Expression and purification of a functionally active class i fungal hydrophobin from the entomopathogenic fungus Beauveria bassiana in E. coli

Journal of Industrial Microbiology and Biotechnology

Volumn 38, Issue 2, 2011, Pages 327-335

  Kirkland, Brett H ^a   Keyhani, Nemat O ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

Beauveria bassiana; E. coli expression; Hydrophobin; Recombinant protein

Indexed keywords

HYDROPHOBIN; THIOFLAVINE;

ARTICLE; ATOMIC FORCE MICROSCOPY; BEAUVERIA BASSIANA; CELL INCLUSION; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; SURFACE PROPERTY; TRANSMISSION ELECTRON MICROSCOPY;

BEAUVERIA; ESCHERICHIA COLI; FUNGAL PROTEINS; MEMBRANE PROTEINS; MICROSCOPY, ATOMIC FORCE; MICROSCOPY, ELECTRON, TRANSMISSION; PROTEIN BINDING; RECOMBINANT PROTEINS;

CORDYCEPS BASSIANA; FUNGI;

EID: 79951671768     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-010-0777-7     Document Type: Article

References (48)

1. MHJ Akanbi E Post A Meter-Arkema R Rink GT Robillard XQ Wang HAB Wosten K Scholtmeijer 2010 Use of hydrophobins in formulation of water insoluble drugs for oral administration Colloids Surf B Biointerfaces 75 526 531 10.1016/j.colsurfb.2009.09.030
2. P Albuquerque CM Kyaw RR Saldanha MM Brigido MS Felipe I Silva-Pereira 2004 Pbhyd1 and Pbhyd2: two mycelium-specific hydrophobin genes from the dimorphic fungus Paracoccidioides brasiliensis Fungal Genet Biol 41 510 520 1:CAS:528:DC%2BD2cXis1Wgsbg%3D 10.1016/j.fgb.2004.01.001 15050540 (Pubitemid 38429774)
3. S Askolin T Nakari-Setala M Tenkanen 2001 Overproduction, purification, and characterization of the Trichoderma reesei hydrophobin HFBI Appl Microbiol Biotechnol 57 124 130 1:CAS:528:DC%2BD3MXotVSnu7c%3D 10.1007/s002530100728 11693908 (Pubitemid 32938361)
4. EM Cho L Liu W Farmerie NO Keyhani 2006 EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana I. Evidence for stage-specific gene expression in aerial conidia, in vitro blastospores and submerged conidia. Microbiology-Sgm 152 2843 2854 1:CAS:528:DC%2BD28XhtVart7bM
5. EM Cho BH Kirkland DJ Holder NO Keyhani 2007 Phage display cDNA cloning and expression analysis of hydrophobins from the entomopathogenic fungus Beauveria (Cordyceps) bassiana Microbiology-Sgm 153 3438 3447 1:CAS:528:DC%2BD2sXht1SmurvF 10.1099/mic.0.2007/008532-0 (Pubitemid 47629548)
6. Y Corvis A Walcarius R Rink NT Mrabet E Rogalska 2005 Preparing catalytic surfaces for sensing applications by immobilizing enzymes via hydrophobin layers Anal Chem 77 1622 1630 1:CAS:528:DC%2BD2MXhtlCgs7k%3D 10.1021/ac048897w 15762565 (Pubitemid 40396254)
7. ML de Vocht K Scholtmeijer EW van der Vegte, et al. 1998 Structural characterization of the hydrophobin SC3, as a monomer and after self-assembly at hydrophobic/hydrophilic interfaces Biophys J 74 2059 2068 10.1016/S0006- 3495(98)77912-3 9545064 (Pubitemid 28157940)
8. ML de Vocht I Reviakine HAB Wosten A Brisson JGH Wessels GT Robillard 2000 Structural and functional role of the disulfide bridges in the hydrophobin SC3 J Biol Chem 275 28428 28432 10.1074/jbc.M000691200 10829014
