Inhibition in radiation mediated cellular toxicity by minimizing free radical flux: One of the possible mechanisms of biological protection against lethal ionizing radiation by a sub-fraction of podophyllum hexandrum

Cellular and Molecular Biology

Volumn 56, Issue SUPPL.1, 2010, Pages

  Gupta, M L ^a   Gupta, V ^a   Shukla, S K ^a   Verma, S ^a   Sankhwar, S ^a   Dutta, A ^a   Suri, K A ^b  

^a INSTITUTE OF NUCLEAR MEDICINE AND ALLIED SCIENCES   (India)

^b CSIR INDIAN INSTITUTE OF INTEGRATIVE MEDICINE   (India)

Author keywords

Antioxidants; Free radical scavenging; Lipid peroxidation; Podophyllum hexandrum; Polyphenols; Radioprotection

Indexed keywords

1,1 DIPHENYL 2 PICRYLHYDRAZYL; FREE RADICAL; G 001M; G 100M; GLUTATHIONE; HYDROGEN PEROXIDE; MALONALDEHYDE; PHENOL; PLANT EXTRACT; PLASMID DNA; SUPEROXIDE; UNCLASSIFIED DRUG; 2,2-DIPHENYL-1-PICRYLHYDRAZYL; BIPHENYL DERIVATIVE; IRON CHELATING AGENT; PICRIC ACID; RADIOPROTECTIVE AGENT; SCAVENGER;

ANTIOXIDANT ACTIVITY; ARTICLE; CELL PROTECTION; CONTROLLED STUDY; CYTOTOXICITY; DOSE RESPONSE; DRUG EFFICACY; DRUG MECHANISM; FRACTIONATION; IONIZING RADIATION; LETHALITY; LEUKOCYTE COUNT; LIPID PEROXIDATION; MALE; MOUSE; NONHUMAN; PODOPHYLLUM HEXANDRUM; RADIATION PROTECTION; SPLEEN CELL; THYMOCYTE; WHOLE BODY RADIATION; ANIMAL; CHEMISTRY; DNA DAMAGE; DRUG EFFECT; METABOLISM; PODOPHYLLUM; RADIATION EXPOSURE; SPLEEN; THYROID GLAND;

MUS; SINOPODOPHYLLUM HEXANDRUM;

ANIMALS; BIPHENYL COMPOUNDS; DNA DAMAGE; FREE RADICAL SCAVENGERS; FREE RADICALS; GLUTATHIONE; IRON CHELATING AGENTS; LEUKOCYTE COUNT; LIPID PEROXIDATION; MALONDIALDEHYDE; MICE; PICRATES; PLANT EXTRACTS; PODOPHYLLUM; RADIATION, IONIZING; RADIATION-PROTECTIVE AGENTS; SPLEEN; THYROID GLAND;

EID: 79952196695     PISSN: 01455680     EISSN: 1165158X     Source Type: Journal    
DOI: 10.1170/153     Document Type: Article

References (32)

