Detection and characterization of Salmonella in lairage, on pig carcasses and intestines in five slaughterhouses

International Journal of Food Microbiology

Volumn 145, Issue 1, 2011, Pages 279-286

  De Busser, E V ^a   Maes, D ^a   Houf, K ^a   Dewulf, J ^a   Imberechts, H ^b   Bertrand, S ^c   De Zutter, L ^a  

^a GHENT UNIVERSITY   (Belgium)

^b VETERINARY AND AGROCHEMICAL RESEARCH CENTRE   (Belgium)

^c SCIENTIFIC INSTITUTE OF PUBLIC HEALTH   (Belgium)

Author keywords

Carcass; Intestines; Lairage area; PFGE; Pig slaughterhouse; Salmonella

Indexed keywords

ANIMAL TISSUE; ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM DETECTION; BACTERIUM ISOLATION; CARCASS; CONTROLLED STUDY; COOLING; DUODENUM; ENVIRONMENTAL FACTOR; FOOD CONTAMINATION; GENOTYPE; ILEUM; MESENTERY LYMPH NODE; MOUTH CAVITY; NONHUMAN; ORAL BIOPSY; PULSED FIELD GEL ELECTROPHORESIS; RECTUM; SALMONELLA; SALMONELLA DERBY; SALMONELLA TYPHIMURIUM; SEROTYPE; SLAUGHTERHOUSE; SWINE;

ABATTOIRS; ANIMALS; BELGIUM; ELECTROPHORESIS, GEL, PULSED-FIELD; FOOD CONTAMINATION; FOOD HANDLING; GENOTYPE; INTESTINES; LYMPH NODES; MEAT; MOUTH; PREVALENCE; RECTUM; RESTRICTION MAPPING; SALMONELLA; SEROTYPING; SWINE;

SALMONELLA; SALMONELLA TYPHIMURIUM; SUIDAE;

EID: 79951557269     PISSN: 01681605     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2011.01.009     Document Type: Article

References (42)

