Purification of Rubisco Activase from Leaves or after Expression in Escherichia coli

Methods in Molecular Biology

Volumn 684, Issue , 2011, Pages 363-374

  Barta, Csengele ^a   Carmo Silva, A Elizabete ^a   Salvucci, Michael E ^a  

^a U S DEPARTMENT OF AGRICULTURE   (United States)

Author keywords

AAA+ protein; Arabidopsis thaliana; ATP; Calvin Cycle; CO2 fixation; Molecular chaperone; Rubisco activase

Indexed keywords

AMMONIUM SULFATE; RCA PROTEIN, PLANT; VEGETABLE PROTEIN;

ANIMAL; ARABIDOPSIS; ARTICLE; BIOSYNTHESIS; CELL PROLIFERATION; CHEMISTRY; CYTOLOGY; ENZYMOLOGY; ESCHERICHIA COLI; FRACTIONATION; GEL CHROMATOGRAPHY; GENE EXPRESSION; GENETICS; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METHODOLOGY; PLANT LEAF; PRECIPITATION;

AMMONIUM SULFATE; ANIMALS; ARABIDOPSIS; CELL PROLIFERATION; CHEMICAL FRACTIONATION; CHEMICAL PRECIPITATION; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; ESCHERICHIA COLI; GENE EXPRESSION; PLANT LEAVES; PLANT PROTEINS;

EID: 79952116203     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-925-3_28     Document Type: Chapter

References (20)

1. Salvucci, M. E., Portis, A. R. Jr., and Ogren, W. L. (1985) A soluble chloroplast protein catalyses ribulose-bisphosphate carboxylase/ oxygenase activation in vivo. Photosynth Res 7, 193–201.
2. Portis, A. R. Jr. (2003) Rubisco activase – Rubisco’s catalytic chaperone. Photosynth Res 75, 11–27.
3. Andrews, T. J. (1996) The bait in the Rubisco mousetrap. Nat Struct Biol 3, 3–7.
4. Portis, A. R. Jr., Li, C. S., Wang, D. F., and Salvucci, M. E. (2008) Regulation of Rubisco activase and its interaction with Rubisco. J Exp Bot 59, 1597–604.
5. Wang, Z. Y. and Portis, A. R. Jr. (1992) Dissociation of ribulose-1,5-bisphosphate bound to ribulose-1,5-bisphosphate carboxylase oxygenase and its enhancement by ribulose-1,5-bisphosphate carboxylase oxygenase activase mediated hydrolysis of ATP. Plant Physiol 99, 1348–53.
6. 2 response of ribu-lose-1,5-bisphosphate carboxylase/oxygenase activation in Arabidopsis leaves. Plant Physiol 80, 655–59.
7. Hammond, E. T., Andrews, T. J., and Woodrow, I. E. (1998) Regulation of ribu-lose-1,5-bisphosphate carboxylase/oxygenase by carbamylation and 2-carboxyarabinitol 1-phosphate in tobacco: Insights from studies of antisense plants containing reduced amounts of Rubisco activase. Plant Physiol 118, 1463–71.
8. 2 atmosphere. Plant Physiol 133, 1854–61.
9. Salvucci, M. E., Portis, A. R. Jr., Heber, U., and Ogren, W. L. (1987) Stimulation of thylakoid energization and ribulose-bisphosphate carboxylase/oxygenase activation in Arabidopsis leaves by methyl viologen. FEBS Lett 221, 215–20.
10. Salvucci, M. E. and Crafts-Brandner, S. J. (2004) Relationship between the heat tolerance of photosynthesis and the thermal stability of Rubisco activase in plants from contrasting thermal environments. Plant Physiol 134, 1460–70.
11. Salvucci, M. E., Werneke, J. M., Ogren, W. L., and Portis, A. R. Jr. (1987) Purification and species distribution of Rubisco activase. Plant Physiol 84, 930–6.
12. Streusand, V. J. and Portis, A. R. Jr. (1987) Rubisco activase mediates ATP-dependent activation of ribulose bisphosphate carboxylase. Plant Physiol 85, 152–4.
13. Robinson, S. P. and Portis, A. R. Jr. (1989) Adenosine-triphosphate hydrolysis by purified Rubisco activase. Arch Biochem Biophys 268, 93–9.
14. Robinson, S. P., Streusand, V. J., Chatfield, J. M., and Portis, A. R. Jr. (1988) Purification and assay of Rubisco activase from leaves. Plant Physiol 88, 1008–14.
15. Shen, J. B., Orozco, E. M., and Ogren, W. L. (1991) Expression of the two isoforms of spinach ribulose 1,5-bisphosphate carboxylase activase and essentiality of the conserved lysine in the consensus nucleotide-binding domain. J Biol Chem 266, 8963–8.
16. Salvucci, M. E., Rajagopalan, K., Sievert, G., Haley, B. E., and Watt, D. S. (1993) Photoaffinity labeling of ribulose-1.5-bispho-sphate carboxylase/oxygenase activase with ATP g-benzophenone. Identification of the ATP g-phosphate binding domain. J Biol Chem 268, 14239–44.
17. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal Biochem 72, 248–59.
18. Chua, N.-H. (1980) Electrophoretic analysis of chloroplast proteins. Methods Enzymol 69, 434–46.
19. van de Loo, F. J. and Salvucci, M. E. (1996) Activation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) involves Rubisco activase Trp16. Biochemistry 35, 8143–48.
20. 2+ and ATP or adenosine 5¢-[g-thio]-triphosphate (ATP-g-S) enhances intrinsic fluorescence and induces aggregation which increases the activity of spinach Rubisco activase. Biochim Biophys Acta 1202, 47–55.