Isolating pure populations of monocytes from the blood of pregnant women: Comparison of flotation in iodixanol with elutriation

Journal of Immunological Methods

Volumn 293, Issue 1-2, 2004, Pages 215-218

  Nutt, Jeffrey C ^a,b   Willis, Catherine C ^a   Morris, Jonathan M ^a   Gallery, Eileen D M ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

^b ROYAL NORTH SHORE HOSPITAL   (Australia)

Author keywords

Centrifugation; Density gradient; Monocytes; Pregnancy

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; INTERLEUKIN 6; IODIXANOL; MONOCYTE CHEMOTACTIC PROTEIN 1;

ARTICLE; CELL ACTIVATION; CELL ISOLATION; CONTROLLED STUDY; CYTOKINE PRODUCTION; DENSITY GRADIENT CENTRIFUGATION; FEMALE; FLOTATION; HUMAN; INTERMETHOD COMPARISON; ISOLATION PROCEDURE; MATERNAL BLOOD; MONOCYTE; PREGNANCY; PRIORITY JOURNAL;

ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, MYELOMONOCYTIC; CELL SEPARATION; CONTRAST MEDIA; FEMALE; HUMANS; MONOCYTES; PREGNANCY; TRIIODOBENZOIC ACIDS;

BACTERIA (MICROORGANISMS);

EID: 7944231392     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2004.05.011     Document Type: Article

References (12)

1. L.N. Berge, M. Ostensen, and A. Revhaug Phagocytic cell activity in pre-eclampsia Acta Obstetricia et Gynecologica Scandinavica 67 1988 499
2. V.A. Danis, M. Millington, V.J. Hyland, and D. Grennan Cytokine production by normal human monocytes: inter-subject variation and relationship to an IL-1 receptor antagonist (IL-1 Ra) gene polymorphism Clinical and Experimental Immunology 99 1995 303
3. D. Davis, R. Kaufman, and E.J. Moticka Nonspecific immunity in pregnancy: monocyte surface Fcgamma receptor expression and function Journal of Reproductive Immunology 40 1998 119
4. E. Grage-Griebenow, H.-D. Flad, and M. Ernst Heterogeneity of human peripheral blood monocyte subsets Journal of Leukocyte Biology 69 2001 11
5. G.M. Graziani-Bowering, J.M. Graham, and L.G. Filion A quick, easy and inexpensive method for the isolation of human peripheral blood monocytes Journal of Immunological Methods 207 1997 157
6. T. Hawkins, M.P. Jones, and E.D.M. Gallery Secretion of prostanoids by platelets and monocytes in normal and hypertensive pregnancies American Journal of Obstetrics and Gynecology 168 1993 661
7. E. Koumandakis, I. Koumandaki, E. Kaklamani, L. Sparos, D. Avantinos, and D. Trichopoulos Enhanced phagocytosis of mononuclear phagocytes in pregnancy British Journal of Obstetrics and Gynaecology 93 1986 1150
8. P. Luppi, C. Haluszczak, D. Betters, C.A.H. Richard, and M. Trucco Monocytes are progressively activated in the circulation of pregnant women Journal of Leukocyte Biology 72 2002 874
9. N. Naccasha, M.-T. Gervasi, T. Chaiworapongsa, S. Berman, B.H. Yoon, E. Maymon, and R. Romero Phenotypic and metabolic characteristics of monocytes and granulocytes in normal pregnancy and maternal infection American Journal of Obstetrics and Gynecology 185 2001 1118
10. G.P. Sacks, K. Studena, I.L. Sargent, and C.W.G. Redman Normal pregnancy and pre-eclampsia both produce inflammatory changes in peripheral blood leukocytes akin to those of sepsis American Journal of Obstetrics and Gynecology 179 1998 80
11. P. von Dadelszen, T. Wilkins, and C.W. Redman Maternal peripheral blood leukocytes in normal and pre-eclamptic pregnancies British Journal of Obstetrics and Gynaecology 106 1999 576
12. C. Willis, J. Morris, V. Danis, and E. Gallery Cytokine production by peripheral blood monocytes during the normal human ovulatory menstrual cycle Human Reproduction 18 2003 1173