Directed evolution of a protein container

Science

Volumn 331, Issue 6017, 2011, Pages 589-592

  Wörsdörfer, Bigna ^a   Woycechowsky, Kenneth J ^a,b   Hilvert, Donald ^a  

^a ETH ZURICH   (Switzerland)

^b UNIVERSITY OF UTAH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HUMAN IMMUNODEFICIENCY VIRUS PROTEINASE; PROTEIN;

CATALYSIS; COLIFORM BACTERIUM; COULOMB CRITERION; CYTOGENETICS; CYTOPLASM; ENZYME ACTIVITY; GENETIC ENGINEERING; HUMAN IMMUNODEFICIENCY VIRUS; MUTAGENICITY; MUTATION; PROTEIN; TOXIC SUBSTANCE;

AFFINITY CHROMATOGRAPHY; AQUIFEX AEOLICUS; ARTICLE; BIOSYNTHESIS; CATALYSIS; DIRECTED MOLECULAR EVOLUTION; DNA SEQUENCE; DNA SHUFFLING; ENCAPSULATION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HOST; MASS SPECTROMETRY; MUTAGENESIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN VARIANT; STATIC ELECTRICITY; VIRUS CAPSID; WESTERN BLOTTING;

AMINO ACID SEQUENCE; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; ESCHERICHIA COLI; HIV PROTEASE; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; POINT MUTATION; PROTEIN ENGINEERING; SELECTION, GENETIC; STATIC ELECTRICITY; TRANSFORMATION, BACTERIAL;

ESCHERICHIA COLI; MIRIDAE;

EID: 79551616431     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1199081     Document Type: Article

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35. We thank B. Roschitzki at the Functional Genomics Center Zürich for collection of mass spectrometic data and assistance with data analysis; T. Ishikawa and M. Lucas at the Electron Microscopy Center of the ETH Zürich (EMEZ) for collecting the EM images and assistance with EM data analysis; H.-M. Fischer, F. Narberhaus, M. Neuenschwander, and F. Seebeck for contributive discussions; and P. Kast for careful reading of the manuscript. This work was supported in part by the Schweizerischer Nationalfond and the ETH Zürich. The supercharged GFP described in fig. S8 was obtained under a materials transfer agreement (MTA) with D. R. Liu at Harvard. An MTA would be required for sharing of materials used in the reported research.