Glucose-lowering activity of novel tetrasaccharide glyceroglycolipids from the fruits of Cucurbita moschata

Bioorganic and Medicinal Chemistry Letters

Volumn 21, Issue 3, 2011, Pages 1001-1003

  Jiang, Zhiguo ^a   Du, Qizhen ^a  

^a ZHEJIANG GONGSHANG UNIVERSITY   (China)

Author keywords

Cucurbita moschata; Glucose lowering; Pumpkin; Tetrasaccharide galactolipid

Indexed keywords

1 O (OCTADECADIENOYL) 3 O [BETA DEXTRO GALACTOPYRANOSYL(1-6) O BETA DEXTRO GALACTOPYRANOSYL(1-6) O BETA DEXTRO GALACTOPYRANOSYL(1-6) O BETA DEXTRO GALACTOPYRANOSYL]GLYCEROL; 1 O (OCTADECATRIENOYL) 3 O [BETA DEXTRO GALACTOPYRANOSYL(1-6) O BETA DEXTRO GALACTOPYRANOSYL(1-6) O BETA DEXTRO GALACTOPYRANOSYL(1-6) O BETA DEXTRO GALACTOPYRANOSYL]GLYCEROL; ANTIDIABETIC AGENT; GLUCOSE; GLYCEROL DERIVATIVE; GLYCOLIPID; METFORMIN; STREPTOZOCIN; TETRASACCHARIDE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CONTROLLED STUDY; CUCURBITA MOSCHATA; FRUIT; HETERONUCLEAR MULTIPLE BOND CORRELATION; HETERONUCLEAR SINGLE QUANTUM COHERENCE; INFRARED SPECTROSCOPY; MASS SPECTROMETRY; MEDICINAL PLANT; MOUSE; NONHUMAN; PROTON NUCLEAR MAGNETIC RESONANCE; STREPTOZOCIN DIABETES; STRUCTURE ACTIVITY RELATION;

ANIMALS; BLOOD GLUCOSE; CUCURBITA; DIABETES MELLITUS, EXPERIMENTAL; FRUIT; GLYCOLIPIDS; HYPOGLYCEMIC AGENTS; MAGNETIC RESONANCE SPECTROSCOPY; MICE; MOLECULAR CONFORMATION;

CUCURBITA MOSCHATA; MUS;

EID: 78751642950     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2010.12.030     Document Type: Article

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15. 16 In brief, the animals were fed high-fat diets for 4 weeks before ip injection of 120 mg/kg streptozotocin (STZ, Sigma, USA). An equal volume of vehicle was injected into control mice (n = 10). Serum glucose levels were determined using a glucose measuring kit (Biosino Bio-technology and Science, China), using the glucose oxidase method. Mice with fasting glucose levels >11 mmol/l were considered to be diabetic. Diabetic mice with consecutive 10-day hyperglycemia (≥11 mmol/l) were used for the experiment. The diabetic mice were randomly divided into four groups: QGMG-3-treated, QGMG-2-treated, and metformin-treated groups (n = 10 each), and diabetic group (n = 10). The mice in the QGMG-3- and QGMG-2-treated groups were injected ip with QGMG-3 and QGMG-2, respectively, at doses of 50 mg/kg body weight daily for 4 weeks. The metformin-treated group was injected i.p. with metformin at a dose of 250 mg/kg body weight daily for 4 weeks. The diabetic group was injected ip with vehicle solvent (sterilized 0.9% NaCl). An age- and weight-matched normal group (n = 10) was used as the control group. Body weight and food consumption in each group were monitored daily. Blood glucose levels were checked every 7 days. Blood samples were obtained from the tail vein, and serum glucose levels were determined, as described above. The results are presented as means ± SEM. The statistical methods used to analyze the data in this study were unpaired Student's t-test (two-tailed) using MS-Excel software program. Comparisons with P values <0.05 were considered to be statistically significant.
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