Novel multiplex real-time PCR system using the SNP technology for the simultaneous diagnosis of Chlamydia trachomatis, Ureaplasma parvum and Ureaplasma urealyticum and genetic typing of serovars of C. trachomatis and U. parvum in NGU

Molecular and Cellular Probes

Volumn 25, Issue 1, 2011, Pages 55-59

  Tang, Jingfeng ^a   Zhou, Li ^a   Liu, Xiaoying ^a   Zhang, Changming ^a   Zhao, Youyun ^a   Wang, Yefu ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

Genetic typing of serovars; Multiplex real time PCR; NGU; Rapid diagnosis; SNP

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; CHLAMYDIA TRACHOMATIS; CHLAMYDIASIS; DIAGNOSTIC VALUE; FEMALE; HUMAN; MAJOR CLINICAL STUDY; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SEROTYPE; SINGLE NUCLEOTIDE POLYMORPHISM; UREAPLASMA; UREAPLASMA INFECTION; UREAPLASMA PARVUM; UREAPLASMA UREALYTICUM;

CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; DNA PROBES; FEMALE; GENOTYPE; HUMANS; MALE; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; SEROTYPING; UREAPLASMA; UREAPLASMA INFECTIONS; UREAPLASMA UREALYTICUM; URETHRITIS;

CHLAMYDIA TRACHOMATIS; UREAPLASMA PARVUM; UREAPLASMA UREALYTICUM;

EID: 79551479823     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2010.12.001     Document Type: Article

References (16)

1. Xia Q., Xu S., Wang D., Wen Y., Qin X., Qian S., et al. Development of a novel quantitative real-time assay using duplex scorpion primer for detection of Chlamydia trachomatis. Exp Mol Pathol 2007, 83:119-124.
2. Robertson J., Stemke G., Davis J., Harasawa R., Thirkell D., Kong F., et al. Proposal of Ureaplasma parvum sp. nov. and emended description of Ureaplasma urealyticum (Shepard et al. 1974) Robertson et al. 2001. Int J Syst Evolut Microbiol 2002, 52:587-597.
3. Gdoura R., Kchaou W., Ammar-Keskes L., Chakroun N., Sellemi A., Znazen A., et al. Assessment of Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis, and Mycoplasma genitalium in semen and first void urine specimens of asymptomatic male partners of infertile couples. J Androl 2008, 29:198-206.
4. Tang J., Xu Z., Zhou L., Qin H., Wang Y., Wang H. Rapid and simultaneous detection of Ureaplasma parvum and Chlamydia trachomatis antibodies based on visual protein microarray using gold nanoparticles and silver enhancement. Diagn Microbiol Infect Dis 2010, 67:122-128.
5. Cao X., Wang Y., Hu X., Qing H., Wang H. Real-time TaqMan polymerase chain reaction assays for quantitative detection and differentiation of Ureaplasma urealyticum and Ureaplasma parvum. Diagn Microbiol Infect Dis 2007, 57:373-378.
6. Lijavetzky D., Cabezas J., Ibanez A., Rodriguez V., Martinez-Zapater J. High throughput SNP discovery and genotyping in grapevine (Vitis vinifera L.) by combining a re-sequencing approach and SNPlex technology. BMC Genom 2007, 8:424.
7. Kohler J., Cutler D. Simultaneous discovery and testing of deletions for disease association in SNP genotyping studies. Am J Hum Genet 2007, 81:684-699.
8. Umemoto T., Hrdlicka P., Babu B., Wengel J. Sensitive SNP dual-probe assays based on pyrene-functionalized 2'-amino-LNA: lessons to be learned. Chembiochem 2007, 8:2240-2248.
9. Housni H., Vandesompele J., Vannuffel P., Gulbis B., Parma J., Cochaux P. Rapid and easy prenatal diagnosis of sickle cell anemia using double-dye LNA probe technology. Br J Haematol 2007, 136:509-510.
10. Montone K., Feldman M. In situ detection of aspergillus 18s ribosomal RNA Sequences using a terminally biotinylated locked nucleic acid (LNA) probe. Diagn Mol Pathol 2009, 18:239-242.
11. Chousalkar K., Cheetham B., Roberts J. LNA probe-based real-time RT-PCR for the detection of infectious bronchitis virus from the oviduct of unvaccinated and vaccinated laying hens. J Virol Methods 2009, 155:67-71.
12. Cao X., Jiang Z., Wang Y., Gong R., Zhang C. Two multiplex real-time TaqMan polymerase chain reaction systems for simultaneous detecting and serotyping of Ureaplasma parvum. Diagn Microbiol Infect Dis 2007, 59:109-111.
13. Yi J., Yoon B., Kim E. Detection and biovar discrimination of Ureaplasma urealyticum by real-time PCR. Mol Cell Probes 2005, 19:255-260.
14. Zheng H., Jiang L., Fang D., Xue Y., Wu Y., Huang J., et al. Application of an oligonucleotide array assay for rapid detecting and genotyping of Chlamydia trachomatis from urogenital specimens. Diagn Microbiol Infect Dis 2007, 57:1-6.
15. Millman K., Black C., Stamm W., Jones R., Hook E., Martin D., et al. Population-based genetic epidemiologic analysis of Chlamydia trachomatis serotypes and lack of association between ompA polymorphisms and clinical phenotypes. Microbes Infect 2006, 8:604-611.
16. Xiao L., Glass J., Paralanov V., Yooseph S., Cassell G., Duffy L., et al. Detection and characterization of human Ureaplasma species and serovars by real-time PCR. J Clin Microbiol 2010, 48:2715-2723.