Use of Robotics in High-Throughput DNA Sequencing

Methods in Molecular Biology

Volumn 688, Issue , 2011, Pages 227-237

  Keeney, Stephen ^a  

^a MANCHESTER UNIVERSITY NHS FOUNDATION TRUST   (United Kingdom)

Author keywords

Cycle sequencing; DNA purification; Mutation analysis; Primer design; Robotics

Indexed keywords

ALCOHOL; DNA; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; EDETIC ACID;

ANALYSIS; ARTICLE; CHEMISTRY; DNA SEQUENCE; GENETICS; HIGH THROUGHPUT SCREENING; ISOLATION AND PURIFICATION; METHODOLOGY; MUTATION; POLYMERASE CHAIN REACTION; PRECIPITATION; ROBOTICS;

ANALYTIC SAMPLE PREPARATION METHODS; CHEMICAL PRECIPITATION; DNA; EDETIC ACID; ETHANOL; HIGH-THROUGHPUT SCREENING ASSAYS; INDICATORS AND REAGENTS; MUTATION; POLYMERASE CHAIN REACTION; ROBOTICS; SEQUENCE ANALYSIS, DNA;

EID: 79952116434     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-947-5_15     Document Type: Chapter

References (3)

1. Primer 3 (URL: http://primer3.sourceforge. net/webif.php)
2. National Genetics Reference Laboratory (Wessex) Application Note. Standardised Primer Optimisation and Design Specifi cation (URL: http://www.ngrl.org.uk/Wessex/ downloads_reports.htm)
3. Primers for F8 sequencing (URL: http://hadb.org.uk/WebPages/Database/Methods/pcr.html)