Automated DNA Sequencing

Methods in Molecular Biology

Volumn 688, Issue , 2011, Pages 173-185

  Wallis, Yvonne ^a   Morrell, Natalie ^a  

^a BIRMINGHAM CHILDREN S HOSPITAL NHS FOUNDATION TRUST   (United Kingdom)

Author keywords

Applied Biosystems 3730 genetic analyzer; BigDye sequencing chemistry; CleanSeq post sequencing clean up; Ethanol precipitation; ExoSAP PCR clean up; Fluorescent cycle sequencing; Sequencing troubleshooting

Indexed keywords

ALCOHOL; DNA;

ARTICLE; AUTOMATION; CAPILLARY ELECTROPHORESIS; CHEMISTRY; DNA SEQUENCE; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; POLYMERASE CHAIN REACTION; PRECIPITATION;

AUTOMATION; CHEMICAL PRECIPITATION; DNA; ELECTROPHORESIS, CAPILLARY; ETHANOL; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

EID: 79952115666     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-947-5_12     Document Type: Chapter

References (2)

1. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) DNA sequencing with chain terminating inhibitors. Proc. Natl Acad. Sci. USA 74, 5463–5467.
2. Shendure, J., and Ji, H. (2008) Next-generation DNA sequencing. Nat. Biotechnol. 26, 1135–1145.