Recombinant human erythropoietin prevents motor neuron apoptosis in a rat model of cervical sub-acute spinal cord compression

Neuroscience Letters

Volumn 490, Issue 1, 2011, Pages 57-62

  Ning, Bin ^a,b   Zhang, Aijun ^a,c   Song, Hongliang ^b   Gong, Weiming ^b   Ding, Yuanjing ^b   Guo, Shuya ^b   Zhao, Yunpeng ^a   Jiang, Jianhao ^a   Jia, Tanghong ^a,b  

^a SHANDONG UNIVERSITY   (China)

^b JINAN CENTRAL HOSPITAL AFFILIATED TO SHANDONG UNIVERSITY   (China)

^c SHANDONG UNIVERSITY   (China)

Author keywords

Apoptosis; Cervical sub acute spinal cord compression; Cytokine; Recombinant human erythropoietin (rhEPO); Wistar rats

Indexed keywords

ERYTHROPOIETIN; ERYTHROPOIETIN RECEPTOR; INTERLEUKIN 1BETA; INTERLEUKIN 6; INTERLEUKIN 8; RECOMBINANT ERYTHROPOIETIN; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CERVICAL SPINAL CORD; CONTROLLED STUDY; DRUG MEGADOSE; FEMALE; FLOW CYTOMETRY; IMMUNOHISTOCHEMISTRY; LOW DRUG DOSE; MOTONEURON; MOTOR PERFORMANCE; NEUROPROTECTION; NICK END LABELING; NONHUMAN; PRIORITY JOURNAL; RAT; SPINAL CORD COMPRESSION;

ANALYSIS OF VARIANCE; ANIMALS; ANNEXIN A5; APOPTOSIS; AQUAPORINS; CERVICAL VERTEBRAE; CYTOKINES; DISEASE MODELS, ANIMAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ERYTHROPOIETIN, RECOMBINANT; EYE PROTEINS; FEMALE; FLOW CYTOMETRY; HUMANS; IN SITU NICK-END LABELING; MOTOR NEURONS; NEUROPROTECTIVE AGENTS; RANDOM ALLOCATION; RATS; RATS, WISTAR; SPINAL CORD COMPRESSION; TIME FACTORS;

EID: 78751704214     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.neulet.2010.12.025     Document Type: Article

References (13)

1. Basso D.M., Beattie M.S., Bresnahan J.C. A sensitive and reliable locomotor rating scale for open field testing in rats. J. Neurotrauma 1995, 12:1-21.
2. Brines M.L., Ghezzi P., Keenan S., Agnello D., de Lanerolle N.C., Cerami C., Itri L.M., Cerami A. Erythropoietin crosses the blood-brain barrier to protect against experimental brain injury. Proc. Natl. Acad. Sci. U.S.A. 2000, 97:10526-10531.
3. Celik M., Gokmen N., Erbayraktar S., Akhisaroglu M., Konakc S., Ulukus C., Genc S., Genc K., Sagiroglu E., Cerami A., Brines M. Erythropoietin prevents motor neuron apoptosis and neurologic disability in experimental spinal cord ischemic injury. Proc. Natl. Acad. Sci. U.S.A. 2002, 99:2258-2263.
4. Gorio A., Gokmen N., Erbayraktar S., Yilmaz O., Madaschi L., Cichetti C., Di Giulio A.M., Vardar E., Cerami A., Brines M. Recombinant human erythropoietin counteracts secondary injury and markedly enhances neurological recovery from experimental spinal cord trauma. Proc. Natl. Acad. Sci. U.S.A. 2002, 99:9450-9455.
5. Hirose K., Okajima K., Uchiba M., Nakano K.Y., Utoh J., Kitamura N. Antithrombin reduces the ischemia/reperfusion-induced spinal cord injury in rats by attenuating inflammatory responses. Thromb. Haemost. 2004, 91:162-170.
6. Houle J.D., Tessler A. Repair of chronic spinal cord injury. Exp. Neurol. 2003, 182:247-260.
7. Kaptanoglu E., Solaroglu I., Okutan O., Surucu H.S., Akbiyik F., Beskonakli E. Erythropoietin exerts neuroprotection after acute spinal cord injury in rats: effect on lipid peroxidation and early ultrastructural findings. Neurosurg. Rev. 2004, 27:113-120.
8. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951, 193:265-275.
9. Okada S., Nakamura M., Mikami Y., Shimazaki T., Mihara M., Ohsugi Y., Iwamoto Y., Yoshizaki K., Kishimoto T., Toyama Y., Okano H. Blockade of interleukin-6 receptor suppresses reactive astrogliosis and ameliorates functional recovery in experimental spinal cord injury. J. Neurosci. Res. 2004, 76:265-276.
10. Shingo T., Sorokan S.T., Shimazaki T., Weiss S. Erythropoietin regulates the in vitro and in vivo production of neuronal progenitors by mammalian forebrain neural stem cells. J. Neurosci. 2001, 21:9733-9743.
11. Uchida K., Baba H., Maezawa Y., Kubota C. Progressive changes in neurofilament proteins and growth-associated protein-43 immunoreactivities at the site of cervical spinal cord compression in spinal hyperostotic mice. Spine 2002, 27:480-486.
12. Xu P., Gong W.M., Li Y., Zhang T., Zhang K., Yin D.Z., Jia T.H. Destructive pathological changes in the rat spinal cord due to chronic mechanical compression. Lab. Invest. J. Neurosurg. Spine 2008, 8:279-285.
13. Xu X., Zhang S., Zhang L., Yan W., Zheng X. The Neuroprotection of puerarin against cerebral ischemia is associated with the prevention of apoptosis in rats. Planta Med. 2005, 71:585-591.