Molecular basis for the inability of an oxygen atom donor ligand to replace the natural sulfur donor heme axial ligand in cytochrome P450 catalysis. Spectroscopic characterization of the Cys436Ser CYP2B4 mutant

Archives of Biochemistry and Biophysics

Volumn 507, Issue 1, 2011, Pages 119-125

  Perera, Roshan ^a,c   Sono, Masanori ^a   Voegtle, Heather L ^a   Dawson, John H ^a,b  

^a UNIVERSITY OF SOUTH CAROLINA   (United States)

^b UNIVERSITY OF SOUTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF TEXAS AT ARLINGTON   (United States)

Author keywords

CYP2B4; CYP2B4 heme axial mutant; CYP2B4(C436S) mutant; Electronic absorption spectroscopy; Magnetic circular dichroism (MCD); Oxyferrous complex

Indexed keywords

COORDINATION COMPOUND; CYSTEINE; CYTOCHROME P450; CYTOCHROME P450 2B4; FERRIC ION; IRON; LIGAND; OXYGEN; PROTON; SERINE; SULFUR; CARBON MONOXIDE; DITHIONITE; HEME; IRON COMPLEX; NITRIC OXIDE;

ACIDITY; ARTICLE; ATOM; ATOMIC ABSORPTION SPECTROMETRY; CATALYSIS; CHEMICAL BOND; CIRCULAR DICHROISM; CONTROLLED STUDY; DONOR; DRUG STRUCTURE; ELECTRON; HUMAN; LIGATION; MOLECULAR DYNAMICS; MUTANT; OXIDATION; PRIORITY JOURNAL; SPECTROSCOPY; ABSORPTION SPECTROSCOPY; MOLECULE; STRUCTURE ANALYSIS;

ANIMALS; ARYL HYDROCARBON HYDROXYLASES; CARBON MONOXIDE; CIRCULAR DICHROISM; CYSTEINE; HEME; IRON; LIGANDS; MODELS, MOLECULAR; NITROGEN OXIDES; OXYGEN; POINT MUTATION; SPECTROPHOTOMETRY; SULFUR;

EID: 79951566630     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2010.12.010     Document Type: Article

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