohlfahrt, G., Trivić, S., Zeremski, J., Peričin, D., and Leskovac, V. (2004) The chemical mechanism of action of glucose oxidase from Aspergillus niger. Molecular and Cellular Biochemistry 260, 69–83.
13. Rossi, L., Quach, A., and Rosenzweig, Z. (2004) Glucose oxidase-magnetite nanoparticle bioconjugate for glucose sensing. Analytical & Bioanalytical Chemistry 380, 606–613.
14. Kim, J., Jia, H., and Wang, P. (2006) Challenges in biocatalysis for enzyme-based biofuel cells. Biotechnology Advances 24, 296–308.
15. Mürbe, J., Rechtenbach, A., and Töpfer, J. (2008) Synthesis and physical characterization of magnetite nanoparticles for biomedical applications. Materials Chemistry and Physics 110, 426–433.
16. Tada, M., Hatanaka, S., Sanbonsugi, H., Matsushita, N., and Abe, M. (2003) Method for synthesizing ferrite nanoparticles ~ 30 nm in diameter on neutral pH condition for biomedical applications. Journal of Applied Physics 93, 7566–7568.