Long-PCR Amplification of Human Genomic DNA

Methods in Molecular Biology

Volumn 688, Issue , 2011, Pages 67-74

  Keeney, Stephen ^a  

^a MANCHESTER UNIVERSITY NHS FOUNDATION TRUST   (United Kingdom)

Author keywords

DNA polymerase; F8 gene; Long PCR; Polymerase chain reaction; Primer design

Indexed keywords

BLOOD CLOTTING FACTOR 8; DNA; F8 PROTEIN, HUMAN;

AGAR GEL ELECTROPHORESIS; ARTICLE; FEMALE; GENETICS; HUMAN; HUMAN GENOME; INTRON; ISOLATION AND PURIFICATION; MALE; METHODOLOGY; POLYMERASE CHAIN REACTION;

DNA; ELECTROPHORESIS, AGAR GEL; FACTOR VIII; FEMALE; GENOME, HUMAN; HUMANS; INTRONS; MALE; POLYMERASE CHAIN REACTION;

EID: 79952117213     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-947-5_6     Document Type: Chapter

References (7)

1. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., and Erlich H. A. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487–91.
2. Barnes W. M. (1994) PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc Natl Acad Sci USA 91, 2216–20.
3. Cheng S., Fockler C., Barnes W. M., and Higuchi R. (1994) Effective amplification of long targets from cloned inserts and human genomic DNA. Proc Natl Acad Sci USA 91, 5695–99.
4. Liu Q., Nozari G., and Sommer S. S. (1998) Single-tube polymerase chain reaction for rapid diagnosis of the inversion hotspot of mutation in hemophilia A. Blood 92, 1458–9. Erratum in Blood (1999) 93, 2141.
5. Liu Q. and Sommer S. S. (1998) Subcycling-PCR for multiplex long distance amplification of regions with high and low GC content: application to the inversion hotspot in the FVIII gene. Biotechniques 25, 1022–28.
6. Lakich D., Kazazian H. H. Jr, Antonarakis S. E., and Gitschier J. (1993) Inversions disrupting the factor VIII gene are a common cause of severe haemophilia A. Nat Genet 5, 236–41.
7. Primer 3 URL: http://primer3.sourceforge. net/webif.php.