Evaluation of a rapid and completely automated real-time reverse transcriptase PCR assay for diagnosis of enteroviral meningitis

Journal of Clinical Microbiology

Volumn 49, Issue 2, 2011, Pages 528-533

  Nolte, Frederick S ^a   Rogers, Beverly B ^b   Tang, Yi Wei ^c   Oberste, M Steven ^d   Robinson, Christine C ^e,h   Kehl, K Sue ^f   Rand, Kenneth A ^g   Rotbart, Harley A ^e,h   Romero, Jose R ^i,k   Nyquist, Ann Christine ^e,h   Persing, David H ^j  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b CHILDREN S MEDICAL CENTER   (United States)

^c VANDERBILT UNIVERSITY MEDICAL CENTER   (United States)

^d CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^e CHILDREN S HOSPITAL COLORADO   (United States)

^f Children's Hospital of Wisconsin   (United States)

^g UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^h UNIVERSITY OF COLORADO   (United States)

ⁱ UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^j CEPHEID   (United States)

^k UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

more..

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS RNA;

ADULT; ARTICLE; AUTOMATION; CEREBROSPINAL FLUID ANALYSIS; CHILD; CLINICAL FEATURE; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; ENTEROVIRUS; ENTEROVIRUS INFECTION; FEMALE; HUMAN; MAJOR CLINICAL STUDY; MALE; MULTICENTER STUDY; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRUS CULTURE; VIRUS MENINGITIS;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; CEREBROSPINAL FLUID; CHILD; CHILD, PRESCHOOL; ENTEROVIRUS; ENTEROVIRUS INFECTIONS; FEMALE; HUMANS; INFANT; INFANT, NEWBORN; MALE; MENINGITIS, VIRAL; MIDDLE AGED; PREVALENCE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; TIME FACTORS; UNITED STATES; VIROLOGY; YOUNG ADULT;

ENTEROVIRUS;

EID: 79951494216     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01570-10     Document Type: Article

References (23)

1. Buck, G. E., M. Wiesemann, and L. Stewart. 2002. Comparison of mixed cell culture containing genetically engineered BGMK and CaCo-2 cells (Super E-Mix) with RT-PCR and conventional cell culture for the diagnosis of enterovirus meningitis. J. Clin. Virol. 25(Suppl.):S13.
2. Gartzonika, C., G. Vrioni, and S. Levidiotou. 2005. Evaluation of a commercially available reverse transcription-PCR enzyme immunoassay (Enterovirus Consensus kit) for the diagnosis of enterovirus central nervous system infections. Clin. Microbiol. Infect. 11:131-137.
3. Hamilton, M. S., M. A. Jackson, and D. Abel. 1999. Clinical utility of polymerase chain reaction testing for enteroviral meningitis. Pediatr. Infect. Dis. 18:533-537.
4. Kost, C. B., et al. 2007. Multicenter beta trial of the GeneXpert enterovirus assay. J. Clin. Microbiol. 45:1081-1086.
5. Marlowe, E. M., et al. 2008. Performance of the GeneXpert® enterovirus assay for detection of enteroviral RNA in cerebrospinal fluid. J. Clin. Virol. 43:110-113.
6. Marshall, G. S., M. A. Hauck, G. Buck, and G. P. Rabalais. 1997. Potential cost savings through rapid diagnosis of enteroviral meningitis. Pediatr. Infect. Dis. J. 16:1086-1087.
7. Modlin, J. F. 2007. Enterovirus déjàvu. N. Engl. J. Med. 356:1204-1205.
8. Nigrovic, L. E., R. Malley, D. Agarwall, and N. Kupperman for the Pediatric Emergency Medicine Collaborative Research Committee of the American Academy of Pediatrics. 2010. Low risk of bacterial meningitis in children with a positive enteroviral polymerase chain reaction test result. Clin. Infect. Dis. 55:1221-1222.
9. Nix, W. A., M. S. Oberste, and M. A. Pallansch. 2006. Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens. J. Clin. Microbiol. 44:2698-2704.
10. Ramers, C., G. Billman, M. Hartin, S. Ho, and M. H. Sawyer. 2000. Impact of a diagnostic cerebrospinal fluid enterovirus polymerase chain reaction test on patient management. JAMA 283:2680-2685.
11. Robinson, C. C., et al. 2002. Impact of rapid polymerase chain reaction results on management of pediatric patients with enteroviral meningitis. Pediatr. Infect. Dis. 21:283-286.
12. Romero, J. R. 1999. Reverse-transcription polymerase chain reaction detection of the enteroviruses. Arch. Pathol. Lab. Med. 123:1161-1169.
13. Rotbart, H. A. 1990. Diagnosis of enteroviral meningitis with the polymerase chain reaction. J. Pediatr. 117:85-89. (Pubitemid 20217501)
14. Rotbart, H. A., et al. 1994. Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay. J. Clin. Microbiol. 32:2590-2592.
15. Rotbart, H. A., et al. 1997. Diagnosis of enterovirus infection by polymerase chain reaction of multiple specimen types. Pediatr. Infect. Dis. 16:409-411.
16. Sawyer, M. H. 2001. Enterovirus infections: diagnosis and treatment. Curr. Opin. Pediatr. 13:65-69.
17. Sefers, S. E., A. K. Rayner, J. T. Kilby, D. H. Persing, and Y.-W. Tang. 2009. Prevalence and management of invalid GeneExpert enterovirus results obtained with cerebrospinal fluid samples: a 2-year study. J. Clin. Microbiol. 47:3008-3010.
18. Seme, K., et al. 2008. GeneXpert enterovirus assay: one-year experience in a routine laboratory setting and evaluation on three proficiency panels. J. Clin. Microbiol. 46:1510-1513.
19. Spicher, V. M., et al. 2000. Detection of enteroviruses in the cerebrospinal fluid by polymerase chain reaction: prospective study of impact on the management of hospitalized children. Clin. Pediatr. 39:203-208.
20. Stanway, G., P. Joki-Korpela, and T. Hyypia. 2000. Human parechoviurses: biology and clinical significance. Rev. Med. Virol. 10:57-69.
21. Strikas, R. A., L. J. Anderson, and R. A. Parker. 1986. Temporal and geographic patterns of isolates of nonpolio enteroviruses in the United States. J. Infect. Dis. 153:346-351.
22. Van Vliet, K. E., et al. 2001. Multicenter proficiency testing of nucleic acid amplification methods for the detection of enteroviruses. J. Clin. Microbiol. 39:3390-3392.
23. Wright, H., R. McAllister, and R. Ward. 1962. "Mixed" meningitis: report of a case with isolation of Haemophilus influenzae type b and echovirus type 9 from the cerebrospinal fluid. N. Engl. J. Med. 267:142-144.