Scaffold-free 3D cellulose acetate membrane-based cultures form large cartilaginous constructs

Journal of Tissue Engineering and Regenerative Medicine

Volumn 5, Issue 2, 2011, Pages 151-155

  Mayer Wagner, S ^a   Schiergens, T S ^a   Sievers, B ^a   Redeker, J I ^a   Schmitt, B ^a   Buettner, A ^b   Jansson, V ^a   Mukller, P E ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

Chondrocyte; Membrane based culture; Redifferentiation; Scaffold free

Indexed keywords

BODY FLUIDS; CARTILAGE; CELLULOSE; COLLAGEN; MEMBRANES; PELLETIZING;

CARTILAGE REGENERATION; CELLULOSE ACETATE MEMBRANE; CHONDROCYTES; CULTURE SYSTEMS; MEMBRANE-BASED; MEMBRANE-BASED CULTURE; MONOLAYER CULTURE; REDIFFERENTIATION; SCAFFOLD-FREE; TYPE II;

MONOLAYERS;

CELLULOSE ACETATE; COLLAGEN TYPE 2; TOLONIUM CHLORIDE;

ARTICLE; CARTILAGE CELL; CARTILAGINOUS TISSUE; CELL DIFFERENTIATION; CONTROLLED STUDY; HISTOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MEMBRANE FILTER; MONOLAYER CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; SURFACE PROPERTY; THICKNESS; THREE DIMENSIONAL IMAGING; TISSUE REGENERATION;

EID: 79251494772     PISSN: 19326254     EISSN: 19327005     Source Type: Journal    
DOI: 10.1002/term.300     Document Type: Article

References (18)

1. Barbero A, Grogan SP, Mainil-Varlet P, et al. 2006; Expansion on specific substrates regulates the phenotype and differentiation capacity of human articular chondrocytes. J Cell Biochem 98: 1140-1149.
2. Kruse FE, Schmitz W, Jaeger W, et al. 1986; [Scanning electron microscopy image of the cells of the conjunctival epithelium in specimens from impression cytology]. Klin Monatsbl Augenheilkd 188: 29-32.
3. Lennon DP, Edmison JM, Caplan AI. 2001; Cultivation of rat marrow-derived mesenchymal stem cells in reduced oxygen tension: effects on in vitro and in vivo osteochondrogenesis. J Cell Physiol 187: 345-355.
4. Li WJ, Chiang H, Kuo TF, et al. 2009; Evaluation of articular cartilage repair using biodegradable nanofibrous scaffolds in a swine model: a pilot study. J Tissue Eng Regen Med 3: 1-10.
5. Malpeli M, Randazzo N, Cancedda R, et al. 2004; Serum-free growth medium sustains commitment of human articular chondrocyte through maintenance of Sox9 expression. Tissue Eng 10: 145-155.
6. Martinez I, Elvenes J, Olsen R, et al. 2008; Redifferentiation of in vitro expanded adult articular chondrocytes by combining the hanging-drop cultivation method with hypoxic environment. Cell Transpl 17: 987-996.
7. Mayer S, Schiergens TS, Sievers B, et al. 2009; Membrane-based cultures generate scaffold-free neocartilage in vitro: influence of growth factors. Tissue Eng A 16(2): 513-521.
8. Mironov V, Visconti RP, Kasyanov V, et al. 2009; Organ printing: tissue spheroids as building blocks. Biomaterials 30: 2164-2174.
9. Naumann A, Dennis JE, Aigner J, et al. 2004; Tissue engineering of autologous cartilage grafts in three-dimensional in vitro macroaggregate culture system. Tissue Eng 10: 1695-1706.
10. Peterson L, Minas T, Brittberg M, et al. 2003; Treatment of osteochondritis dissecans of the knee with autologous chondrocyte transplantation: results at 2-10 years. J Bone Joint Surg Am 85A(suppl 2): 17-24.
11. Rai MF, Rachakonda PS, Manning K, et al. 2009; Molecular and phenotypic modulations of primary and immortalized canine chondrocytes in different culture systems. Res Vet Sci 87(3): 399-407.
12. Ruano-Ravina A, Jato DM. 2006; Autologous chondrocyte implantation: a systematic review. Osteoarthr Cartilage 14: 47-51.
13. Schulze-Tanzil G, de Souza P, Villegas CH, et al. 2002; Redifferentiation of dedifferentiated human chondrocytes in high-density cultures. Cell Tissue Res 308: 371-379.
14. Stewart MC, Saunders KM, Burton-Wurster N, et al. 2000; Phenotypic stability of articular chondrocytes in vitro: the effects of culture models, bone morphogenetic protein 2, and serum supplementation. J Bone Miner Res 15: 166-174.
15. Trowell OA. 1954; A modified technique for organ culture in vitro. Exp Cell Res 6: 246-248.
16. von der Mark K, Gauss V, von der Mark H, et al. 1977; Relationship between cell shape and type of collagen synthesized as chondrocytes lose their cartilage phenotype in culture. Nature 267: 531-532.
17. Xu Y, Malladi P, Chiou M, et al. 2007; In vitro expansion of adipose-derived adult stromal cells in hypoxia enhances early chondrogenesis. Tissue Eng 13: 2981-2993.
18. Zhang Z, McCaffery JM, Spencer RG, et al. 2004; Hyaline cartilage engineered by chondrocytes in pellet culture: histological, immunohistochemical and ultrastructural analysis in comparison with cartilage explants. J Anat 205: 229-237.