1. Alban L., Stärk K.D.C. Where should the effort be put to reduce the Salmonella prevalence in the slaughtered swine carcass effectively?. Preventive Veterinary Medicine 2005, 68:63-79.
2. Bender J.B., Hedberg C.W., Boxrud D.J., Besser J.M., Wicklund J.H., Smith K.E., Osterholm M.T. Use of molecular subtyping in surveillance for Salmonella enterica serotype Typhimurium. The New England Journal of Medicine 2001, 344:189-195.
3. Berends B.R., Van Knapen F., Snijders J.M., Mossel D.A. Identification and quantification of risk factors regarding Salmonella spp. on pork carcasses. International Journal of Food Microbiology 1997, 36:199-206.
4. Borch E., Nesbakken T., Christensen H. Hazard identification in swine slaughter with respect to foodborne bacteria. International Journal of Food Microbiology 1996, 30:9-25.
5. Botteldoorn N., Heyndrickx M., Rijpens N., Grijspeerdt K., Herman L. Salmonella on pig carcasses: positive pigs and cross contamination in the slaughterhouse. Journal of Applied Microbiology 2003, 95:891-903.
6. Boudry C., Korsak N., Jacob B., Etienne G., Théwis A., Daube G. Ecologie de Salmonella dans le tube digestif du porc à l'abattage et etude de la contamination des carcasses. Les Annales de Médecine Vétérinaire 2002, 146:353-360.
7. Davies P.R., Hurd H.S., Funk J.A., Fedorka-Cray P.J., Jones F.T. The role of contaminated feed in the epidemiology and control of Salmonella enterica in pork production. Foodborne Pathogens and Disease 2004, 1:202-215.
8. De Busser E.V., Dewulf J., Nollet N., Houf K., Schwarzer K., De Sadeleer L., De Zutter L., Maes D. Effect of organic acids in drinking water during the last two weeks prior to slaughter on Salmonella shedding by slaughter pigs and contamination of carcasses. Zoonoses and Public Health 2009, 56:129-136.
9. De Sadeleer L., Dewulf J., De Zutter L., Van der Stede Y., Ribbens S., De Busser E., Quoilin S., Houf K., Delhalle L., Grijspeerdt K., Maes D. A qualitative risk assessment for human salmonellosis due to the consumption of fresh pork in Belgium. Vlaams Diergeneeskundig Tijdschrift 2008, 78:34-41.
10. Opinion of the scientific panel on biological hazards on the request from the commission related to "Risk assessment and mitigation options of Salmonella in pig production". The EFSA Journal 2006, 341:1-131. EFSA, European Food Safety Authority.
11. Report of the Task Force on Zoonoses Data Collection on the analysis of the baseline survey on the prevalence of Salmonella in slaughter pigs, part A. The EFSA Journal 2008, 135:1-111. EFSA, European Food Safety Authority.
12. Scientific opinion of the panel on biological hazards on a request from the European Commission on a quantitative microbiological risk assessment on Salmonella in meat: source attribution for human salmonellosis from meat. The EFSA Journal 2008, 625:1-32. EFSA, European Food Safety Authority.
13. Fedorka-Cray P.J., Gray J.T., Wray C. Salmonella infections in pigs. Salmonella in Domestic Animals 2000, 191-207. CAB International, Wallingford. C. Wray, A. Wray (Eds.).
14. Gebreyes W.A., Thakur S., Davies P.R., Funk J.A., Altier C. Trends in antimicrobial resistance, phage types and integrons among Salmonella serotypes from pigs 1997-2000. Journal of Antimicrobial Chemotherapy 2004, 53:997-1003.
15. Ghafir Y., Daube G. Comparison of swabbing and destructive methods for microbiological pig carcass sampling. Letters in Applied Microbiology 2008, 47:322-326.
16. Hendriksen R.S., Bangtrakulnonth A., Pulsrikarn C., Pornreongwong S., Hasman H., Wook Song S., Aarestrup F.M. Antimicrobial resistance and molecular epidemiology of Salmonella Rissen from animals, food products and patients in Thailand and Denmark. Foodborne Pathogens and Disease 2008, 5:605-619.
17. Hurd H.S., Gailey J.K., McKean J.D., Rostagno M.H. Rapid infection in market-weight swine following exposure to a Salmonella Typhimurium-contaminated environment. American Journal of Veterinary Research 2001, 62:1194-1197.
18. Isaacson R.E., Firkins L.D., Wiegel R.M., Zuckermann F.A., Di Pietro J.A. Effect of transportation and feed withdrawal on shedding of Salmonella Typhimurium among experimentally infected pigs. American Journal of Veterinary Research 1999, 60:1155-1158.
19. Korsak N., Daube G., Ghafir Y., Chahed A., Jolly S., Vindevogel H. An efficient sampling technique used to detect four foodborne pathogens on pork and beef carcasses in nine Belgian abattoirs. Journal of Food Protection 1998, 61:535-554.
20. Our food, our health-healthy diet and safe food in the Netherlands 2006, RIVM Rapport 270555009. C.F.V. Kreijl, A.G.A.C. Knaap, J.M.A.V. Raaij (Eds.).
21. Letellier A., Messier S., Pare J., Menard J., Quessy S. Distribution of Salmonella in swine herds in Quebec. Veterinary Microbiology 1999, 67:299-306.
22. Liebana E., Guns D., Garcia-Migura L., Woodward M.J., Clifton-Handley F.A., Davies R.H. Molecular typing of Salmonella serotypes prevalent in animals in England: assessment of methodology. Journal of Clinical Microbiology 2001, 39:3609-3616.
