Reduction of immunogenicity of anthrax vaccines subjected to thermal stress, as measured by a toxin neutralization assay

Clinical and Vaccine Immunology

Volumn 18, Issue 2, 2011, Pages 349-351

  Castelán Vega, Juan ^a,b   Corvette, Laura ^a   Sirota, Lev ^a   Arciniega, Juan ^a  

^a CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

^b INSTITUTO POLITÉCNICO NACIONAL   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRAX VACCINE;

ANTIBODY RESPONSE; ARTICLE; CELL VIABILITY; CELLULAR IMMUNITY; CONTROLLED STUDY; IMMUNIZATION; IMMUNOASSAY; IMMUNOGENICITY; IN VITRO STUDY; LETHALITY; MOUSE; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE STRESS; TOXIN NEUTRALIZATION ASSAY;

ANIMALS; ANTHRAX VACCINES; ANTIBODIES, BACTERIAL; ANTITOXINS; DRUG STABILITY; HOT TEMPERATURE; MICE; NEUTRALIZATION TESTS;

EID: 79551471831     PISSN: 15566811     EISSN: 1556679X     Source Type: Journal    
DOI: 10.1128/CVI.00267-10     Document Type: Article

References (10)

1. Berthold, I., M. Pombo, L. Wagner, and J. L. Arciniega. 2005. Immunogenicity in mice of anthrax recombinant protective antigen in the presence of aluminum adjuvants. Vaccine 23:1993-1999.
2. Hansen, M. B., S. E. Nielsen, and K. Berg. 1989. Re-examination and further development of a precise and rapid dye method for measuring cell growth/ cell kill. J. Immunol. Methods 119:203-210.
3. Hering, D., et al. 2004. Validation of the anthrax lethal toxin neutralization assay. Biologicals 32:17-27.
4. Jiang, G., et al. 2006. Anthrax vaccine powder formulations for nasal mucosal delivery. J. Pharm. Sci. 95:80-96.
5. Klein, J., et al. 1999. Validation of assays for use with combination vaccines. Biologicals 27:35-41.
6. Li, H., et al. 2008. Standardized, mathematical model-based and validated in vitro analysis of anthrax lethal toxin neutralization. J. Immunol. Methods 333:89-106.
7. Radha, C., P. Salotra, R. Bhat, and R. Bhatnagar. 1996. Thermostabilization of protective antigen - the binding component of anthrax lethal toxin. J. Biotechnol. 50:235-242.
8. Reuveny, S., et al. 2001. Search for correlates of protective immunity conferred by anthrax vaccine. Infect. Immun. 69:2888-2893.
9. Singh, S., et al. 2002. Gln277 and Phe554 residues are involved in thermal inactivation of protective antigen of Bacillus anthracis. Biochem. Biophys. Res. Commun. 296:1058-1062.
10. Singh, S., et al. 2004. Thermal inactivation of protective antigen of Bacillus anthracis and its prevention by polyol osmolytes. Biochem. Biophys. Res. Commun. 322:1029-1037.