Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants

Methods in Molecular Biology

Volumn 702, Issue , 2011, Pages 151-161

  Decaunes, Pauline ^a   Estève, David ^a   Zakaroff Girard, Alexia ^a   Sengenès, Coralie ^a   Galitzky, Jean ^a   Bouloumié, Anne ^a  

^a UNIVERSITÉ PAUL SABATIER   (France)

Author keywords

Adipose tissue; Flow cytometry; Growth factors; Immunoselection; Inflammation; Obesity; Real time PCR

Indexed keywords

CD14 ANTIGEN; CD31 ANTIGEN; CD34 ANTIGEN; CYTOKINE; RNA;

ADIPOCYTE; ADIPOSE TISSUE; ARTICLE; CELL DIFFERENTIATION; CELL SEPARATION; CYTOLOGY; ENDOTHELIUM CELL; FLOW CYTOMETRY; HUMAN; ISOLATION AND PURIFICATION; LYMPHOCYTE; METABOLISM; METHODOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STROMA CELL;

ADIPOCYTES; ADIPOSE TISSUE; ANTIGENS, CD14; ANTIGENS, CD31; ANTIGENS, CD34; CELL DIFFERENTIATION; CELL SEPARATION; CYTOKINES; ENDOTHELIAL CELLS; FLOW CYTOMETRY; HUMANS; LYMPHOCYTES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; STROMAL CELLS;

MLCS; MLOWN;

EID: 79952117066     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61737-960-4_12     Document Type: Chapter

References (8)

1. Gimble J, Guilak F. (2003) Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy 5:362–369.
2. Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A. (2004) Improvement of postnatal neovascularization by human adipose tissue-derived stem cells. Circulation 110:349–355.
3. Planat-Benard V, Silvestre JS, Cousin B, Andre M, Nibbelink M, Tamarat R, Clergue M, Manneville C, Saillan-Barreau C, Duriez M, Tedgui A, Levy B, Penicaud L, Casteilla L. (2004) Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives. Circulation 109:656–663.
4. Rehman J, Traktuev D, Li J, Merfeld-Clauss S, Temm-Grove CJ, Bovenkerk JE, Pell CL, Johnstone BH, Considine RV, March KL. (2004) Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation 109:1292–1298.
5. Sengenes C, Lolmede K, Zakaroff-Girard A, Busse R, Bouloumie A. (2005) Preadipocytes in the human subcutaneous adipose tissue display distinct features from the adult mesenchymal and hematopoietic stem cells. J Cell Physiol 205:114–122.
6. Curat CA, Miranville A, Sengenes C, Diehl M, Tonus C, Busse R, Bouloumie A. (2004) From blood monocytes to adipose tissue-resident macrophages: induction of diapedesis by human mature adipocytes. Diabetes 53:1285–1292.
7. Duffaut C, Zakaroff-Girard A, Bourlier V, Decaunes P, Maumus M, Chiotasso P, Sengenes C, Lafontan M, Galitzky J, Bouloumie A. (2009) Interplay between human adipocytes and T lymphocytes in obesity. CCL20 as an adipochemokine and T lymphocytes as lipo- genic modulators. Arterioscler Thromb Vasc Biol 29:1608–14.
8. Curat CA, Wegner V, Sengenes C, Miranville A, Tonus C, Busse R, Bouloumie A. (2006) Macrophages in human visceral adipose tissue: increased accumulation in obesity and a source of resistin and visfatin. Diabetologia 49:744–747.