High-Performance Liquid Chromatography Analysis of N-Acyl Homoserine Lactone Hydrolysis by Paraoxonases

Methods in Molecular Biology

Volumn 692, Issue , 2011, Pages 291-298

  Teiber, John F ^a   Draganov, Dragomir I ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Acyl Homoserine Lactone; High performance liquid chromatography; Lactonase; Paraoxonases; Quorum sensing

Indexed keywords

ARYLDIALKYLPHOSPHATASE; ENZYME INHIBITOR; GAMMA BUTYROLACTONE DERIVATIVE;

ANIMAL; ARTICLE; DRUG ANTAGONISM; ENZYME ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; METHODOLOGY; MOUSE; ULTRAVIOLET SPECTROPHOTOMETRY;

ACYL-BUTYROLACTONES; ANIMALS; ARYLDIALKYLPHOSPHATASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME ASSAYS; ENZYME INHIBITORS; HYDROLYSIS; KINETICS; MICE; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 79952197625     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-971-0_21     Document Type: Chapter

References (4)

1. Draganov DI, Teiber JF, Speelman A, Osawa Y, Sunahara R, La Du BN. (2005) Human paraoxonases (PON1, PON2, and PON3) are lacto-nases with overlapping and distinct substrate specificities. J Lipid Res; 46(6):1239–1247.
2. Draganov D, Teiber J. (2008) PONs’ natural substrates-the key for their physiological roles. In: Mackness M, Aviram M, Parah G, editors. Protein and Cell Regulation, Volume 6: The Paraoxonases: Their Role in Disease Development and Xenobiotic Metabolism. Dordrecht: Springer: 297–306.
3. Stoltz DA, Ozer EA, Ng CJ et al. (2007) Paraoxonase-2 deficiency enhances Pseudomonas aeruginosa quorum sensing in murine tracheal epithelia. Am J Physiol Lung Cell Mol Physiol; 292(4):L852–L860.
4. Teiber JF, Horke S, Haines DC et al. (2008) Dominant role of paraoxonases in inactivation of the Pseudomonas aeruginosa quorum-sensing signal N-(3-oxododecanoyl)-L-homoserine lactone. Infect Immun; 76(6): 2512–2519.