Quantitative analysis of photosynthetic carbon metabolism in protoplasts and intact leaves of barley. Determination of carbon fluxes and pool sizes of metabolites in different cellular compartments

BioSystems

Volumn 103, Issue 2, 2011, Pages 291-301

  Keerberg, Olav ^a   Ivanova, Hiie ^a   Keerberg, Hille ^a   Pärnik, Tiit ^a   Talts, Peeter ^a   Gardeström, Per ^b  

^a ESTONIAN UNIVERSITY OF LIFE SCIENCES   (Estonia)

^b UMEÅ UNIVERSITY   (Sweden)

Author keywords

Carbon fluxes; Cellular compartments; Labeling functions; Photosynthetic carbon metabolism; Pool sizes of metabolites; Protoplasts of barley

Indexed keywords

ALANINE; ASPARTIC ACID; CARBON; CARBON 14; GLUTAMIC ACID; MALIC ACID; STARCH; SUCROSE; SUGAR PHOSPHATE; TRACER;

BARLEY; CARBON FLUX; CHLOROPLAST; FILTRATION; METABOLISM; METABOLITE; MITOCHONDRION; PHOTOSYNTHESIS; PHYSIOLOGICAL RESPONSE; QUANTITATIVE ANALYSIS; REGRESSION ANALYSIS;

AFFINITY LABELING; ARTICLE; BARLEY; CARBON DYNAMICS; CARBON METABOLISM; CARBON PARTITIONING; CELL FRACTIONATION; CELLULAR DISTRIBUTION; CHLOROPLAST; CONTROLLED STUDY; CYTOSOL; FRACTIONATION; INTRACELLULAR SPACE; ION CURRENT; KINETICS; LINEAR REGRESSION ANALYSIS; MEMBRANE FILTER; METABOLITE; MITOCHONDRION; NATURAL RADIOACTIVITY; NONHUMAN; PHOTOSYNTHESIS; PLANT LEAF; PROTOPLAST; QUANTITATIVE ANALYSIS; STEADY STATE;

HORDEUM;

EID: 78751705427     PISSN: 03032647     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biosystems.2010.10.012     Document Type: Article

References (39)