9. ML de Vocht I Reviakine WP Ulrich W Bergsma-Schutter HA Wosten H Vogel A Brisson JG Wessels GT Robillard 2002 Self-assembly of the hydrophobin SC3 proceeds via two structural intermediates Protein Sci 11 1199 1205 10.1110/ps.4540102 11967376 (Pubitemid 34441238)
10. GJ Grogan HL Holland 2000 The biocatalytic reactions of Beauveria spp J Mol Catal B Enzym 9 1 32 1:CAS:528:DC%2BD3cXhslalu78%3D 10.1016/S1381-1177(99) 00080-6 (Pubitemid 30136048)
11. CPR Hackenberger MM Chen B Imperiali 2006 Expression of N-terminal Cys-protein fragments using an intein refolding strategy Bioorg Med Chem 14 5043 5048 1:CAS:528:DC%2BD28XltFOmu74%3D 10.1016/j.bmc.2006.03.003 16554163 (Pubitemid 43767116)
12. J Hakanpaa A Paananen S Askolin T Nakari-Setala T Parkkinen M Penttila MB Linder J Rouvinen 2004 Atomic resolution structure of the HFBII hydrophobin, a self-assembling amphiphile J Biol Chem 279 534 539 10.1074/jbc.M309650200 14555650 (Pubitemid 38044855)
13. J Hakanpaa GR Szilvay H Kaljunen M Maksimainen M Linder J Rouvinen 2006 Two crystal structures of Trichoderma reesei hydrophobin HFBI - The structure of a protein amphiphile with and without detergent interaction Protein Sci 15 2129 2140 1:CAS:528:DC%2BD28Xpt1ejsbw%3D 10.1110/ps.062326706 16882996 (Pubitemid 44316015)
14. HJ Hektor K Scholtmeijer 2005 Hydrophobins: proteins with potential Curr Opin Biotechnol 16 434 439 1:CAS:528:DC%2BD2MXntVCqtb0%3D 10.1016/j.copbio.2005. 05.004 15950452 (Pubitemid 41111530)
15. DJ Holder NO Keyhani 2005 Adhesion of the entomopathogenic fungus Beauveria (Cordyceps) bassiana to substrata Appl Environ Microbiol 71 5260 5266 1:CAS:528:DC%2BD2MXhtVahtr7I 10.1128/AEM.71.9.5260-5266.2005 16151112 (Pubitemid 41338256)
16. DJ Holder BH Kirkland MW Lewis NO Keyhani 2007 Surface characteristics of the entomopathogenic fungus Beauveria (Cordyceps) bassiana Microbiology-Sgm 153 3448 3457 1:CAS:528:DC%2BD2sXht1SmurjM 10.1099/mic.0.2007/008524-0 (Pubitemid 47629549)
17. MA Jackson CA Dunlap ST Jaronski 2010 Ecological considerations in producing and formulating fungal entomopathogens for use in insect biocontrol Biocontrol 55 129 145 10.1007/s10526-009-9240-y
18. MI Janssen MBM van Leeuwen K Scholtmeijer TG van Kooten L Dijkhuizen HAB Wosten 2002 Coating with genetic engineered hydrophobin promotes growth of fibroblasts on a hydrophobic solid Biomaterials 23 4847 4854 1:CAS:528:DC%2BD38XntVWntbo%3D 10.1016/S0142-9612(02)00240-5 12361625 (Pubitemid 35286707)
19. P Kazmierczak DH Kim M Turina NK Van Alfen 2005 A hydrophobin of the chestnut blight fungus, Cryphonectria parasitica, is required for stromal pustule eruption Eukaryot Cell 4 931 936 1:CAS:528:DC%2BD2MXksFaltro%3D 10.1128/EC.4.5.931-936.2005 15879527 (Pubitemid 40697757)
20. AH Kwan RD Winefield M Sunde JM Matthews RG Haverkamp MD Templeton JP Mackay 2006 Structural basis for rodlet assembly in fungal hydrophobins Proc Natl Acad Sci U S A 103 3621 3626 1:CAS:528:DC%2BD28XivFWju78%3D 10.1073/pnas.0505704103 16537446 (Pubitemid 43376605)
21. AH Kwan I Macindoe PV Vukasin, et al. 2008 The Cys3-Cys4 loop of the hydrophobin EAS is not required for rodlet formation and surface activity J Mol Biol 382 708 720 1:CAS:528:DC%2BD1cXhtVKisLfE 10.1016/j.jmb.2008.07.034 18674544
