Spontaneous proton transfer to a conserved intein residue determines on-pathway protein splicing

Journal of Molecular Biology

Volumn 406, Issue 3, 2011, Pages 430-442

  Pereira, Brian ^a,b,e   Shemella, Philip T ^c,f   Amitai, Gil ^a,g   Belfort, Georges ^b   Nayak, Saroj K ^a,c   Belfort, Marlene ^a,d  

^a WADSWORTH CENTER   (United States)

^b Rensselaer Polytechnic Institute   (United States)

^c Research Rensselaer Polytechnic Institute   (United States)

^d STATE UNIVERSITY OF NEW YORK   (United States)

^e Massachusetts Institute of Technology Cambridge   (United States)

^f IBM RESEARCH ZURICH   (Switzerland)

^g WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

FRET assay; intein mutagenesis; protein splicing; quantum mechanics; reaction mechanism

Indexed keywords

ASPARTIC ACID; EXTEIN; INTEIN;

ARTICLE; BIOTECHNOLOGICAL PROCEDURES; CARBOXY TERMINAL SEQUENCE; DEGRADATION KINETICS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; MOLECULAR BIOLOGY; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN PROCESSING; PROTEIN STRUCTURE; PROTON TRANSPORT; QUANTUM MECHANICS; REACTION ANALYSIS; SIMULATION; STATIC ELECTRICITY;

EID: 79551681995     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2010.12.024     Document Type: Article

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