A fluorescent dual labeling technique for the quantitative measurement of reduced and oxidized protein thiols in tissue samples

Free Radical Biology and Medicine

Volumn 50, Issue 4, 2011, Pages 510-517

  Armstrong, Alex E ^a   Zerbes, Ralf ^a   Fournier, Paul A ^a   Arthur, Peter G ^a  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

Electrophoresis; Ellman's assay; Fluorescence; Free radicals; mdx; Muscle; Oxidative stress; Protein disulfide; Protein redox; Protein sulfhydryl; Protein thiol; Reactive oxygen species

Indexed keywords

MALEIMIDE DERIVATIVE; N ETHYLMALEIMIDE; PROTEIN; PROTEIN THIOL; TRIS(2 CARBOXYETHYL)PHOSPHINE; UNCLASSIFIED DRUG;

ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; FLUOROMETRY; MALE; MOUSE; MUSCLE TISSUE; MUSCULAR DYSTROPHY; NONHUMAN; OXIDATION REDUCTION STATE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; SKELETAL MUSCLE; SOLUBILIZATION;

ANIMALS; BORON COMPOUNDS; FLUORESCENT DYES; MALE; MALEIMIDES; MICE; MICE, INBRED C57BL; MICE, INBRED MDX; MUSCLE, SKELETAL; PROTEINS; STAINING AND LABELING; SULFHYDRYL COMPOUNDS; XANTHENES;

MUS;

EID: 79251609052     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.freeradbiomed.2010.11.018     Document Type: Article

References (38)

1. P. Eaton Protein thiol oxidation in health and disease: techniques for measuring disulfides and related modifications in complex protein mixtures Free Radic. Biol. Med. 40 2006 1889 1899
2. P. Di Simplicio Thiolation and nitrosation of cysteines in biological fluids and cells Amino Acids 25 2003 323 339
3. P. Klatt, and S. Lamas Regulation of protein function by S-glutathiolation in response to oxidative and nitrosative stress Eur. J. Biochem. 267 2000 4928 4944
4. M.M. Gallogly Mechanisms of reversible protein glutathionylation in redox signaling and oxidative stress Curr. Opin. Pharmacol. 7 2007 381 391
5. C.C. Winterbourn, and M.B. Hampton Thiol chemistry and specificity in redox signaling Free Radic. Biol. Med. 45 2008 549 561
6. L.F. Ferreira Muscle-derived ROS and thiol regulation in muscle fatigue J. Appl. Physiol. 104 2008 853 860
7. M.B. Reid Free radicals and muscle fatigue: of ROS, canaries, and the IOC Free Radic. Biol. Med. 44 2008 169 179
8. K.Q. Do Redox dysregulation, neurodevelopment, and schizophrenia Curr. Opin. Neurobiol. 19 2009 220 230
9. L.-P. Yap The energy-redox axis in aging and age-related neurodegeneration Adv. Drug Deliv. Rev. 61 2009 1283 1298
10. Qureshi G.A. Role of selenium, iron, copper, and zinc in parkinsonism Oxidative Stress and Neurodegenerative Disorders 2007 Elsevier Science Amsterdam 719 735
11. E. Dursun Protein oxidation in type 2 diabetic patients on hemodialysis J. Diab. Complications 19 2005 142 146
12. W. Dröge Aging-related changes in the thiol/disulfide redox state: implications for the use of thiol antioxidants Exp. Gerontol. 37 2002 1333 1345
13. G. Ellman Tissue sulfhydryl groups Arch. Biochem. Biophys. 82 1959 70 77
14. M.S. Paget Thiol-based regulatory switches Annu. Rev. Genet. 37 2003 91 121
15. M. Unlu, M.E. Morgan, and J.S. Minden Difference gel electrophoresis: a single gel method for detecting changes in protein extracts Electrophoresis 18 1997 2071 2077
16. J. Shaw Evaluation of saturation labelling two-dimensional difference gel electrophoresis fluorescent dyes Proteomics 3 2003 1181 1195
17. G.J. Babu Solubilization of membrane proteins for two-dimensional gel electrophoresis: identification of sarcoplasmic reticulum membrane proteins Anal. Biochem. 325 2004 121 125
18. I.M. Riederer Serial protein labeling with infrared maleimide dyes to identify cysteine modifications J. Proteomics 71 2008 222 230
19. R. Thierry Membrane proteins and proteomics: love is possible, but so difficult Electrophoresis 30 2009 S174 S180
20. R. Rossi Blood glutathione disulfide: in vivo factor or in vitro artifact Clin. Chem. 48 2002 742 753
21. L. Rodgers, B. Leinweber, and C. Smith Detection of reversible protein thiol modifications in tissues Anal. Biochem. 358 2006 171 184
22. C.L. Hawkins, P.E. Morgan, and M.J. Davies Quantification of protein modification by oxidants Free Radic. Biol. Med. 46 2009 965 988
23. P.W. Riddles Reassessment of Ellman's reagent Methods Enzymol. 91 1983 49 60
24. C.K. Riener Quick measurement of protein sulfhydryls with Ellman's reagent and with 4, 4′-dithiodipyridine Anal. Bioanal. Chem. 373 2002 266 276
25. P. Eyer Molar absorption coefficients for the reduced Ellman reagent: reassessment Anal. Biochem. 312 2003 224 227
26. T.A. Kohn Electrophoretic separation of human skeletal muscle myosin heavy chain isoforms: the importance of reducing agents J. Physiol. Sci. 56 2006 355 360
27. L.I. Leichert Quantifying changes in the thiol redox proteome upon oxidative stress in vivo Proc. Natl Acad. Sci. USA 105 2008 8197 8202
28. T.A. Rando Muscle cells from mdx mice have an increased susceptibility to oxidative stress Neuromusc. Disord. 8 1998 14 21
29. M.-H. Disatnik Evidence of oxidative stress in mdx mouse muscle: studies of the pre-necrotic state J. Neurol. Sci. 161 1998 77 84
30. Passonneau J.V., and Lowry O.H. Enzymatic Analysis: a Practical Guide 1993 Humana Press Totowa, NJ
31. E. Burmeister A comparison between the sulfhydryl reductants tris(2-carboxyethyl) phosphine and dithiothreitol for use in protein biochemistry Anal. Biochem. 273 1999 73 80
32. M.L. Freeman, and M.J. Meredith Measurement of protein thiols after heat shock using 3-(N-maleimido-propionyl) biocytin labeled proteins separated by SDS-PAGE and electroeluted onto nitrocellulose: thiol blotting Radiat. Res. 117 1989 326 333
33. F. Lucesoli Dose-dependent increase of oxidative damage in the testes of rats subjected to acute iron overload Arch. Biochem. Biophys. 372 1999 37 43
34. A. Balcerczyk Thiols as major determinants of the total antioxidant capacity Biofactors 17 2003 75 82
35. M. Varsanyi FAD transport and FAD-dependent protein thiol oxidation in rat liver microsomes J. Biol. Chem. 279 2004 3370 3374
36. M. Prakash, S. Upadhya, and R. Prabhu Protein thiol oxidation and lipid peroxidation in patients with uraemia Scand. J. Clin. Lab. Invest. 64 2004 599 604
37. J.K. Lui Detecting changes in the thiol redox state of proteins following a decrease in oxygen concentration using a dual labeling technique J. Proteome Res. 9 2010 383 392
38. I.M. Riederer Differential protein labeling with thiol-reactive infrared DY-680 and DY-780 maleimides and analysis by two-dimensional gel electrophoresis Proteomics 7 2007 1753 1756