Flow cytometry rapidly identifies all acute promyelocytic leukemias with high specificity independent of underlying cytogenetic abnormalities

American Journal of Clinical Pathology

Volumn 135, Issue 1, 2011, Pages 76-84

  Dong, Henry Y ^a   Kung, Jia Xue ^a   Bhardwaj, Vatsala ^a   McGill, John ^b  

^a Genzyme Genetics   (United States)

^b GENZYME GENETICS   (United States)

Author keywords

Acute promyelocytic leukemia; CD117; CD11b; CD11c; Cytogenetics; Flow cytometry; HLA DR

Indexed keywords

CD11B ANTIGEN; CD34 ANTIGEN; GLYCOPROTEIN P 15095; HLA DR ANTIGEN;

ARTICLE; CANCER DIAGNOSIS; CYTOGENETICS; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; GRANULOCYTE; HUMAN; IMMUNOPHENOTYPING; KARYOTYPE; MAJOR CLINICAL STUDY; MYELOBLAST; PHENOTYPE; PREDICTOR VARIABLE; PRIORITY JOURNAL; PROMYELOCYTIC LEUKEMIA; PROTEIN EXPRESSION; SENSITIVITY AND SPECIFICITY;

ANTIGENS, CD; CHROMOSOME ABERRATIONS; FLOW CYTOMETRY; GRANULOCYTE PRECURSOR CELLS; GRANULOCYTES; HLA-DR ANTIGENS; HUMANS; IMMUNOPHENOTYPING; IN SITU HYBRIDIZATION, FLUORESCENCE; LEUKEMIA, PROMYELOCYTIC, ACUTE; MEMBRANE GLYCOPROTEINS; PREDICTIVE VALUE OF TESTS; TRANSLOCATION, GENETIC; TUMOR MARKERS, BIOLOGICAL;

EID: 79251489461     PISSN: 00029173     EISSN: 19437722     Source Type: Journal    
DOI: 10.1309/AJCPW9TSLQNCZAVT     Document Type: Article

References (43)

