Real-time reverse-transcriptase PCR for Salmonella Typhimurium detection from lettuce and tomatoes

LWT

Volumn 44, Issue 4, 2011, Pages 1088-1097

  Miller, Nathan D ^a   Davidson, P M ^a   D'Souza, Doris H ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

Author keywords

Detection; Produce; RT PCR; Salmonella

Indexed keywords

AMINO ACIDS; ELECTROPHORESIS; ERROR DETECTION; FRUITS; POLYMERASE CHAIN REACTION;

GEL ELECTROPHORESIS; INTERNAL AMPLIFICATION; PRODUCE; REACTION CONDITIONS; REVERSE TRANSCRIPTASES; RT-PCR; SALMONELLA DETECTIONS; SALMONELLA TYPHIMURIUM;

SALMONELLA;

BACTERIA (MICROORGANISMS); LACTUCA; LYCOPERSICON ESCULENTUM; SALMONELLA; SALMONELLA TYPHIMURIUM;

EID: 78751706525     PISSN: 00236438     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.lwt.2010.08.003     Document Type: Article

References (31)

1. Baumler A.J., Tsolis R.M., Ficht T.A., Adams L.G. Evolution of host adaptation in Salmonella enterica. Infection and Immunity 1998, 66:4579-4587.
2. CDC Outbreak of Salmonella serotype saintpaul infections associated with multiple raw produce items-United States, 2008. Morbidity and Mortality Weekly Report 2008, 57(34):929-934.
3. CDC Salmonella outbreak investigations 2009, Available at, Accessed 2009. http://www.cdc.gov/salmonella/outbreaks.html.
4. Chiang Y.C., Fan C.M., Liao W.W., Lin C.K., Tsen H.Y. Real-time PCR detection of Staphylococcus aureus in milk and meat using new primers designed from the heat shock protein gene htrA sequence. Journal of Food Protection 2007, 70:2855-2859.
5. D'Aoust J.-Y., Maurer J. Salmonella species. Food microbiology: Fundamentals and frontiers 2007, 187-236. ASM Press, Washington, D.C. 3rd ed. M.P. Doyle, L.R. Beuchat (Eds.).
6. D'Souza D.H., Critzer F.J., Golden D.A. Real-time reverse-transcriptase-PCR (RT-PCR) for the rapid detection of Salmonella using invA primers. Foodborne Pathogens and Disease 2009, 6(9):1097-1106.
7. De Medici D., Croci L., Delibato E., Di Pasquale S., Filetici E., Toti L., et al. Evaluation of DNA extraction methods for use in combination with SYBR Green I real-time PCR to detect Salmonella enterica serotype enteritidis in poultry. Applied and Environmental Microbiology 2003, 69:3456-3461.
8. Feng P. Rapid methods for the detection of foodborne pathogens: current and next-generation technologies. Food microbiology: Fundamentals and frontiers 2007, 911-934. ASM Press, Washington, D.C. 3rd ed. M.P. Doyle, L.R. Beuchat (Eds.).
9. Fey A., Eichler S., Flavier S., Christen R., Höfle M.G., Guzmán C.A., et al. Establishment of a real-time PCR-based approach for accurate quantification of bacterial RNA targets in water, using Salmonella as a model organism. Applied and Environmental Microbiology 2004, 70:3618-3623.
10. Gonzalez-Escalona N., Hammack T.S., Russell M., Jacobson A.P., De Jesús A.J., Brown E.W., et al. Detection of live Salmonella sp. cells in produce by a TaqMan-based quantitative reverse-transcriptase real-time PCR targeting invA mRNA. Applied and Environmental Microbiology 2009, 75(11):3714-3720.
11. Guo X., Chen J., Beuchat L.R., Brackett R.E. PCR detection of Salmonella enterica serotype Montevideo in and on raw tomatoes using primers derived from hilA. Applied and Environmental Microbiology 2000, 66(12):5248-5252.
12. Harper L.V., Hilton A.C., Jones A.F. RT-PCR for the pseudogene-free amplification of the glyceraldehyde-3-phosphate dehydrogenase gene (gapd). Molecular and Cellular Probes 2003, 17:261-265.
13. Hoorfar J., Malorny B., Abdulmawjood A., Cook N., Wagner M., Fach P., et al. Practical considerations in design of internal amplification controls for diagnostic PCR assays. Journal of Clinical Microbiology 2004, 42:1863-1868.
14. Iturriaga M.H., Escartin E.F., Beuchat L.R., Martinez-Peniche R. Effect of inoculum size, relative humidity, storage temperature, and ripening stage on the attachment of Salmonella Montevideo to tomatoes and tomatillos. Journal of Food Protection 2003, 66:1756-1761.
