Protein Targeting Across and into Chloroplast Membranes

Methods in Molecular Biology

Volumn 684, Issue , 2011, Pages 139-157

  Lo, Shari M ^a   Theg, Steven M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Chloroplast protein import; cpSec pathway; cpSRP pathway; cpTat pathway; Protein transport; Spontaneous insertion pathway

Indexed keywords

VEGETABLE PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CHLOROPLAST; CYTOLOGY; GENETIC TRANSCRIPTION; GENETICS; GROWTH, DEVELOPMENT AND AGING; INTRACELLULAR MEMBRANE; METABOLISM; PEA; PHOTOSYNTHESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SYNTHESIS; PROTEIN TRANSPORT; THYLAKOID;

ANIMALS; CHLOROPLASTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; INTRACELLULAR MEMBRANES; PEAS; PHOTOSYNTHESIS; PLANT PROTEINS; PROTEIN BIOSYNTHESIS; PROTEIN TRANSPORT; THYLAKOIDS; TRANSCRIPTION, GENETIC;

MLCS; MLOWN;

EID: 79952115702     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-925-3_13     Document Type: Chapter

References (20)

1. Aldridge, C., Cain, P., and Robinson, C. (2009) Protein transport in organelles: Protein transport into and across the thylakoid membrane. FEBS J 276, 1177–86.
2. Choquet, Y. and Vallon, O. (2000) Synthesis, assembly and degradation of thylakoid membrane proteins. Biochimie 82, 615–34.
3. Barber, J., Nield, J., Morris, E. P., Zheleva, D., and Hankamer, B. (1997) The structure, function, and dynamics of photosystem two. Physiol Plant 100, 817–27.
4. Jensen, P. E., Bassi, R., Boekema, E. J., Dekker, J. P., Jansson, S., Leister, D., Robinson, C., and Scheller, H. V. (2007) Structure, function and regulation of plant photosystem I. Biochim Biophys Acta 1767, 335–52.
5. Jarvis, P. (2008) Targeting of nucleus-encoded proteins to chloroplasts in plants. New Phytol 179, 257–85.
6. Hashimoto, A., Ettinger, W. F., Yamamoto, Y., and Theg, S. M. (1997) Assembly of newly imported oxygen-evolving complex subunits in isolated chloroplasts: Sites of assembly and mechanism of binding. Plant Cell 9, 441–52.
7. Schagger, H. (2006) Tricine-SDS-PAGE. Nat Protoc 1, 16–22.
8. Hynds, P. J., Robinson, D., and Robinson, C. (1998) The sec-independent twin-arginine translocation system can transport both tightly folded and malfolded proteins across the thylakoid membrane. J Biol Chem 273, 34868–74.
9. Cline, K. and McCaffery, M. (2007) Evidence for a dynamic and transient pathway through the TAT protein transport machinery. EMBO J 26, 3039–49.
10. Yuan, J. and Cline, K. (1994) Plastocyanin and the 33-kDa subunit of the oxygen-evolving complex are transported into thylakoids with similar requirements as predicted from pathway specificity. J Biol Chem 269, 18463–7.
11. Hulford, A., Hazell, L., Mould, R. M., and Robinson, C. (1994) Two distinct mechanisms for the translocation of proteins across the thylakoid membrane, one requiring the presence of a stromal protein factor and nucleotide triphosphates. J Biol Chem 269, 3251–6.
12. Cline, K. (1986) Import of proteins into chloroplasts: Membrane integration of a thylakoid precursor protein reconstituted in chloroplast lysates. J Biol Chem 261, 14804–10.
13. Yuan, J., Kight, A., Goforth, R. L., Moore, M., Peterson, E. C., Sakon, J., and Henry, R. (2002) ATP stimulates signal recognition particle (SRP)/FtsY-supported protein integration in chloroplasts. J Biol Chem 277, 32400–4.
14. Kim, S. U., Robinson, D., and Robinson, C. (1996) An Arabidopsis thaliana cDNA encoding PS II-X, a 4.1 kDa component of photosystem II: A bipartite presequence mediates SecA/DELTA-pH-independent targeting into thylakoids. FEBS Lett 390, 175–8.
15. Thompson, S. J., Kim, S. J., and Robinson, C. (1998) Sec-independent insertion of thylakoid membrane proteins – Analysis of insertion forces and identification of a loop intermediate involving the signal peptide. J Biol Chem 273, 18979–83.
16. Leheny, E. A. and Theg, S. M. (1994) Apparent inhibition of chloroplast protein import by cold temperatures is due to energetic considerations not membrane fluidity. Plant Cell 6, 427–37.
17. Reed, J. E., Cline, K., Stephens, L. C., Bacot, K. O., and Viitanen, P. V. (1990) Early events in the import/assembly pathway of an integral thylakoid protein. Eur J Biochem 194, 33–42.
18. Theg, S. M., Bauerle, C., Olsen, L. J., Selman, B. R., and Keegstra, K. (1989) Internal ATP is the only energy requirement for the translocation of precursor proteins across chloroplastic membranes. J Biol Chem 264, 6730–6.
19. Keegstra, K., Werner-Washburne, M., Cline, K., and Andrews, J. (1984) The chloroplast envelope: Is it homologous with the double membranes of mitochondria and gram-negative bacteria? J Cell Biochem 24, 55–68.
20. Laskey, R. A. and Mills, A. D. (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem 56, 335–41.