1. Swerdlow SH, Campo E, Harris NL, et al. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. 4th ed. Lyon, France: IARC Press; 2008.
2. Ribeiro RC, Rego E. Management of APL in developing countries: epidemiology, challenges and opportunities for international collaboration. Hematology Am Soc Hematol Educ Program. 2006:162-168.
3. Stein E, McMahon B, Kwaan H, et al. The coagulopathy of acute promyelocytic leukaemia revisited. Best Pract Res Clin Haematol. 2009;22:153-163.
4. Di Noto R, Mirabelli P, Del Vecchio L. Flow cytometry analysis of acute promyelocytic leukemia: the power of "surface hematology." Leukemia. 2007;21:4-8.
5. Piedras J, Lopez-Karpovitch X, Cardenas R. Light scatter and immunophenotypic characteristics of blast cells in typical acute promyelocytic leukemia and its variant. Cytometry. 1998;32:286-290.
6. San Miguel JF, Gonzalez M, Canizo MC, et al. Surface marker analysis in acute myeloid leukaemia and correlation with FAB classification. Br J Haematol. 1986;64:547-560.
7. Hanson CA, Gajl-Peczalska KJ, Parkin JL, et al. Immunophenotyping of acute myeloid leukemia using monoclonal antibodies and the alkaline phosphatase-antialkaline phosphatase technique. Blood. 1987;70:83-89.
8. De Rossi G, Avvisati G, Coluzzi S, et al. Immunological definition of acute promyelocytic leukemia (FAB M3): a study of 39 cases. Eur J Haematol. 1990;45:168-171.
9. Paietta E, Andersen J, Gallagher R, et al. The immunophenotype of acute promyelocytic leukemia (APL): an ECOG study. Leukemia. 1994;8:1108-1112. (Pubitemid 24224511)
10. Di Noto R, Lo Pardo C, Schiavone EM, et al. Stem cell factor receptor (c-kit, CD117) is expressed on blast cells from most immature types of acute myeloid malignancies but is also a characteristic of a subset of acute promyelocytic leukaemia. Br J Haematol. 1996;92:562-564.
11. Guglielmi C, Martelli MP, Diverio D, et al. Immunophenotype of adult and childhood acute promyelocytic leukaemia: correlation with morphology, type of PML gene breakpoint and clinical outcome: a cooperative Italian study on 196 cases. Br J Haematol. 1998;102:1035-1041.
12. Orfao A, Chillon MC, Bortoluci AM, et al. The flow cytometric pattern of CD34, CD15 and CD13 expression in acute myeloblastic leukemia is highly characteristic of the presence of PML-RARalpha gene rearrangements. Haematologica. 1999;84:405-412.
13. Rizzatti EG, Garcia AB, Portieres FL, et al. Expression of CD117 and CD11b in bone marrow can differentiate acute promyelocytic leukemia from recovering benign myeloid proliferation. Am J Clin Pathol. 2002;118:31-37.
14. Haferlach T, Loffler H, Nickenig C, et al. Cell lineage specific involvement in acute promyelocytic leukaemia (APL) using a combination of May-Grünwald-Giemsa staining and fluorescence in situ hybridization techniques for the detection of the translocation t(15;17)(q22;q12). Br J Haematol. 1998;103:93-99. (Pubitemid 28470805)
15. Kaleem Z, Crawford E, Pathan MH, et al. Flow cytometric analysis of acute leukemias: diagnostic utility and critical analysis of data. Arch Pathol Lab Med. 2003;127:42-48. (Pubitemid 36236578)
16. Lin P, Hao S, Medeiros LJ, et al. Expression of CD2 in acute promyelocytic leukemia correlates with short form of PML-RARα transcripts and poorer prognosis. Am J Clin Pathol. 2004;121:402-407. (Pubitemid 38298174)
17. Paietta E, Goloubeva O, Neuberg D, et al. A surrogate marker profile for PML/RAR alpha expressing acute promyelocytic leukemia and the association of immunophenotypic markers with morphologic and molecular subtypes. Cytometry B Clin Cytom. 2004;59:1-9.
18. Di Noto R, Schiavone EM, Ferrara F, et al. Expression and ATRA-driven modulation of adhesion molecules in acute promyelocytic leukemia. Leukemia. 1994;8:1900-1905.
19. Stasi R, Bruno A, Venditti A, et al. A microgranular variant of acute promyelocytic leukemia with atypical morpho-cytochemical features and an early myeloid immunophenotype. Leuk Res. 1997;21:575-580.
20. Erber WN, Asbahr H, Rule SA, et al. Unique immunopheno-type of acute promyelocytic leukaemia as defined by CD9 and CD68 antibodies. Br J Haematol. 1994;88:101-104.
21. Murray CK, Estey E, Paietta E, et al. CD56 expression in acute promyelocytic leukemia: a possible indicator of poor treatment outcome? J Clin Oncol. 1999;17:293-297.