1. Anderson L.E., Goldhaber-Gordon I.M., Li D., Tang X., Xiang M., Prakash N. Enzyme-enzyme interaction in the chloroplast, glyceraldehyde-3-phosphate dehydrogenase, triose phosphate isomerase and aldolase. Planta 1995, 196:245-255.
2. Anderson L.E., Carol A.A. Enzyme co-localization with Rubisco in pea leaf chloroplasts. Photosynth. Res. 2004, 82:49-58.
3. Anderson L.E., Gatla N., Carol A.A. Enzyme co-localization in pea leaf chloroplasts, glyceraldehyde-3-P dehydrogenase, triose-P isomerase, aldolase and sedoheptulose bisphosphatase. Photosynth. Res. 2005, 83:317-328.
4. Arnon D.I. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 1949, 24:1-15.
5. Bassham J.A., Benson A.A., Kay L.D., Harris A.Z., Wilson A.T., Calvin M. The path of carbon in photosynthesis. XXI. The cyclic regeneration of carbon dioxide acceptor. J. Am. Chem. Soc. 1954, 76:1760-1770.
6. Bowsher C.G., Tobin A.K. Compartmentation of metabolism within mitochondria and plastids. J. Exp. Bot. 2001, 52:513-527.
7. Flügge I.U., Woo K.C., Heldt H.W. Characteristics of 2-oxoglutarate and glutamate transport in spinach chloroplasts. Planta 1988, 174:534-541.
8. Gardeström P., Wigge B. Influence of photorespiration on ATP/ADP ratios in the chloroplasts, mitochondria, and cytosol, studied by rapid fractionation of barley (Hordeum vulgare) protoplasts. Plant Physiol. 1988, 88:69-76.
9. Gardeström P. Metabolite levels in the chloroplast and extrachloroplast compartments of barley leaf protoplasts during the initial phase of photosynthetic induction. Biochim. Biophys. Acta 1993, 1183:327-332.
10. Gerhardt R., Heldt H.W. Measurement of subcellular metabolite levels in leaves by fractionation of freeze-stopped material in nonaqueous media. Plant Physiol. 1984, 75:542-547.
11. Gerhardt R., Stitt M., Heldt H.W. Subcellular metabolite levels in spinach leaves. Regulation of sucrose synthesis during diurnal alterations in photosynthetic partitioning. Plant Physiol. 1987, 83:399-407.
12. Giersch C., Heber U., Kaiser G., Walker D.A., Robinson S.P. Intracellular metabolite gradients and flow of carbon during photosynthesis of leaf protoplasts. Arch. Biochem. Biophys. 1980, 205:246-259.
13. Gimmler H., Schäfer G., Kraminer H., Heber U. Amino acid permeability of the chloroplast envelope as measured by light scattering, volumetry and amino acid uptake. Planta 1974, 120:47-61.
14. Igamberdiev A., Krömer S., Hurry V.M., Gardeström P. The role of mitochondrial electron transport during photosynthetic induction. A study with barley (Hordeum vulgare) protoplasts incubated with rotenone and oligomycin. Physiol. Plant. 1998, 104:431-439.
15. Igamberdiev A.U., Gardeström P. Regulation of NAD- and NADP-dependent isocitrate dehydrogenase by reduction levels of pyridine nucleotides in mitochondria and cytosol of pea leaves. Biochim. Biophys. Acta 2003, 1606:117-125.
16. Igamberdiev A.U., Chen T., Gardeström P. Function of mitochondria during the transition from low light to high light. A study with barley (Hordeum vulgare) protoplaste incubated with oligomycin and rotenone. Planta 2006, 224:196-204.
17. Keerberg O., Bassüner B. Incorporation of 14C into the products of steady state photosynthesis: analysis of the kinetics. Kinetics of Photosynthetic Carbon Metabolism in C3 Plants 1985, vol. II:124-132. Valgus, Tallinn.
18. Leidreiter K., Kruse A., Heineke D., Robinson D.G., Heldt H.W. Subcellular volumes and metabolite concentrations in potato (Solanum tuberosum cv Desiree) leaves. Bot. Acta 1995, 108:439-444.
19. Lilley R.M.C., Chon C.J., Mosbach A., Heldt H.W. The distribution of metabolites between spinach chloroplasts and medium during photosynthesis in vitro. Biochim. Biophys. Acta 1977, 460:259-272.
20. Lilley R.M.C., Stitt M., Mader G., Heldt H.W. Rapid fractionation of wheat leaf protoplasts using membrane filtration. The determination of metabolite levels in the chloroplasts, cytosol, and mitochondria. Plant Physiol. 1982, 70:965-970.
21. Lunn J.E. Compartmentation in plant metabolism. J. Exp. Bot. 2007, 58:35-47.
22. Marques I.A., Ford D.M., Muschinek G., Anderson L.E. Photosynthetic carbon metabolism in isolated pea chloroplasts: metabolite levels and enzyme activities. Arch. Biochem. Biophys. 1987, 252:458-466.
23. Pärnik T.R., Keerberg O.F., Yurisma E.Y. Fast acting exposure chamber for studying photosynthesis with 14CO2. Soviet Plant Physiol. 1987, 34:673-683.
24. Peterson R.B. Regulation of glycine decarboxylase and l-serine hydroxymethyltransferase activities by glyoxylate in tobacco leaf mitochondrial preparations. Plant Physiol. 1982, 70:61-66.
25. Riens B., Lohaus G., Heineke D., Heldt H.W. Amino acid and sucrose content determined in the cytosolic, chloroplastic, and vacuolar compartments and in the phloem sap of spinach leaves. Plant Physiol. 1991, 97:227-233.
26. Schulze-Siebert D., Heineke D., Scharf H., Schultz G. Pyruvate-derived amino acids in spinach chloroplasts. Synthesis and regulation during photosynthetic carbon metabolism. Plant Physiol. 1984, 76:465-471.
27. Stitt M., Lilley R.M.C., Heldt H.W. Adenine nucleotide levels in the cytosol, chloroplasts, and mitochondria of wheat leaf protoplasts. Plant Physiol. 1982, 70:971-977.
28. Süss K.-H., Arkona C., Manteuffel R., Adler K. Calvin cycle multienzyme complexes are bound to chloroplast thylakoid membranes of higher plants in situ. Proc. Natl. Acad. Sci. U.S.A. 1993, 90:5514-5518.
29. Szal B., Dabrowska Z., Malmberg G., Gardeström P., Rychter A.M. Changes in energy status of leaf cells as a consequence of mitochondrial genome rearrangement. Planta 2008, 227:697-706.
30. Szal B., Jastrzebska A., Kulka M., Lesniak K., Podgorska A., Pärnik T., Ivanova H., Keerberg O., Gardeström P., Rychter A.M. Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism. Planta 2010, 232:1371-1382.
31. Värk E., Keerberg O., Keerberg H., Pärnik T. Glycollate pathway of carbon assimilation in the leaves of kidney bean. Proc. Estonian Acad. Sci. Biol. 1970, 19:3-16. (in Russian).
32. Viil J., Pärnik T. Parameters of the reductive pentose phosphate cycle and of the glycolate pathway under different concentrations of oxygen. Z. Pflanzenphysiol. 1979, 95:213-225.
33. Werner-Washburne M., Keegstra K. l-Aspartate transport into pea chloroplasts. Kinetic and inhibitor evidence for multiple transport systems. Plant Physiol. 1985, 78:221-227.
34. Winkel B.S.J. Metabolic channeling in plants. Annu. Rev. Plant. Biol. 2004, 55:85-107.
35. Winter H., Lohaus G., Heldt H.W. Phloem transport of amino acids in relation to their cytosolic levels in barley leaves. Plant Physiol. 1992, 99:996-1004.
36. Winter H., Robinson D.G., Heldt H.W. Subcellular volumes and metabolite concentrations in barley leaves. Planta 1993, 191:180-190.
37. Winter H., Robinson D.G., Heldt H.W. Subcellular volumes and metabolite concentrations in spinach leaves. Planta 1994, 193:530-535.
38. Woo K.C., Flügge U.I., Heldt H.W. A two-translocator model for the transport of 2-oxoglutarate and glutamate in chloroplasts during ammonia assimilation in the light. Plant Physiol. 1987, 84:624-632.
39. Yu J., Woo K.C. Glutamine transport and the role of the glutamine translocator in chloroplasts. Plant Physiol. 1988, 88:1048-1054.