23. Lukinmaa S., Schildt R., Rinttila T., Siitonen A. Salmonella Enteritidis phage types 1 and 4: pheno- and genotypic epidemiology of recent outbreaks in Finland. Journal of Clinical Microbiology 1999, 37:2176-2182.
24. Lyytikäinen O., Koort J., Ward L., Schildt R., Ruutu P., Japisson E., Timonen M., Siitonen A. Molecular epidemiology of an outbreak caused by Salmonella enterica serovar Newport in Finland and the United Kingdom. Epidemiology and Infection 2000, 124:185-192.
25. Nollet N., Maes D., De Zutter L., Duchateau L., Houf K., Huysmans K., Imberechts H., Geers R., de Kruif A., Van Hoof J. Risk factors for the herd-level bacteriologic prevalence of Salmonella in Belgian slaughter pigs. Preventive Veterinary Medicine 2004, 65:63-75.
26. NRSS, National Reference Centre for Salmonella and Shigella, 2008. Annual report on Salmonella and Shigella in Belgium in 2008, Institute of Public Health. Online: http://www.iph.fgov.be/bacterio//iframes/rapports/2008.
27. Popoff M.Y., Le Minor L. Antigenic Formulas of the Salmonella Serovars 1992, WHO Collaborating Centre for Reference and Research on Salmonella, Institute Pasteur, Paris, France.
28. Rasschaert G., Houf K., Imberechts H., Grijspeerdt K., De Zutter L., Heyndrickx M. Comparison of five repetitive-sequence-based PCR typing methods for molecular discrimination of Salmonella enterica isolates. Journal of Clinical Microbiology 2005, 43:3615-3623.
29. Ribot E.M., Fair M.A., Gautom R., Cameron D.N., Hunter S.B., Swaminathan B., Barrett T.J. Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathogens and Disease 2006, 3:59-67.
30. Rossel R., Jouffe L., Beloeil P.A. Analyse des facteurs associés à la contamination des carcasses de porcs par Salmonella à l'aide des réseaux bayésiens. Journées de la Recherche Porcine 2009, 41:43-48.
31. Rostagno M.H., Hurd H.S., McKean J.D. Salmonella enterica prevalence and serotype distribution in swine at slaughter. Proceedings of the Seventh International Safepork Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork, Verona, Italy, May 9-11 2007, 153-155.
32. Rostagno M.H., Eicher S.D., Lay D.C. Does pre-slaughter stress affect pork safety risk?. Proceedings of the 21st IPVS Congress, Vancouver, Canada, July 18-21, 2010 2010, 176.
33. Seidler T., Alter T., Krüger M., Fehlhaber K. Transport stress-consequences for bacterial translocation, endogenous contamination and bactericidal activity of serum of slaughter pigs. Berliner und Münchener Tierärztliche Wochenschrift 2001, 114:375-377.
34. Swanenburg M., Berend B.R., Urlings H.A.P., Snijders J.M.A., van Knapen F. Epidemiological investigations into the sources of Salmonella contamination of pork. Berliner und Münchener Tierärztliche Wochenschrift 2001, 114:356-359.
35. Valdezate S., Echeita A., Diez R., Usera M.A. Evaluation of phenotypic and genotypic markers for characterisation of the emerging gastroenteritis pathogen Salmonella Hadar. European Journal of Clinical Microbiology and Infectious Diseases 2000, 19:275-281.
36. Valdezate S., Vidal A., Herrera-Leon S., Pozo J., Rubio P., Usera M.A., Carvajal A., Echeita M.A. Salmonella Derby clonal spread from pork. Emerging Infectious Diseases 2005, 11:694-698.
37. van der Gaag M.A., Vos F., Saatkamp H.W., van Boven M., van Beek P., Huirne R.B.M. A state-transmission simulation model for the spread of Salmonella in the pork supply chain. European Journal of Operational Research 2004, 156:782-798.
38. Van Loock F., Ducoffre G., Dumont J.M., Libotte-Chasseur M.L., Imberechts H., Gouffaux M., Houins-Roulet J., Lamsens G., De Schrijver K., Bin N., Moreau A., De Zutter L., Daube G. Analysis of foodborne disease in Belgium in 1997. Acta Clinica Belgica 2000, 55:300-306.
39. Vieira-Pinto M., Tenreiro R., Martines C. Unveiling contamination sources and dissemination routes of Salmonella sp. in pigs at a Portuguese slaughterhouse through macrorestriction profiling by pulsed-field gel electrophoresis. International Journal of Food Microbiology 2006, 110:77-84.
40. Wheeler J.G., Sethi D., Cowden J.M., Wall P.G., Rodrigues L.C., Tompkins D.S., Hudson M.J., Roderick P.J. Study of infectious intestinal disease in England: rates in the community, presenting to general practice, and reported to national surveillance. The Infectious Intestinal Disease Study Executive. British Medical Journal 1999, 318:1046-1050.
41. Wonderling L., Pearce R., Morgan Wallace F., Call J.E., Feder I., Tamplin M., Luchansky J.B. Use of pulsed-field gel electrophoresis to characterize the heterogeneity and clonality of Salmonella isolates obtained from the carcasses and feces of swine at slaughter. Applied and Environmental Microbiology 2003, 69:4177-4182.
42. Wood R.L., Pospischil A., Rose R. Distribution of persistent Salmonella Typhimurium infection in internal organs of swine. American Journal of Veterinary Research 1989, 50:1015-1021.