Evaluation of the sensitivity and specificity of primer pairs and the efficiency of RNA extraction procedures to improve noroviral detection from oysters by nested reverse transcription-polymerase chain reaction

Journal of Microbiology

Volumn 48, Issue 5, 2010, Pages 586-593

  Lee, Cheonghoon ^a   Cheong, Sooryun ^a   Lee, Hee Jung ^b   Kwon, Miye ^a   Kang, Ilnam ^a   Oh, Eun Gyoung ^b   Yu, Hong Sik ^b   Shin, Soon Bum ^b   Kim, Sang Jong ^a  

^a Seoul National University   (South Korea)

^b National Fisheries Research and Development Institute (NFRDI)   (South Korea)

Author keywords

nested RT PCR; norovirus; oyster; primer sensitivity and specificity; RNA extraction efficiency

Indexed keywords

DIAGNOSTIC AGENT; PRIMER DNA; RNA;

ANIMAL; ARTICLE; COMPARATIVE STUDY; EVALUATION; FECES; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; METHODOLOGY; NOROVIRUS; OYSTER; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIROLOGY;

ANIMALS; DNA PRIMERS; FECES; HUMANS; NOROVIRUS; OSTREIDAE; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; VIROLOGY;

NOROVIRUS; OSTREIDAE;

EID: 79951538115     PISSN: 12258873     EISSN: 12258873     Source Type: Journal    
DOI: 10.1007/s12275-010-0047-4     Document Type: Article

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