22. MW Lewis IV Robalino NO Keyhani 2009 Uptake of the fluorescent probe FM4-64 by hyphae and haemolymph-derived in vivo hyphal bodies of the entomopathogenic fungus Beauveria bassiana Microbiology-Sgm 155 3110 3120 1:CAS:528:DC%2BD1MXht1WktrbE 10.1099/mic.0.029165-0
23. MB Linder GR Szilvay T Nakari-Setala ME Penttila 2005 Hydrophobins: the protein-amphiphiles of filamentous fungi FEMS Microbiol Rev 29 877 896 1:CAS:528:DC%2BD2MXhtFSisLjL 10.1016/j.femsre.2005.01.004 16219510 (Pubitemid 41416842)
24. MB Linder 2009 Hydrophobins: Proteins that self assemble at interfaces Curr Opin Colloid Interface Sci 14 356 363 1:CAS:528:DC%2BD1MXhtVegsbzM 10.1016/j.cocis.2009.04.001
25. LG Lugones JS Bosscher K Scholtmeyer OMH deVries JGH Wessels 1996 An abundant hydrophobin (ABH1) farms hydrophobic rodlet layers in Agaricus bisporus fruiting bodies Microbiology UK 142 1321 1329 1:CAS:528:DyaK28XjtFyisL0%3D 10.1099/13500872-142-5-1321 (Pubitemid 26154943)
26. S Paris JP Debeaupuis R Crameri M Carey F Charles MC Prevost C Schmitt B Philippe JP Latge 2003 Conidial hydrophobins of Aspergillus fumigatus Appl Environ Microbiol 69 1581 1588 1:CAS:528:DC%2BD3sXitlClsLw%3D 10.1128/AEM.69.3.1581-1588.2003 12620846 (Pubitemid 36314298)
27. MM Penas SA Asgeirsdottir I Lasa FA Culianez-Macia AG Pisabarro JG Wessels L Ramirez 1998 Identification, characterization, and In situ detection of a fruit-body-specific hydrophobin of Pleurotus ostreatus Appl Environ Microbiol 64 4028 4034 1:CAS:528:DyaK1cXms1enu7o%3D 9758836 (Pubitemid 28463009)
28. M Qin LK Wang XZ Feng YL Yang R Wang C Wang L Yu B Shao MQ Qiao 2007 Bioactive surface modification of mica and poly(dimethylsiloxane) with hydrophobins for protein immobilization Langmuir 23 4465 4471 1:CAS:528:DC%2BD2sXisVOmtbk%3D 10.1021/la062744h 17341100 (Pubitemid 46643176)
29. R Sabate SJ Saupe 2007 Thioflavin T fluorescence anisotropy: An alternative technique for the study of amyloid aggregation Biochem Biophys Res Commun 360 135 138 1:CAS:528:DC%2BD2sXntFamsbg%3D 10.1016/j.bbrc.2007.06.063 17586468 (Pubitemid 46990334)
30. K Scholtmeijer JG Wessels HA Wosten 2001 Fungal hydrophobins in medical and technical applications Appl Microbiol Biotechnol 56 1 8 1:CAS:528: DC%2BD3MXls1Shtrw%3D 10.1007/s002530100632 11499914 (Pubitemid 32661535)
31. K Scholtmeijer MI Janssen B Gerssen ML de Vocht BM van Leeuwen TG van Kooten HA Wosten JG Wessels 2002 Surface modifications created by using engineered hydrophobins Appl Environ Microbiol 68 1367 1373 1:CAS:528: DC%2BD38XitFSjsL0%3D 10.1128/AEM.68.3.1367-1373.2002 11872489 (Pubitemid 34205378)
32. M Sunde AHY Kwan MD Templeton RE Beever JP Mackay 2008 Structural analysis of hydrophobins Micron 39 773 784 1:CAS:528:DC%2BD1cXps1Wgt78%3D 10.1016/j.micron.2007.08.003 17875392
33. GR Szilvay A Paananen K Laurikainen E Vuorimaa H Lemmetyinen J Peltonen MB Linder 2007 Self-assembled hydrophobin protein films at the air-water interface: structural analysis and molecular engineering Biochemistry 46 2345 2354 1:CAS:528:DC%2BD2sXhsFOktrg%3D 10.1021/bi602358h 17297923 (Pubitemid 46370554)
34. NJ Talbot MJ Kershaw GE Wakley O De Vries J Wessels JE Hamer 1996 MPG1 encodes a fungal hydrophobin involved in surface interactions during infection-related development of Magnaporthe grisea Plant Cell 8 985 999 1:CAS:528:DyaK28XjvFSqsLw%3D 10.1105/tpc.8.6.985 12239409 (Pubitemid 26329826)
