Fresh Frozen Tissue: RNA Extraction and Quality Control

Methods in Molecular Biology

Volumn 675, Issue , 2011, Pages 405-413

  Botling, Johan ^a   Micke, Patrick ^a  

^a UPPSALA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Bioanalyzer; Biobank; Fresh frozen tissue; RIN; RNA extraction; RNA quality

Indexed keywords

BIOBANK; GEL ELECTROPHORESIS; HUMAN TISSUE; QUALITY CONTROL; RNA EXTRACTION; TISSUE SECTION; ARTICLE; CAPILLARY ELECTROPHORESIS; FROZEN SECTION; GENETIC PROCEDURES; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY;

RNA;

ELECTROPHORESIS, CAPILLARY; FROZEN SECTIONS; GENETIC TECHNIQUES; HUMANS; QUALITY CONTROL; RNA;

MLCS; MLOWN;

EID: 79952117776     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-59745-423-0_25     Document Type: Chapter

References (14)

1. Morente, M.M., Mager, R., Alonso, S., Pezzella, F., Spatz, A., Knox, K., Kerr, D., Dinjens, W.N., Oosterhuis, J.W., Lam, K.H., Oomen, M.H., van Damme, B., van de Vijver, M., van Boven, H., Kerjaschki, D., Pammer, J., Lopez-Guerrero, J.A., Llombart Bosch, A., Carbone, A., Gloghini, A., Teodorovic, I., Isabelle, M., Passioukov, A., Lejeune, S., Therasse, P., van Veen, E.B., Ratcliffe, C., and Riegman, P.H. (2006) TuBaFrost 2: Standardising tissue collection and quality control procedures for a European virtual frozen tissue bank network. Eur J Cancer 42, 2684–2691.
2. Lin, D.W., Coleman, I.M., Hawley, S., Huang, C.Y., Dumpit, R., Gifford, D., Kezele, P., Hung, H., Knudsen, B.S., Kristal, A.R., and Nelson, P.S. (2006) Influence of surgical manipulation on prostate gene expression: implications for molecular correlates of treatment effects and disease prognosis. J Clin Oncol 24, 3763–3770.
3. Micke, P., Ohshima, M., Tahmasebpoor, S., Zhi-Ping, R., Östman, A., Ponten, F., and Botling J. (2006) Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens. Lab Invest 86, 202–211.
4. Breit, S., Nees, M., Schaefer, U., Pfoersich, M., Hagemeier, C., Muckenthaler, M., and Kulozik, A.E. (2004) Impact of pre-analytical handling on bone marrow mRNA gene expression. Br J Haematol 126, 231–243.
5. Ohashi, Y., Creek, K.E., Pirisi, L., Kalus, R., and Young, S.R. (2004) RNA degradation in human breast tissue after surgical removal: a time-course study. Exp Mol Pathol 77, 98–103.
6. Jewell, S.D., Srinivasan, M., McCart, L.M., Williams, N., Grizzle, W.H., LiVolsi, V., MacLennan, G., and Sedmak, D.D. (2002) Analysis of the molecular quality of human tissues: an experience from the Cooperative Human Tissue Network. Am J Clin Pathol 118, 733–741.
7. Huang, J., Qi, R., Quackenbush, J., Dauway, E., Lazaridis, E., and Yeatman, T. (2001) Effects of ischemia on gene expression. J Surg Res 99, 222–227.
8. Florell, S.R., Coffin, C.M., Holden, J.A., Zimmermann, J.W., Gerwels, J.W., Summers, B.K., Jones, D.A., and Leachman, S.A. (2001) Preservation of RNA for functional genomic studies: a multidisciplinary tumor bank protocol. Mod Pathol 14, 116–128.
9. Spruessel, A., Steimann, G., Jung, M., Lee, S.A., Carr, T., Fentz, A.K., Spangenberg, J., Zornig, C., Juhl, H.H., and David, K.A. (2004) Tissue ischemia time affects gene and protein expression patterns within minutes following surgical tumor excision. Biotechniques 36, 1030–1037.
10. Botling, J., Edlund, K., Segersten, U., Tahmasebpoor, S., Engström, M., Sundström, M., Malmström P.U., and Micke, P. (2009) Impact of thawing on RNA integrity and gene expression analysis in fresh frozen tissue. Diagn Mol Pathol 1, 44–52.
11. Micke, P., Ostman, A., Lundeberg, J., and Ponten, F. (2005) Laser-assisted cell microdissection using the PALM system. Methods Mol Biol 293, 151–166.
12. Mutter, G.L., Zahrieh, D., Liu, C., Neuberg, D., Finkelstein, D., Baker, H.E., and Warrington, J.A. (2006) Comparison of frozen and RNAlater solid tissue storage methods for use in RNA expression microarrays. BMC genomis 5, 88.
13. Lee, N.H., and Saeed, A.I. (2007) Microarrays: an overview. Methods Mol Biol 353, 265–300.
14. Imbeaud, S., Graudens, E., Boulanger, V., Barlet, X., Zaborski, P., Eveno, E., Mueller, O., Schroeder, A., and Auffray, C. (2005) Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. Nucleic Acids Res 33, e56.