15. Jacobsen C.S., Holben W.E. Quantification of mRNA in Salmonella sp. seeded soil and chicken manure using magnetic capture hybridization RT-PCR. Journal of Microbiological Methods 2007, 69:315-321.
16. Lang M.M., Harris L.J., Beuchat L.R. Evaluation of inoculation method and inoculum drying time for their effects on survival and efficiency of recovery of Escherichia coli O157: H7, Salmonella, and Listeria monocytogenes inoculated on the surface of tomatoes. Journal of Food Protection 2004, 67:732-741.
17. Lee M.D., Fairchild A. Sample preparation for PCR. PCR methods in foods 2006, 41-50. Springer Science+ Business Media, Inc., New York, NY. J. Maurer (Ed.).
18. Maurer J.J. The mythology of PCR: a warning to the wise. PCR methods in foods 2006, 27-40. Springer Science+ Business Media, Inc., New York, NY. J. Maurer (Ed.).
19. Mercanoglu B., Griffiths M.W. Combination of immunomagnetic separation with real-time PCR for rapid detection of Salmonella in milk, ground beef, and alfalfa sprouts. Journal of Food Protection 2005, 68:557-561.
20. Miller N.D., Draughon F.A., D'Souza D.H. Real-time reverse-transcriptase-polymerase chain reaction for Salmonella enterica detection from jalapeno and serrano peppers. Foodborne Pathogens and Disease 2010, 7(4):367-373.
21. Moreira A.N., Conceicao F.R., Conceicao R.D.S., Dias C.N., Carvalhal J.B., Dellagostin O.A., et al. IMS using in-house monoclonal anitbody-coated magnetic beads associated to PCR assay for detection of Salmonella Typhimurium in raw meats. Journal of Food Safety 2009, 29:59-72.
22. Perera K., Murray A. Development of a PCR assay for the identification of Salmonella enterica serovar Brandenburg. Journal of Medical Microbiology 2008, 57:1223-1227.
23. Rahn K., Degrandis S.A., Clarke R.C., McEwen S.A., Galan G.E., Ginocchio C., et al. Amplification of an invA gene sequence of Salmonella Typhimurium by polymerase chain-reactin as a specific method of detection of Salmonella. Molecular and Cellular Probes 1992, 6:271-279.
24. Shi X., Namvar A., Kostrzynska M., Hora R., Warriner K. Persistence and growth of different Salmonella serovars on pre- and postharvest tomatoes. Journal of Food Protection 2007, 70:2725-2731.
25. Sivapalasingam S., Friedman C.R., Cohen L., Tauxe R.V. Fresh produce: a growing cause of outbreaks of foodborne illness in the United States, 1973 through 1997. Journal of Food Protection 2004, 67:2342-2353.
26. Szabo E.A., Mackey B.M. Detection of Salmonella enteritidis by reverse transcription-polymerase chain reaction (PCR). International Journal of Food Microbiology 1999, 51:113-122.
27. Techathuvanan C., Draughon F.A., D'Souza D.H. Real-time reverse-transcriptase-PCR for the rapid and sensitive detection of Salmonella from pork. Journal of Food Protection 2010, 73(3):507-514.
28. Wang H., Slavik M.F. A multiplex polymerase chain reaction assay for rapid detection of Escherichia coli O157: H7, Listeria monocytogenes, Salmonella Typhimurium and Campylobacter jejuni in artificially contaminated food samples. Journal of Rapid Methods and Automation in Microbiology 2005, 13:213-223.
29. Warren B.R., Yuk H.G., Schneider K.R. Detection of Salmonella by flow-through immunocapture real-time PCR in selected foods within 8 hours. Journal of Food Protection 2007, 70:1002-1006.
30. Werbrouck H., Botteldoorn N., Uyttendaele M., Herman L., Van Coillie E. Quantification of gene expression of Listeria monocytogenes by real-time reverse transcription PCR: optimization, evaluation and pitfalls. Journal of Microbiological Methods 2007, 69:306-314.
31. Yuk H.G., Warren B.R., Schneider K.R. Preliminary evaluation of flow-through immunocapture followed by real-time PCR for the detection of Salmonella serovars on tomato surfaces within 8 hours. Journal of Food Protection 2006, 69:2253-2257.