The morphology and composition of cholesterol-rich micellar nanostructures determine transmembrane protein (GPCR) activity

Biophysical Journal

Volumn 100, Issue 2, 2011, Pages

  O'Malley, Michelle A ^a   Helgeson, Matthew E ^b   Wagner, Norman J ^b   Robinson, Anne S ^a  

^a University of Delaware   (United States)

^b UNIVERSITY OF DELAWARE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MAMMALIA;

EID: 78751683949     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2010.12.3698     Document Type: Article

References (13)

1. Sanders, C. R., A. Kuhn Hoffmann,..., C. D. Ellis. 2004. French swimwear for membrane proteins. ChemBioChem. 5:423-426.
2. Seddon, A. M., P. Curnow, and P. J. Booth. 2004. Membrane proteins, lipids and detergents: not just a soap opera. Biochim. Biophys. Acta. 1666:105-117.
3. Chiu, M. L., C. Tsang,..., M. P. MacWilliams. 2008. Over-expression, solubilization, and purification of GPCRs for structural biology. Comb. Chem. High Throughput Screen. 11:439-462.
4. Garavito, R. M., and S. Ferguson-Miller. 2001. Detergents as tools in membrane biochemistry. J. Biol. Chem. 276:32403-32406.
5. Pucadyil, T. J., and A. Chattopadhyay. 2006. Role of cholesterol in the function and organization of G-protein coupled receptors. Prog. Lipid Res. 45:295-333.
6. Hanson, M. A., V. Cherezov,..., R. C. Stevens. 2008. A specific cholesterol binding site is established by the 2.8 A structure of the human β2-adrenergic receptor. Structure. 16:897-905.
7. Weiss, H. M., and R. Grisshammer. 2002. Purification and characterization of the human adenosine A (2a) receptor functionally expressed in Escherichia coli. Eur. J. Biochem. 269:82-92.
8. O'Malley, M. A., T. Lazarova,..., A. S. Robinson. 2007. High-level expression in Saccharomyces cerevisiae enables isolation and spectroscopic characterization of functional human adenosine A (2) a receptor. J. Struct. Biol. 159:166-178.
9. Timmins, P. A., M. Leonhard,..., W. Welte. 1988. A physical characterization of some detergents of potential use for membrane protein crystallization. FEBS Lett. 238:361-368.
10. Jaakola, V. P., M. T. Griffith,..., R. C. Stevens. 2008. The 2.6 angstrom crystal structure of a human A2A adenosine receptor bound to an antagonist. Science. 322:1211-1217.
11. Lyman, E., C. Higgs,..., G. A. Voth. 2009. A role for a specific cholesterol interaction in stabilizing the Apo configuration of the human A (2A) adenosine receptor. Structure. 17:1660-1668.
12. le Maire, M., P. Champeil, and J. V. Moller. 2000. Interaction of membrane proteins and lipids with solubilizing detergents. Biochim. Biophys. Acta. 1508:86-111.
13. Mouritsen, O. G., and M. J. Zuckermann. 2004. What's so special about cholesterol? Lipids. 39:1101-1113.