Whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of bacteria cultured in liquid media

Science China Life Sciences

Volumn 54, Issue 1, 2011, Pages 48-53

  Zhou, Na ^a   Wang, Na ^b   Xu, Bin ^b   Wang, Jie ^b   Fang, Jun Jian ^b   Dong, Fang Ting ^b   He, Kun ^b   Yang, Xiao Hong ^a  

^a JILIN UNIVERSITY   (China)

^b NATIONAL CENTER OF BIOMEDICAL ANALYSIS   (China)

Author keywords

bacteria; identification; MALDI TOF MS; PCA

Indexed keywords

EID: 78751654524     PISSN: 16747305     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11427-010-4119-9     Document Type: Article

References (17)

1. Somtcjemko V, Iredell J R, Gilbert G L. Pathogen profiling for disease management and surveillance. Nat Rev Microbiol, 2007, 5: 464-470.
2. Widjojoatmodjo M N, Fluit A C, Verhoef J. Rapid identification of bacteria by PCR-single-strand conformation polymorphism. Clin Microbiol, 1994, 32: 3002-3007.
3. Pershing D H, Tenover F C, Versalovic J, et al. Molecular microbiology: Diagnostic principles and practice, 8th ed. Washington DC: American Society for Microbiology Press, 2003.
4. Anhalt J P, Fenselau C. Identification of bacteria using mass spectrometry. Anal Chem, 1975, 47: 219-225.
5. Ryzhov V, Fenselau C. Characterization of the protein subset desorbed by MALDI from whole bacterial cells. Anal Chem, 2001, 73: 746-750.
6. Demirev P A, Ramirez J, Fenselau C. Tandem mass spectrometry of intact proteins for characterization of biomarkers from Bacillus cereus T spores. Anal Chem, 2001, 73: 5725-5731.
7. Warscheid B, Fenselau C. A targeted proteomics approach to the rapid identification of bacterial cell mixtures by matrix-assisted laser desorption/ionization mass spectrometry. Proteomics, 2004, 4: 2877-2892.
8. Pribil P A, Patton E, Black G, et al. Rapid characterization of Bacil lus spores targeting species-unique peptides produced with an atmospheric pressure matrix-assisted laser desorption/ionization source. J Mass Spectrom, 2005, 40: 464-474.
9. Sauer S, Freiwald A, Maier T, et al. Classification and identification of bacteria by mass spectrometry and computational analysis. PLoS One, 2008, 3: e2843.
10. Carbonnelle E, Beretti J L, Cottyn S, et al. Rapid identification of Staphylococci isolated in clinical microbiology laboratories by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol, 2007, 45: 2156-2161.
11. Mellmann A, Cloud J, Maier T, et al. Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria. J Clin Microbiol, 2008, 46: 1946-1954.
12. Piseth S, Michel D, Frederique G, et al. Ongoing revolution in bacteriology: Routine identification of bacteria by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis, 2009, 49: 543-551.
13. Torsten S. Whole-cell matrix-assisted laser desorption/ionization mass spectrometry for rapid identification of bacteriocin/lantibiotic-producing bacteria. Rapid Commun Mass Spectrom, 2008, 22: 1146-1152.
14. Hoben H J, Somasegaran P. Comparison of the pour, spread and drop plate methods for enumeration of Rhizobium spp. in inoculants made from presterilized peat. Appl Environ Microbial, 1982, 44: 1246-1247.
15. Song Y S, Talaty N, Tao W A, et al. Rapid ambient mass spectrometric profiling of intact, untreated bacteria using desorption electrospray ionization. Chem Commun, 2007, 1: 61-63.
16. Lindon J C, Holmes E, Nicholson J K. Metabonomics techniques and applications to pharmaceutical research & development. Pharm Res, 2006, 23: 1075-1088.
17. Hsieh S Y, Tseng C L, Lee Y S, et al. Highly efficient classification and identification of human pathogenic bacteria by MALDI-TOF MS. Mol Cell Proteomics, 2008, 7: 448-456.