1. Benzie, I.F.F. and Strain, J.T., The Ferric Reducing Ability of Plasma (FRAP) as a Measure of "Antioxidant Power": The FRAP Assay. Anal. Biochem. 1996, 239: 70-76.
2. Beutler, E., Duron, O. and Kelly, B.M., Improved method for the determination of blood glutathione. J. Lab. Clin. Med. 1963, 61: 882-888.
3. Beutner, K.R., Podophyllotoxin in the treatment of genital warts. Curr. Probl. Dermatol. 1998, 24: 227-232.
4. Blokhina, O., Lainen, E.V. and Fagerstedt, K.V., Antioxidant, Oxidative damage, and oxygen deprivation stress: a review. Annals of Botany. 2003, 91: 179-194.
5. Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72: 248- 254.
6. Buege, J.A. and Aust, S., Microsomal lipid peroxidation. In: Methods in enzymology LII, Biomembranes. Academic Press, London, 1978.
7. Chawla, R., Arora, R., Kumar, R., Sharma, A.K., Prasad, J., Singh, S., Sagar, R.K., Chaudhary, P., Shukla, S. and Qazi, G.N., Antioxidant activity of fractionated extracts of high altitude Podophyllum hexandrum in radiation protection. Mol. Cell. Biochem. 2005, 273: 193-208.
8. Di Carlo, G., Mascolo, N., Izzo, A.A. and Capasso, F., Flavonoids: Old and new aspects of a class of natural therapeutic drugs. Life. Sci. 1999, 65: 337-353.
9. Goel, H.C., Prasad, J., Sharma, A. and Singh, B., Antitumor and radio protective action of Podophyllum hexandrum. Indian J. Exp. Biol. 1998, 36: 583-587.
10. Gupta, M.L., Agarwal, P.K., Kumar, I.P., Devi, M., Soni, N.L. and Tripathi, R.P., Modulation of gamma radiation-inflicted damage in swiss albino mice by an alcoholic fraction of P. hexandrum rhizome. J. Med. Food. 2008, 11: 486-92.
11. Gupta, M.L., Sankhwar, S., Verma, S., Devi, M., Samanta, N., Agarwala, P.K., Kumar, R. and Singh, P.K., Whole body protection to lethally irradiated mice by oral administration of semi purified fraction of Podophyllum hexandrum and post irradiation treatment of Picrorhiza kurroa. Tokai J. Exp. Clin. Med. 2008, 33: 6-12.
12. Gutpa, M.L., Tyagi, S., Flora, S.J.S.., Agarwala, P.K., Chaudhary, P., Puri, S.C., Devi, M., Sharma, A., Haksar, A., Qazi, G.N. and Tripathi, R.P., Protective efficacy of semi purified fraction of high altitude Podophylum hexandrum rhizomes in lethally irradiated swiss albino mice. Cell Mol. Biol. 2007, 53: 29-41.
13. Halliwell, B., Gutteridge, J.M.C. (1990) Role of free radicals and catalytic metal ions in human diseases: an overview. Meth. Enzymol 186: 1-84.
14. Kakkar, P., Das, B. and Vishwanathan, P.N., A modified spectrophotometric assay of superoxide dismutase. Indian J. Biophys. Biochem. 1984, 21: 130-133.
15. Lata, M., Prasad, J., Singh, S., Kumar, R., Singh, L., Chaudhary, P., Arora, R., Chawla, R., Tyagi, S., Soni, N.L., Sagar, R.K., Devi, M., Sharma, R.K., Puri, S.C. and Tripathi, R.P., Whole body protection against lethal ionizing radiation in mice by REC-2001: a semi-purified fraction of Podophyllum hexandrum. Phytomed. 2009, 16 : 47-55.
16. Miller, M.J., Sadowska-Krowicka, H., Chotinaruemol, S., Kakkis, J.L. and Clark, D.A., Amelioration of chronic ileitis by nitric oxide synthase inhibition. J. Pharmacol. Exp. Ther. 1993, 264: 11-16.
17. Mittal, A., Pathania, V., Agrawala, P.K., Prasad, J., Singh, S. and Goel, H.C., Influence of Podophyllum hexandrum on endogenous antioxidant defense system in mice: a possible role in radioprotection. J. Ethnopharmacol. 2001, 76: 253-262.
18. Molyneux, P., The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity Songklanakarin. J. Sci. Technol. 2004, 26: 212-219.
19. Riceevans, C.A., Miller, N.J. and Paganga, G., Antioxidant properties of phenolic compounds. Trends in Plant Science. 1997, 2: 152-159.
20. Rios, J. L., Giner, R. and M, Prieto., New findings on the bioactivity of lignans. Studies in Nat. Prod. 2002, 26: 183-292.
21. Robak, J. and Gryglewski, R.J., Flavonoids are scavengers of superoxide anion. Biochem. Pharmacol. 1988, 37: 837-841.
22. Ruch, R.J., Cheng, S.J. and Klaunig, J.E., Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 1989, 10: 1003-1008.
23. Sagar, R.K., Chawala, R., Arora, R., Singh, S., Krishna, B., Sharma, R.K., Puri, S.C., Singh, P., Kumar, R., Sharma, K.A., Singh, S., Prashad, J., Gupta, V., Ahmed, B., Singh, K.L., Khan, H.A., Gupta, M.L. and Quazi, G.N., Protection of the Hemopoietic System by Podophyllum hexandrum against Gamma Radiation-Induced Damage. Planta Med. 2006, 72: 114-120.
24. Sanghmitra, S., Gupta, M., Gupta, V., Verma, S., Suri, K.A., Devi, M., Sharma, P., Khan, E.A., Alam, M.S. and Tripathi, R.P., Podophyllum hexandrum- mediated survival protection and restoration of other cellular injuries in lethally irradiated mice. eCAM. 2009, 16: 47-55.
25. Sestili, P., Guidarelli, A., Dachà, M. and Cantoni, O., Quercetin Prevents DNA Single Strand Breakage and Cytotoxicity Caused By tert-Butylhydroperoxide: Free Radical Scavenging Versus Iron Chelating Mechanism. Free Rad. Biol. Med. 1998, 25: 196-200.
26. Shukla, S.K., Chaudhary, P., Kumar, I.P., Samanta, N., Afrin, F., Gupta, M.L., Sharma, U.K., Sinha, A.K., Sharma, Y.K. and Sharma, R.K., Protection from radiation-induced mitochondrial and genomic DNA damage by an extract of Hippophae rhamnoides. Environ. Mol. Mutagen. 2006, 47: 647-656.
27. Singh, J. and Shah, N.C., Podophyllum: A review. Curr. Res. Med. Arom. Plants. 1994, 16: 53-83.
28. Stahelin, H. and Von, Wartburg., A. From podophyllotoxin glucoside to etoposide. Prog. Drug. Res. 1989, 33 :169-267.
29. The antioxidant: Richard A passwater. Keat Publishing Incorporation, 1997.
30. Urquiaga, I. and Leighton, F., Plant Polyphenol Antioxidants and Oxidative Stress. Biol. Res. 2000, 33 N.2.
31. Yen, G.C., Duh, P.D. and Tsai, C.L., Relationship between antioxidant activity and maturity of peanut hulls. J. Agric. Food. Chem. 41, 1993: 67-70.
32. Ying-Quian, L., Yang, L. and Tian, M., Podophyllotoxin: Current Perspectives. Curr. Bioactive Compounds. 2007, 3: 37-66.