22. Edwards RH, Wasik MA, Finan J, et al. Evidence for early hematopoietic progenitor cell involvement in acute promyelocytic leukemia. Am J Clin Pathol. 1999;112:819-827. (Pubitemid 30212513)
23. Exner M, Thalhammer R, Kapiotis S, et al. The "typical" immunophenotype of acute promyelocytic leukemia (APL-M3): does it prove true for the M3-variant? Cytometry. 2000;42:106-109.
24. Foley R, Soamboonsrup P, Carter RF, et al. CD34-positive acute promyelocytic leukemia is associated with leukocytosis, microgranular/ hypogranular morphology, expression of CD2 and bcr3 isoform. Am J Hematol. 2001;67:34-41.
25. Lee JJ, Cho D, Chung IJ, et al. CD34 expression is associated with poor clinical outcome in patients with acute promyelocytic leukemia. Am J Hematol. 2003;73:149-153. (Pubitemid 36753955)
26. Scott AA, Head DR, Kopecky KJ, et al. HLA-DR-, CD33+, CD56+, CD16- myeloid/natural killer cell acute leukemia: a previously unrecognized form of acute leukemia potentially misdiagnosed as French-American-British acute myeloid leukemia-M3. Blood. 1994;84:244-255.
27. Lazarchick J, Hopkins M. HLA-DR negative acute non-lymphocytic leukemia. Ann Clin Lab Sci. 1998;28:150-152.
28. Wetzler M, McElwain BK, Stewart CC, et al. HLA-DR antigen-negative acute myeloid leukemia. Leukemia. 2003;17:707-715. (Pubitemid 36519367)
29. Shen HQ, Tang YM, Song H, et al. Expressions of CD117 and CD11b in patients with APL at diagnosis and post-treatment [in Chinese]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006;14:644-648.
30. Paietta E. Expression of cell-surface antigens in acute promyelocytic leukaemia. Best Pract Res Clin Haematol. 2003;16:369-385.
31. Davis BH, Holden JT, Bene MC, et al. 2006 Bethesda International Consensus recommendations on the flow cytometric immunophenotypic analysis of hematolymphoid neoplasia: medical indications. Cytometry B Clin Cytom. 2007;72(suppl 1):S5-S13.
32. Choppa PC, Gomez J, Vall HG, et al. A novel method for the detection, quantitation, and breakpoint cluster region determination of t(15;17) fusion transcripts using a one-step real-time multiplex RT-PCR. Am J Clin Pathol. 2003;119:137-144.
33. Grimwade D, Biondi A, Mozziconacci MJ, et al; for the Groupe Français de Cytogénétique Hématologique, Groupe de Français d'Hématologie Cellulaire, UK Cancer Cytogenetics Group and BIOMED 1 European Community-Concerted Action "Molecular Cytogenetic Diagnosis in Haematological Malignancies." Characterization of acute promyelocytic leukemia cases lacking the classic t(15;17): results of the European Working Party. Blood. 2000;96:1297-1308.
34. Lee GY, Christina S, Tien SL, et al. Acute promyelocytic leukemia with PML-RARA fusion on i(17q) and therapy-related acute myeloid leukemia. Cancer Genet Cytogenet. 2005;159:129-136.
35. Grimwade D, Gorman P, Duprez E, et al. Characterization of cryptic rearrangements and variant translocations in acute promyelocytic leukemia. Blood. 1997;90:4876-4885.
36. Wu JJ, Cantor A, Moscinski LC. β2 Integrins are characteristically absent in acute promyelocytic leukemia and rapidly upregulated in vivo upon differentiation with all-trans retinoic acid. Leuk Res. 2007;31:49-57.
37. Fujimoto H, Sakata T, Hamaguchi Y, et al. Flow cytometric method for enumeration and classification of reactive immature granulocyte populations. Cytometry. 2000;42:371-378.
38. Mollet L, Fautrel B, Leblond V, et al. Leukemic CD3+ LGL share functional properties with their CD8+ CD57+ cell counterpart expanded after BMT. Leukemia. 1999;13:230-240.
39. Miller ML, Fishleder AJ, Tubbs RR. The expression of CD22 (Leu 14) and CD11c (LeuM5) in chronic lymphoproliferative disorders using two-color flow cytometric analysis. Am J Clin Pathol. 1991;96:100-108.
40. Moon H, Lee S, Huh J, et al. Characteristics of acute myeloid leukemia without HLA-DR expression. Korean J Lab Med. 2007;27:313-317.
41. Nagendra S, Meyerson H, Skallerud G, et al. Leukemias resembling acute promyelocytic leukemia, microgranular variant. Am J Clin Pathol. 2002;117:651-657.
42. Al-Mawali A, Gillis D, Hissaria P, et al. Incidence, sensitivity, and specificity of leukemia-associated phenotypes in acute myeloid leukemia using specific five-color multiparameter flow cytometry. Am J Clin Pathol. 2008;129:934-945.
43. Biondi A, Luciano A, Bassan R, et al. CD2 expression in acute promyelocytic leukemia is associated with microgranular morphology (FAB M3v) but not with any PML gene breakpoint. Leukemia. 1995;9:1461-1466.