A two-step protocol for shoot regeneration from hypocotyl explants of oilseed rape and its application for Agrobacterium-mediated transformation

Biologia Plantarum

Volumn 55, Issue 1, 2011, Pages 21-26

  Tang, G X ^a   Knecht, K ^b   Yang, X F ^a   Qin, Y B ^a   Zhou, W J ^a   Cai, D ^b  

^a ZHEJIANG UNIVERSITY   (China)

^b UNIVERSITY OF KIEL   (Germany)

Author keywords

auxins; benzylaminopurine; Brassica napus; callus induction; plant tissue culture

Indexed keywords

AGROBACTERIUM; BRASSICA NAPUS; RHIZOBIUM;

EID: 79251592903     PISSN: 00063134     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10535-011-0003-0     Document Type: Article

References (28)

1. 3 and genotype. - Plant Cell Rep. 24: 649-654, 2005.
2. Cardoza, V., Stewart, C. N.: Increased Agrobacterium-mediated transformation and rooting efficiencies in canola (Brassica napus L.) from hypocotyl segment explants. - Plant Cell Rep. 21: 599-604, 2003.
3. Chi, G. L., Pua, E. C., Goh, C. J.: Role of ethylene on de novo shoot regeneration from cotyledonary explants of Brassica campestris ssp. pekinensis (Lour) Olesson in vitro. - Plant Physiol. 96: 178-183, 1991.
4. Cho, M. A., Moon, C. Y., Liu, J. R., Cho, P. S.: Agrobacterium-mediated transformation in Citrullus lanatus. - Biol. Plant. 52: 365-369, 2008.
5. Dibax, R., Deschamps, C., Bespalhok Filho, J. C., Vieira, L. G. E., Molinari, H. B. C., De Campos, M. K. F., Quorin, E.: Organogenesis and Agrobacterium tumefaciens-mediated transformation of Eucalyptus saligma with P5CS gene. - Biol. Plant. 54: 6-12, 2010.
6. Feinberg, A. P., Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132: 6-13, 1983.
7. Fry, J., Barnason, A., Horsch, R. B.: Transformation of Brassica napus with Agrobacterium tumefaciens based vector. - Plant Cell Rep. 6: 321-325, 1987.
8. Ghnaya, A. B., Charles, G.: Rapid shoot regeneration from thin cell layer explants excised from petioles and hypocotyls in four cultivars of Brassica napus L. - Plant Cell Tissue Organ Cult. 92: 25-30, 2008.
9. Hansen, G., Wright, M. S.: Recent advances in the transformation of plants. - Trends Plant Sci. 4: 226-231, 1999.
10. Jonoubi, P., Mousavi, A., Majd, A., Salmanian, A. H., Javaran M. J., Daneshian, J.: Efficient regeneration of Brassica napus L. hypocotyls and genetic transformation by Agrobacterium tumefaciens. - Biol. Plant. 49: 175-180, 2005.
11. Khehra, G. S., Mathias, R. J.: The interaction of genotype, explant and media on the regeneration of shoots from complex explants of Brassica napus. - J. exp. Bot. 43: 1413-1418, 1992.
12. Khan, R. M., Rashid, H., Muhammad, A., Chandry, Z.: High frequency shoot regeneration and Agrobacterium-mediated DNA transfer in canola (Brassica napus). - Plant Cell Tissue Organ Cult. 75: 223-231, 2003.
13. Klimaszewska, K., Keller, W. A.: High frequency plant regeneration from thin cell layer explants of Brassica napus. - Plant Cell Tissue Organ Cult. 4: 183-197, 1985.
14. Lee, Y. H., Kim, H. S., Kim, J. Y., Jung, M., Park, Y. S., Lee, J. S., Choi, S. H., Her, N. H., Lee, J. H., Hyung, N. I., Lee, C. H., Yang, S. G., Harn, C. H.: A new selection method for pepper transformation: callus-mediated shoot formation. - Plant Cell Rep. 23: 50-58, 2004.
15. Lein, J. C., Asbach K., Tian, Y., Schulte, D., Li, C., Koch, G., Jung, C., Cai, D.: Resistance gene analogues are clustered on chromosome 3 of sugar beet and cosegregate with QTL for rhizomania resistance. - Genome 50: 61-71, 2007.
16. Mukhopadhyay, A., Arumugam, N., Nandakumar, P. B. A., Pradhan, A. K., Gupta, V., Pental, D.: Agrobacterium- mediated genetic transformation of oilseed Brassica campestris: transformation frequency is strongly influenced by the mode of shoot regeneration. - Plant Cell Rep. 11: 506-513, 1992.
17. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays in tobacco tissue culture. - Physiol. Plant. 15: 474-493, 1962.
18. Narasimhulu, S. B., Chopra, V. L.: Species specific shoot regeneration response of cotyledonary explants of Brassica. - Plant Cell Rep. 7: 104-106, 1988.
19. O'Neill, C. M., Arthur, A. E., Mathias, R. J.: The effects of proline, thioproline and methyglyoxal-bis-(guanylhydrazone) on shoot regeneration frequencies from stem explants of B. napus. - Plant Cell Rep. 15: 695-698, 1996.
20. Pua, E. C., Chi, G. L.: De novo shoot morphogensis and plant growth of mustard (Brassica juncea) in vitro in relation to ethylene. - Physiol. Plant. 88: 467-474, 1993.
21. Rogers, S. O., Bendich, A. J.: Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. - Plant mol. Biol. 5: 69-76, 1985.
22. Shen, W. J., Forde, B. G.: Efficient transformation of Agrobacterium spp. by high voltage electroporation. - Nucl. Acids Res. 17: 8385, 1989.
23. Shu, W., Loh, C. S.: Secondary embryogenesis from thin cell layers of Brassica napus ssp. oleifera. - New Phytol. 119: 427-432, 1991.
24. Sparrow, P. A. C., Dale, P. J., Irwin, J. A.: The use of phenotypic markers to identify Brassica oleracea genotypes for routine high throughput Agrobacterium-mediated transformation. - Plant Cell Rep. 23: 64-70, 2004.
25. Tang, H., Xiao, X. G., Chen, J.: [High frequency plant regeneration of Brassica campestris ssp. chinensis from cotyledon segments.] - J. Agr. Biotechnol. 7: 187-191, 1999. [In Chinese].
26. Tang, G. X., Zhou, W. J., Li, H. Z., Mao, B. Z., He, Z. H., Yoneyama, K.: Medium, explant and genotype factors influencing shoot regeneration in oilseed Brassica spp. - J. Agron. Crop Sci. 189: 351-358, 2003.
27. Tian, Y., Fan, L. J., Thurau, T., Jung, C., Cai, D.: The absence of TIR type resistance gene analogues in the sugar beet (Beta vulgaris L.) genome. - J. mol. Evol. 58: 40-53, 2004.
28. Turgut, K., Barghchi, M., Scott, R.: Efficient shoot regeneration and somatic embryogenesis from immature cotyledons of Brassica napus L. - Plant Breed. 117: 503-504, 1998.