Quantitative determination of phyllanthin in Phyllanthus amarus by high-performance thin layer chromatography

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas

Volumn 9, Issue 5, 2010, Pages 353-358

  Nayak, Preeti Sagar ^a   Upadhyay, Anubha ^a   Dwivedi, Sunil Kumar ^a   Rao, Sathrupa ^a  

^a Jawaharlal Nehru Krishi Vishwa Vidyalaya   (India)

Author keywords

HPTLC; Lignans; Phyllanthin; Phyllanthus amarus

Indexed keywords

ACETIC ACID ETHYL ESTER; ALCOHOL; HEXANE; LIGNAN; PHYLLANTHIN; PLANT MEDICINAL PRODUCT; SILICA GEL; SULFURIC ACID; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; BIOLOGICAL ACTIVITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; DRUG DETERMINATION; DRUG STRUCTURE; HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY; HIGH TEMPERATURE; PHYLLANTHUS AMARUS; PHYTOCHEMISTRY; PROCESS DEVELOPMENT; PROCESS MONITORING; PRODUCT RECOVERY; QUALITY CONTROL; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; SEPARATION TECHNIQUE; VALIDATION PROCESS;

PHYLLANTHUS AMARUS;

EID: 79851483009     PISSN: None     EISSN: 07177917     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Blumberg BS, Millman I, Venkateswaran PS and Thyagarajan SP, 1990. Hepatitis B virus and primary hepatocellular carcinoma: treatment of HBV carriers with Phyllanthus amarus. Vaccine 8: S86-S92.
2. Calixto JB, Santos AR, Cechinel Filho V, Yunes RA, 1998. A review of the plants of the genus Phyllanthus: their chemistry, pharmacology, and therapeutic potential. Med Res Rev. 18: 225-258.
3. Deb S. and Mandal SK, 1996. TLC-densitometric determination of phyllanthin and hypophyllanthin in Phyllanthus amarus (Bhumiamalaki) and in polyherbal formulations. Indian Drugs, 33: 415-416.
4. Gupta AP, Gupta MM, Kumar S. 1998. Estimation of Tropane alkaloids using high performance thin layer chromatography. J Ind Chem Soc 76: 277-278.
5. Gupta AP, Gupta MM, Kumar S. 1999a. High performance thin layer chromatography of asiaticoside in Centella asiatica. J Ind Chem Soc 76: 321-322.
6. Gupta AP, Gupta MM, Kumar S. 1999b. Simultaneous determination of curcuminoids in Curcuma samples using high performance thin layer chromatography. J. Liq. Chromatogr. Relat. Technol. 22: 1561-1569.
7. Gupta MM, Jain DC, Verma RK, Gupta AP. 1996. A rapid analytical method for the estimation of artemisinin in Artemisia annua. J. Med. Arom. Plant Sci 18: 5-6.
8. Gupta MM, Verma RK. 1996. Combined thin layer chromatography-densitometry method for the quantitative estimation of major alkaloids in poppy straw samples. Ind. J. Pharm. Sci 58: 161-163.
9. Jayaram S and Thyagarajan SP, 1996. Inhibition of HBsAg secretion from Alexander cell line by Phyllanthus amarus. Indian J. Pathol. Microbiol., 39: 211-215.
10. Kalasz H and Bathori, M. 1997. Present status and future perspectives of thin-layer chromatography. LC-GC Int. 10: 440-445.
11. Mukherjee PK. Quality Control of Herbal Drugs-An Approach to Evaluation of Botanicals. New Delhi: Business Horizons; 2002.
12. Mukherjee P.K. 2003. Plant products with hypochlesterolemic potentials. In: Taylor SL, ed. Advanced in Food and Nutrition Research. Vol. 47. New Delhi: Elsevier Science.
13. Munshi A, Mehrotra R, Ramesh R and Panda SK, 1993. Evaluation of anti-Hepadnavirus activity of Phyllanthus amarus and Phyllanthus maderaspatensis in duck hepatitis B virus carrier Pekin ducks. J. Med. Virol., 41: 275-281.
14. Murali B, Amit A, Anand MS, Dinesh TK and Samiulla DS, 2001. An improved HPLC method for estimation of phyllanthin and hypophyllanthin in Phyllanthus amarus. J. Natural Remedies 1: 55-59.
15. Oudhia P, Tripathi RS, 2002. Medicinal weed flora of brinjal (Solanum melongena L.) fields in Chhattisgarh (India). Crop Res. 20: 482-488.
16. Poole CF and Poole SK. 1994. Instrumental thin-layer chromatography. Anal. Chem. 66: 27A-37A.
17. Pothier J. 1996. Thin layer chromatography in plant science. In: Practical Thin Layer Chromatography-a Multidisciplinary Approach, Fried B, Sherma J (eds). CRC Press: Boca Raton, FL.
18. Rozylo JK. and Janicka M. 1991. Thermodynamic description of liquid-solid chromatography process in the optimization of separation conditions of organic compound mixture. J. Liq. Chromatogr. 14: 3197-3212.
19. Rozylo JK and Janicka M. 1996. Different planar techniques for prediction of solute retention in column liquid chromatography. J. Planar Chromatogr. 9: 418-424.
20. Sane RT, Chawla JL, Kuber VV. 1997. Studies on Phyllanthus amarus-Part-II. Indian Drugs, 34: 580-584.
21. Saxena S, Jain D.C, Gupta M.M, Sharma R.P, 2000. High performance thin layer chromatographic separation of hepatoprotective diterpenoids from Andrographis paniculata. Phytochem Anal 11: 34-36.
22. Shakila A, Rajeswari R. 2006. Effect ofGA3, certain chemicals and media on germination, growth and yield of Phyllanthus niruri. Plant Arch. 6: 121-125.
23. Sharma A, Singh RT and Handa SS 1993. Estimation of phyllanthin and hypophyllanthin by high performance liquid chromatography in Phyllanthus amarus Phytochem. Anal. 4: 226-229.
24. Sherma J. 1994. Planar chromatography. Anal. Chem. 66: 67R-83R.
25. Srivastava S, Gupta MM, Verma RK and Kumar S. 2000. Quantification of 1, 3 benzodioxanes derivatives from Piper mullesua. J Assoc Off Anal Chem 83: 1484-1488.
26. Thamlikitkul V, Wasuwat S and Kanchanapee P. 1991. Efficacy of Phyllanthus-Amarus for Eradication of Hepatitis B Virus in Chronic Carriers. J. Med. Assoc. Thailand, 74: 81-85.
27. Thyagarajan SP, Subramanian ST, Thirunalasundari, Venkateswaran PS and Blumberg BS, 1988. Effects of Phyllanthus amarus on chronic carriers of hepatitis B virus. Lancet 2: 764-766.
28. Venkateswaran PS, Millman I and Blumberg BS, 1987. Effect of an extract from Phyllanthus niruri on hepatitis B and woodchuck hepatitis viruses: In vivo and in viro studies. Proc. Natl. Acad. Sci. USA, 84: 274-278.
29. Weins, C. and Hauck, H.H., 1996. Advances and developments in thin layer chromatography. LC-GC Int. 9: 710-717.