35. NJ Talbot 1999 Fungal biology Coming up for air and sporulation. Nature 398 295 296 1:CAS:528:DyaK1MXitlCntb4%3D
36. W van der Vegt HC van der Mei HA Wosten JG Wessels HJ Busscher 1996 A comparison of the surface activity of the fungal hydrophobin SC3p with those of other proteins Biophys Chem 57 253 260 10.1016/0301-4622(95)00059-7 8573679
37. MA van Wetter HA Wosten JH Sietsma JG Wessels 2000 Hydrophobin gene expression affects hyphal wall composition in Schizophyllum commune Fungal Genet Biol 31 99 104 10.1006/fgbi.2000.1231 11170739 (Pubitemid 32182238)
38. A Wanchoo MW Lewis NO Keyhani 2009 Lectin mapping reveals stage-specific display of surface carbohydrates in in vitro and haemolymph-derived cells of the entomopathogenic fungus Beauveria bassiana Microbiology-Sgm 155 3121 3133 1:CAS:528:DC%2BD1MXht1WktrbF 10.1099/mic.0.029157-0
39. J Wessels O De Vries SA Asgeirsdottir F Schuren 1991 Hydrophobin genes involved in formation of aerial hyphae and fruit bodies in Schizophyllum Plant Cell 3 793 799 1:CAS:528:DyaK3MXmsFWru7c%3D 10.1105/tpc.3.8.793 12324614 (Pubitemid 21913742)
40. JG Wessels 1997 Hydrophobins: proteins that change the nature of the fungal surface Adv Microb Physiol 38 1 45 1:CAS:528:DyaK1cXktlOltw%3D%3D 10.1016/S0065-2911(08)60154-X 8922117 (Pubitemid 26377552)
41. H Wosten O De Vries J Wessels 1993 Interfacial self-assembly of a fungal hydrophobin into a hydrophobic rodlet layer Plant Cell 5 1567 1574 1:CAS:528:DyaK2cXjtFOqt7k%3D 10.1105/tpc.5.11.1567 12271047 (Pubitemid 2021803)
42. HA Wosten MA van Wetter LG Lugones HC van der Mei HJ Busscher JG Wessels 1999 How a fungus escapes the water to grow into the air Curr Biol 9 85 88 1:CAS:528:DyaK1MXhtVKjtbo%3D 10.1016/S0960-9822(99)80019-0 10021365 (Pubitemid 29083532)
43. HA Wosten ML de Vocht 2000 Hydrophobins, the fungal coat unravelled Biochim Biophys Acta 1469 79 86 1:CAS:528:DC%2BD3cXms1Wjsbw%3D 10998570
44. HA Wosten 2001 Hydrophobins: multipurpose proteins Annu Rev Microbiol 55 625 646 1:CAS:528:DC%2BD3MXnslKjtLc%3D 10.1146/annurev.micro.55.1.625 11544369 (Pubitemid 32978118)
45. Wraight SP, Jackson MA, de Kock SL (2001) Production, stabilization, and formulation of fungal biocontrol agenets. In: Butt TM, Jackson C, Magan N (eds) Fungi as biocontrol agents: progress problems, potential. CAB International, Wallingford, UK, pp 253-287
46. MQ Xu H Paulus SR Chong 2000 Fusions to self-splicing inteins for protein purification Applications of Chimeric Genes and Hybrid Proteins, Pt A 326 376 418 1:CAS:528:DC%2BD3cXot1SmsLc%3D 10.1016/S0076-6879(00)26066-7
47. SH Ying MG Feng 2004 Relationship between thermotolerance and hydrophobin-like proteins in aerial conidia of Beauveria bassiana and Paecilomyces fumosoroseus as fungal biocontrol agents J Appl Microbiol 97 323 331 1:CAS:528:DC%2BD2cXnt1Klu7o%3D 10.1111/j.1365-2672.2004.02311.x 15239698 (Pubitemid 38998663)
48. SH Ying MG Feng 2007 Means to mediating accumulation of hydrophobin-like proteins in the wall of Beauveria bassiana conidia for improved tolerance to thermal stress J Gen Appl Microbiol 53 309 314 1:CAS:528:DC%2BD2sXhsVeisrnF 10.2323/jgam.53.309 18057822