Dissection of the bridging pattern of bovicin HJ50, a lantibiotic containing a characteristic disulfide bridge

Microbiological Research

Volumn 166, Issue 3, 2011, Pages 146-154

  Lin, Yuheng ^a,b   Teng, Kunling ^a   Huan, Liandong ^a   Zhong, Jin ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Bovicin HJ50; Disulfide bridge; Lantibiotics; Semi in vitro biosynthesis; Structural dissection

Indexed keywords

BOVICIN HJ50; DISULFIDE BRIDGE; LANTIBIOTICS; SEMI-IN; STRUCTURAL DISSECTION;

BIOSYNTHESIS; CHEMICAL ANALYSIS; CHEMICAL MODIFICATION; ESCHERICHIA COLI;

BIOCHEMISTRY;

BACTERIOCIN; DISULFIDE; SULFIDE;

ANTIMICROBIAL ACTIVITY; BACTERIUM; BIOACTIVITY; ENZYME ACTIVITY;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; METABOLISM; PROTEIN PROCESSING; SITE DIRECTED MUTAGENESIS; STREPTOCOCCUS BOVIS;

BACTERIOCINS; DISULFIDES; ESCHERICHIA COLI; GENE EXPRESSION; MUTAGENESIS, SITE-DIRECTED; PROTEIN PROCESSING, POST-TRANSLATIONAL; STREPTOCOCCUS BOVIS; SULFIDES;

ESCHERICHIA COLI; STREPTOCOCCUS BOVIS;

EID: 79951955351     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micres.2010.05.001     Document Type: Article

References (25)

1. Bulaj G. Formation of disulfide bonds in proteins and peptides. Biotechnol Adv 2005, 23(1):87-92.
2. Chatterjee C., Paul M., Xie L., van der Donk W.A. Biosynthesis and mode of action of lantibiotics. Chem Rev 2005, 105(2):633-684.
3. Chen P., Novak J., Kirk M., Barnes S., Qi F., Caufield P.W. Structure-activity study of the lantibiotic mutacin II from Streptococcus mutans T8 by a gene replacement strategy. Appl Environ Microbiol 1998, 64(7):2335-2340.
4. Chiu J., March P.E., Lee R., Tillett D. Site-directed, ligase-independent mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4h. Nucleic Acids Res 2004, 32(21):e174.
5. Cintas L.M., Rodriguez J.M., Fernandez M.F., Sletten K., Nes I.F., Hernandez P.E., et al. Isolation and characterization of pediocin L50, a new bacteriocin from Pediococcus acidilactici with a broad inhibitory spectrum. Appl Environ Microbiol 1995, 61(7):2643-2648.
6. Dorenbos R., Stein T., Kabel J., Bruand C., Bolhuis A., Bron S., et al. Thiol-disulfide oxidoreductases are essential for the production of the lantibiotic sublancin 168. J Biol Chem 2002, 277(19):16682-16688.
7. Furgerson Ihnken L.A., Chatterjee C., van der Donk W.A. In vitro reconstitution and substrate specificity of a lantibiotic protease. Biochemistry 2008, 47(28):7352-7363.
8. Holo H., Jeknic Z., Daeschel M., Stevanovic S., Nes I.F. Plantaricin W from Lactobacillus plantarum belongs to a new family of two-peptide lantibiotics. Microbiology 2001, 147(Pt 3):643-651.
9. Jung G. Lantibiotics - ribosomally synthesized biologically-active polypeptides containing sulfide bridges and alpha-beta-didehydroamino acids. Angew Chem Int Ed (in English) 1991, 30(9):1051-1068.
10. Kabuki T., Uenishi H., Seto Y., Yoshioka T., Nakajima H. A unique lantibiotic, thermophilin 1277, containing a disulfide bridge and two thioether bridges. J Appl Microbiol 2009, 106(3):853-862.
11. Karakas Sen A., Narbad A., Horn N., Dodd H.M., Parr A.J., Colquhoun I., et al. Post-translational modification of nisin. The involvement of NisB in the dehydration process. Eur J Biochem 1999, 261(2):524-532.
12. Krull R.E., Chen P., Novak J., Kirk M., Barnes S., Baker J., et al. Biochemical structural analysis of the lantibiotic mutacin II. J Biol Chem 2000, 275(21):15845-15850.
13. Kuipers O.P., Bierbaum G., Ottenwalder B., Dodd H.M., Horn N., Metzger J., et al. Protein engineering of lantibiotics. Anton Leeuw 1996, 69(2):161-169.
14. Liu G., Zhong J., Ni J., Chen M., Xiao H., Huan L. Characteristics of the bovicin HJ50 gene cluster in Streptococcus bovis HJ50. Microbiology 2009, 155(Pt 2):584-593.
15. Meyer C., Bierbaum G., Heidrich C., Reis M., Suling J., Iglesias-Wind M.I., et al. Nucleotide sequence of the lantibiotic Pep5 biosynthetic gene cluster and functional analysis of PepP and PepC. Evidence for a role of PepC in thioether formation. Eur J Biochem 1995, 232(2):478-489.
16. Nagao J., Harada Y., Shioya K., Aso Y., Zendo T., Nakayama J., et al. Lanthionine introduction into nukacin ISK-1 prepeptide by co-expression with modification enzyme NukM in Escherichia coli. Biochem Biophys Res Commun 2005, 336(2):507-513.
17. Paik S.H., Chakicherla A., Hansen J.N. Identification and characterization of the structural and transporter genes for, and the chemical and biological properties of, sublancin 168, a novel lantibiotic produced by Bacillus subtilis 168. J Biol Chem 1998, 273(36):23134-23142.
18. Sambrook J., Fritsch E.F., Maniatis T. Molecular cloning: a laboratory manual 1989, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 2nd ed.
19. Schagger H. Tricine-SDS-PAGE. Nat Protoc 2006, 1(1):16-22.
20. Szekat C., Jack R.W., Skutlarek D., Farber H., Bierbaum G. Construction of an expression system for site-directed mutagenesis of the lantibiotic mersacidin. Appl Environ Microbiol 2003, 69(7):3777-3783.
21. van den Hooven H.W., Lagerwerf F.M., Heerma W., Haverkamp J., Piard J.C., Hilbers C.W., et al. The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis: location of the thioether bridges. FEBS Lett 1996, 391(3):317-322.
22. van der Meer J.R., Polman J., Beerthuyzen M.M., Siezen R.J., Kuipers O.P., De Vos W.M. Characterization of the Lactococcus lactis nisin A operon genes nisP, encoding a subtilisin-like serine protease involved in precursor processing, and nisR, encoding a regulatory protein involved in nisin biosynthesis. J Bacteriol 1993, 175(9):2578-2588.
23. van Kraaij C., Breukink E., Rollema H.S., Bongers R.S., Kosters H.A., de Kruijff B., et al. Engineering a disulfide bond and free thiols in the lantibiotic nisin Z. Eur J Biochem 2000, 267(3):901-909.
24. Xiao H., Chen X., Chen M., Tang S., Zhao X., Huan L. Bovicin HJ50, a novel lantibiotic produced by Streptococcus bovis HJ50. Microbiology 2004, 150(Pt 1):103-108.
25. Xie L., Miller L.M., Chatterjee C., Averin O., Kelleher N.L., van der Donk W.A. Lacticin 481: in vitro reconstitution of lantibiotic synthetase activity. Science 2004, 303(5658):679-681.